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Article: Effect of different rhBMP-2 and TG-VEGF ratios on the formation of heterotopic bone and neovessels

TitleEffect of different rhBMP-2 and TG-VEGF ratios on the formation of heterotopic bone and neovessels
Authors
Issue Date2014
PublisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.html
Citation
BioMed Research International, 2014, v. 2014, article no. 571510 How to Cite?
AbstractBioengineered bone substitutes might represent alternatives to autologous bone grafts in medically compromised patients due to reduced operation time and comorbidity. Due to the lack of an inherent vascular system their dimension is limited to the size of critical bone size defect. To overcome this shortcoming, the experiment tried to create heterotopic bone around vessels. In vivo, a two-component fibrin and thrombin gel containing recombinant bone morphogenic protein (rhBMP-2) and transglutamate vascular endothelial growth factor (TG-VEGF) in different ratios, respectively, was injected into a dimensionally stable membrane tube, wrapped around the femoral vessel bundle in twelve New Zealand white rabbits. Sacrifice occurred eight weeks postoperatively. Microcomputed tomography of the specimens showed significantly increased bone volume in the rhBMP-2 to TG-VEGF ratio of 10 to 1 group. Histology showed new bone formation in close proximity to the vessel bundle. Immunohistochemistry detected increased angiogenesis within the newly formed bone in the rhBMP-2 to TG-VEGF ratios of 3 to 1 and 5 to 1. Heterotopic bone was engineered in vivo around vessels using different rhBMP-2 and TG-VEGF ratios in a fibrin matrix injected into a dimensionally stable membrane tube which prevented direct contact with skeletal muscles.
Persistent Identifierhttp://hdl.handle.net/10722/200406
ISSN
2015 Impact Factor: 2.134
2015 SCImago Journal Rankings: 0.725
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCai, WXen_US
dc.contributor.authorZheng, LWen_US
dc.contributor.authorLi, CLen_US
dc.contributor.authorMa, Len_US
dc.contributor.authorEhrbar, Men_US
dc.contributor.authorWeber, FEen_US
dc.contributor.authorZwahlen, RAen_US
dc.date.accessioned2014-08-21T06:45:15Z-
dc.date.available2014-08-21T06:45:15Z-
dc.date.issued2014en_US
dc.identifier.citationBioMed Research International, 2014, v. 2014, article no. 571510en_US
dc.identifier.issn2314-6133-
dc.identifier.urihttp://hdl.handle.net/10722/200406-
dc.description.abstractBioengineered bone substitutes might represent alternatives to autologous bone grafts in medically compromised patients due to reduced operation time and comorbidity. Due to the lack of an inherent vascular system their dimension is limited to the size of critical bone size defect. To overcome this shortcoming, the experiment tried to create heterotopic bone around vessels. In vivo, a two-component fibrin and thrombin gel containing recombinant bone morphogenic protein (rhBMP-2) and transglutamate vascular endothelial growth factor (TG-VEGF) in different ratios, respectively, was injected into a dimensionally stable membrane tube, wrapped around the femoral vessel bundle in twelve New Zealand white rabbits. Sacrifice occurred eight weeks postoperatively. Microcomputed tomography of the specimens showed significantly increased bone volume in the rhBMP-2 to TG-VEGF ratio of 10 to 1 group. Histology showed new bone formation in close proximity to the vessel bundle. Immunohistochemistry detected increased angiogenesis within the newly formed bone in the rhBMP-2 to TG-VEGF ratios of 3 to 1 and 5 to 1. Heterotopic bone was engineered in vivo around vessels using different rhBMP-2 and TG-VEGF ratios in a fibrin matrix injected into a dimensionally stable membrane tube which prevented direct contact with skeletal muscles.-
dc.languageengen_US
dc.publisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/jbb/index.html-
dc.relation.ispartofBioMed Research Internationalen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleEffect of different rhBMP-2 and TG-VEGF ratios on the formation of heterotopic bone and neovesselsen_US
dc.typeArticleen_US
dc.identifier.emailZheng, LW: lwzheng@hku.hken_US
dc.identifier.emailMa, L: lima@hku.hken_US
dc.identifier.emailZwahlen, RA: zwahlen@hku.hken_US
dc.identifier.authorityZheng, LW=rp01411en_US
dc.identifier.authorityZwahlen, RA=rp00055en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1155/2014/571510en_US
dc.identifier.pmid24783213-
dc.identifier.pmcidPMC3982283-
dc.identifier.hkuros232383en_US
dc.identifier.volume2014en_US
dc.identifier.isiWOS:000333909500001-
dc.publisher.placeUnited States-

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