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Article: Genome-Wide RNAi Screening Identifies Genes Inhibiting the Migration of Glioblastoma Cells

TitleGenome-Wide RNAi Screening Identifies Genes Inhibiting the Migration of Glioblastoma Cells
Authors
Issue Date2013
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS ONE, 2013, v. 8 n. 4, article no. e61915 How to Cite?
AbstractGlioblastoma Multiforme (GBM) cells are highly invasive, infiltrating into the surrounding normal brain tissue, making it impossible to completely eradicate GBM tumors by surgery or radiation. Increasing evidence also shows that these migratory cells are highly resistant to cytotoxic reagents, but decreasing their migratory capability can re-sensitize them to chemotherapy. These evidences suggest that the migratory cell population may serve as a better therapeutic target for more effective treatment of GBM. In order to understand the regulatory mechanism underlying the motile phenotype, we carried out a genome-wide RNAi screen for genes inhibiting the migration of GBM cells. The screening identified a total of twenty-five primary hits; seven of them were confirmed by secondary screening. Further study showed that three of the genes, FLNA, KHSRP and HCFC1, also functioned in vivo, and knocking them down caused multifocal tumor in a mouse model. Interestingly, two genes, KHSRP and HCFC1, were also found to be correlated with the clinical outcome of GBM patients. These two genes have not been previously associated with cell migration.
Persistent Identifierhttp://hdl.handle.net/10722/200601
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYang, Jen_US
dc.contributor.authorFan, Jen_US
dc.contributor.authorLi, Yen_US
dc.contributor.authorLi, Fen_US
dc.contributor.authorChen, Pen_US
dc.contributor.authorFan, YBen_US
dc.contributor.authorXia, XFen_US
dc.contributor.authorWong, STCen_US
dc.contributor.authorDebinski, Wen_US
dc.date.accessioned2014-08-21T06:52:38Z-
dc.date.available2014-08-21T06:52:38Z-
dc.date.issued2013en_US
dc.identifier.citationPLoS ONE, 2013, v. 8 n. 4, article no. e61915en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/200601-
dc.description.abstractGlioblastoma Multiforme (GBM) cells are highly invasive, infiltrating into the surrounding normal brain tissue, making it impossible to completely eradicate GBM tumors by surgery or radiation. Increasing evidence also shows that these migratory cells are highly resistant to cytotoxic reagents, but decreasing their migratory capability can re-sensitize them to chemotherapy. These evidences suggest that the migratory cell population may serve as a better therapeutic target for more effective treatment of GBM. In order to understand the regulatory mechanism underlying the motile phenotype, we carried out a genome-wide RNAi screen for genes inhibiting the migration of GBM cells. The screening identified a total of twenty-five primary hits; seven of them were confirmed by secondary screening. Further study showed that three of the genes, FLNA, KHSRP and HCFC1, also functioned in vivo, and knocking them down caused multifocal tumor in a mouse model. Interestingly, two genes, KHSRP and HCFC1, were also found to be correlated with the clinical outcome of GBM patients. These two genes have not been previously associated with cell migration.en_US
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_US
dc.relation.ispartofPLoS ONEen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleGenome-Wide RNAi Screening Identifies Genes Inhibiting the Migration of Glioblastoma Cellsen_US
dc.typeArticleen_US
dc.identifier.emailChen, P: pkchen@hku.hken_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0061915en_US
dc.identifier.pmid23593504-
dc.identifier.scopuseid_2-s2.0-84876079529-
dc.identifier.hkuros232133en_US
dc.identifier.volume8en_US
dc.identifier.issue4, article no. e61915-
dc.identifier.isiWOS:000317385300090-
dc.publisher.placeUnited Statesen_US

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