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Article: Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR

TitleSimultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR
Authors
Issue Date1999
Citation
Journal of Clinical Microbiology, 1999, v. 37, n. 5, p. 1621-1624 How to Cite?
AbstractIn a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation.
Persistent Identifierhttp://hdl.handle.net/10722/200070
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorConrads, Georg-
dc.contributor.authorFlemmig, Thomas Frank-
dc.contributor.authorSeyfarth, Ilse-
dc.contributor.authorLampert, Fritz H.-
dc.contributor.authorLütticken, Rudolf-
dc.date.accessioned2014-07-26T23:11:06Z-
dc.date.available2014-07-26T23:11:06Z-
dc.date.issued1999-
dc.identifier.citationJournal of Clinical Microbiology, 1999, v. 37, n. 5, p. 1621-1624-
dc.identifier.issn0095-1137-
dc.identifier.urihttp://hdl.handle.net/10722/200070-
dc.description.abstractIn a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation.-
dc.languageeng-
dc.relation.ispartofJournal of Clinical Microbiology-
dc.titleSimultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10203541-
dc.identifier.scopuseid_2-s2.0-0032895585-
dc.identifier.volume37-
dc.identifier.issue5-
dc.identifier.spage1621-
dc.identifier.epage1624-
dc.identifier.isiWOS:000079792400079-

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