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Article: Inhibition of autophagy enhances cisplatin cytotoxicity in human adenoid cystic carcinoma cells of salivary glands

TitleInhibition of autophagy enhances cisplatin cytotoxicity in human adenoid cystic carcinoma cells of salivary glands
Authors
KeywordsAutophagy
Salivary gland
Head and neck cancer
Cisplatin
Chemotherapy
Adenoid cystic carcinoma
Issue Date2013
Citation
Journal of Oral Pathology and Medicine, 2013, v. 42, n. 10, p. 774-780 How to Cite?
AbstractBackground: The relationship between autophagy and chemotherapy in cancer has been studied a lot recent years. However, there is currently no study on the role of autophagy in chemotherapy of adenoid cystic carcinoma (ACC) of human salivary glands. We hypothesized that autophagy plays a protective role for human salivary gland ACC cells during chemotherapy, diminishes the effect of treatment, and ultimately results in poor sensitivity to chemotherapy. Materials and Methods: After inhibition of autophagy by 5 mM 3-methyladenine (3MA), 20 μM Chloroquine (CQ), or Beclin-1 shRNA, we examined the sensitivity of human salivary gland ACC cells to different concentrations of cis-diamminedichloroplatinum (CDDP) using MTT assay. Also, levels of autophagy in ACC cells treated by CDDP were assessed by western blot, GFP-LC3 fluorescence and transmission electron microscopy (TEM). Results: Inhibition of autophagy induced by 3MA, CQ, or Beclin-1 shRNA could all enhance human salivary gland ACC cell death treated by CDDP. And, levels of autophagy in these cells showed a significant increase after treated by CDDP. Conclusion: Autophagy played a protective role for human salivary gland ACC cells during CDDP chemotherapy. Inhibition of autophagy in these cells could enhance cisplatin cytotoxicity-effects. These findings indicate a novel and promising way to reduce chemotherapy resistance and improve treatment outcome in human salivary gland ACC. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/200346
ISSN
2015 Impact Factor: 1.859
2015 SCImago Journal Rankings: 0.731
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMa, Ben-
dc.contributor.authorLiang, Lizhong-
dc.contributor.authorLiao, Guiqing-
dc.contributor.authorLiang, Yujie-
dc.contributor.authorLiu, Haichao-
dc.contributor.authorZheng, Guangsen-
dc.contributor.authorSu, Yuxiong-
dc.date.accessioned2014-08-11T02:42:43Z-
dc.date.available2014-08-11T02:42:43Z-
dc.date.issued2013-
dc.identifier.citationJournal of Oral Pathology and Medicine, 2013, v. 42, n. 10, p. 774-780-
dc.identifier.issn0904-2512-
dc.identifier.urihttp://hdl.handle.net/10722/200346-
dc.description.abstractBackground: The relationship between autophagy and chemotherapy in cancer has been studied a lot recent years. However, there is currently no study on the role of autophagy in chemotherapy of adenoid cystic carcinoma (ACC) of human salivary glands. We hypothesized that autophagy plays a protective role for human salivary gland ACC cells during chemotherapy, diminishes the effect of treatment, and ultimately results in poor sensitivity to chemotherapy. Materials and Methods: After inhibition of autophagy by 5 mM 3-methyladenine (3MA), 20 μM Chloroquine (CQ), or Beclin-1 shRNA, we examined the sensitivity of human salivary gland ACC cells to different concentrations of cis-diamminedichloroplatinum (CDDP) using MTT assay. Also, levels of autophagy in ACC cells treated by CDDP were assessed by western blot, GFP-LC3 fluorescence and transmission electron microscopy (TEM). Results: Inhibition of autophagy induced by 3MA, CQ, or Beclin-1 shRNA could all enhance human salivary gland ACC cell death treated by CDDP. And, levels of autophagy in these cells showed a significant increase after treated by CDDP. Conclusion: Autophagy played a protective role for human salivary gland ACC cells during CDDP chemotherapy. Inhibition of autophagy in these cells could enhance cisplatin cytotoxicity-effects. These findings indicate a novel and promising way to reduce chemotherapy resistance and improve treatment outcome in human salivary gland ACC. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.-
dc.languageeng-
dc.relation.ispartofJournal of Oral Pathology and Medicine-
dc.subjectAutophagy-
dc.subjectSalivary gland-
dc.subjectHead and neck cancer-
dc.subjectCisplatin-
dc.subjectChemotherapy-
dc.subjectAdenoid cystic carcinoma-
dc.titleInhibition of autophagy enhances cisplatin cytotoxicity in human adenoid cystic carcinoma cells of salivary glands-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/jop.12066-
dc.identifier.pmid23590333-
dc.identifier.scopuseid_2-s2.0-84887246254-
dc.identifier.volume42-
dc.identifier.issue10-
dc.identifier.spage774-
dc.identifier.epage780-
dc.identifier.eissn1600-0714-
dc.identifier.isiWOS:000326815200009-

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