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Article: Survivin depletion inhibits tumor growth and enhances chemosensitivity in hepatocellular carcinoma

TitleSurvivin depletion inhibits tumor growth and enhances chemosensitivity in hepatocellular carcinoma
Authors
KeywordsCisplatin
Tumorigenesis
Survivin
Hepatocellular carcinoma
Chemosensitivity
Issue Date2014
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
Citation
Molecular Medicine Reports, 2014, v. 10 n. 4, p. 2025-2030 How to Cite?
AbstractSurvivin is a member of the inhibitor of apoptosis family, which has been suggested to be crucial in the control of cell division and inhibition of apoptosis. Expression of this protein has been observed in transformed cell lines and human tumor tissues, including those from colorectal cancer, but not in terminally differentiated adult tissues. Survivin mRNA expression has frequently been detected in hepatocellular carcinoma (HCC) and its protein expression has been demonstrated to be highly correlated with proliferation index rather than apoptotic index. The present study aimed to analyze the effect of survivin on the tumorigenicity and chemosensitivity of HCC via the establishment of an HCC cell line (PLC/PRF/5) with the stable knockdown of the survivin gene (PLC-k3). This cell line displayed significantly lower rates of survival and proliferation in assays of cell viability and proliferation, respectively, compared with those of the control cell line (PLC-v). In addition, PLC-k3 cells were more sensitive to cisplatin treatment, resulting in S phase arrest. These findings were further confirmed by an in vivo experiment. The data of the present study suggest that survivin is critical in promoting cell proliferation but not in inhibition of apoptosis, and enhances the chemosensitivity of HCC. Thus, the suppression of survivin expression in combination with cisplatin may contribute to the development of more effective treatments for HCC.
Persistent Identifierhttp://hdl.handle.net/10722/200963
ISSN
2015 Impact Factor: 1.559
2015 SCImago Journal Rankings: 0.637

 

DC FieldValueLanguage
dc.contributor.authorOr, YYY-
dc.contributor.authorChow, AKM-
dc.contributor.authorNg, L-
dc.contributor.authorFan, ST-
dc.contributor.authorYau, TCC-
dc.contributor.authorPoon, RTP-
dc.contributor.authorPang, RWC-
dc.date.accessioned2014-08-21T07:07:45Z-
dc.date.available2014-08-21T07:07:45Z-
dc.date.issued2014-
dc.identifier.citationMolecular Medicine Reports, 2014, v. 10 n. 4, p. 2025-2030-
dc.identifier.issn1791-2997-
dc.identifier.urihttp://hdl.handle.net/10722/200963-
dc.description.abstractSurvivin is a member of the inhibitor of apoptosis family, which has been suggested to be crucial in the control of cell division and inhibition of apoptosis. Expression of this protein has been observed in transformed cell lines and human tumor tissues, including those from colorectal cancer, but not in terminally differentiated adult tissues. Survivin mRNA expression has frequently been detected in hepatocellular carcinoma (HCC) and its protein expression has been demonstrated to be highly correlated with proliferation index rather than apoptotic index. The present study aimed to analyze the effect of survivin on the tumorigenicity and chemosensitivity of HCC via the establishment of an HCC cell line (PLC/PRF/5) with the stable knockdown of the survivin gene (PLC-k3). This cell line displayed significantly lower rates of survival and proliferation in assays of cell viability and proliferation, respectively, compared with those of the control cell line (PLC-v). In addition, PLC-k3 cells were more sensitive to cisplatin treatment, resulting in S phase arrest. These findings were further confirmed by an in vivo experiment. The data of the present study suggest that survivin is critical in promoting cell proliferation but not in inhibition of apoptosis, and enhances the chemosensitivity of HCC. Thus, the suppression of survivin expression in combination with cisplatin may contribute to the development of more effective treatments for HCC.-
dc.languageeng-
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/-
dc.relation.ispartofMolecular Medicine Reports-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCisplatin-
dc.subjectTumorigenesis-
dc.subjectSurvivin-
dc.subjectHepatocellular carcinoma-
dc.subjectChemosensitivity-
dc.titleSurvivin depletion inhibits tumor growth and enhances chemosensitivity in hepatocellular carcinoma-
dc.typeArticle-
dc.identifier.emailOr, YYY: yvonnekin@gmail.com-
dc.identifier.emailChow, AKM: chowakm@hku.hk-
dc.identifier.emailNg, L: luing@hku.hk-
dc.identifier.emailFan, ST: stfan@hku.hk-
dc.identifier.emailYau, TCC: tyaucc@hku.hk-
dc.identifier.emailPoon, RTP: poontp@hku.hk-
dc.identifier.emailPang, RWC: robertap@hku.hk-
dc.identifier.authorityFan, ST=rp00355-
dc.identifier.authorityYau, TCC=rp01466-
dc.identifier.authorityPoon, RTP=rp00446-
dc.identifier.authorityPang, RWC=rp00274-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3892/mmr.2014.2413-
dc.identifier.pmid25070628-
dc.identifier.scopuseid_2-s2.0-84924220267-
dc.identifier.hkuros233820-
dc.identifier.hkuros236955-
dc.identifier.volume10-
dc.identifier.issue4-
dc.identifier.spage2025-
dc.identifier.epage2030-
dc.publisher.placeGreece-

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