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Article: Dual inhibitory and stimulatory activities in serum from SLE patients and lupus mice that regulate the proliferation of an IL-2-dependent T cell line

TitleDual inhibitory and stimulatory activities in serum from SLE patients and lupus mice that regulate the proliferation of an IL-2-dependent T cell line
Authors
KeywordsInhibitory factor
IL-2
CTLL
Stimulatory factor
Issue Date1995
Citation
Lupus, 1995, v. 4, n. 4, p. 297-303 How to Cite?
AbstractIn serum and plasma from SLE patients, we have detected elevated levels of factors which regulate proliferative responses of CTLL cells to IL-2. Serum samples containing these factors have dose-dependent dual inhibitory and stimulatory activities on the proliferation of this IL-2-dependent T lymphocyte cell line. At high concentrations, the serum factors inhibit the proliferative responses of CTLL cells to IL-2. At low concentrations, they synergise with IL-2 stimulating the growth of cells. Similar inhibitory activity, but with lower titre, was also found to be elevated in sera of some MRL/lpr mice, an animal model of SLE. Functional characterisation of the serum factors shows that: (1) the inhibitory activity cannot be neutralised by exogenous IL-2; (2) the stimulatory activity is not due to the presence of serum IL-2 but synergy of the factor with IL-2; (3) the factors bind directly to CTLL cells but they do not bind to protein A; and (4) the serum factors are not dialysable but heat labile. The possible pathological implications of the serum factors, particularly for the defective T cell functions in lupus disease, are discussed.
Persistent Identifierhttp://hdl.handle.net/10722/200049
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHuang, Fangping-
dc.contributor.authorStott, David Ian-
dc.date.accessioned2014-07-26T23:11:04Z-
dc.date.available2014-07-26T23:11:04Z-
dc.date.issued1995-
dc.identifier.citationLupus, 1995, v. 4, n. 4, p. 297-303-
dc.identifier.urihttp://hdl.handle.net/10722/200049-
dc.description.abstractIn serum and plasma from SLE patients, we have detected elevated levels of factors which regulate proliferative responses of CTLL cells to IL-2. Serum samples containing these factors have dose-dependent dual inhibitory and stimulatory activities on the proliferation of this IL-2-dependent T lymphocyte cell line. At high concentrations, the serum factors inhibit the proliferative responses of CTLL cells to IL-2. At low concentrations, they synergise with IL-2 stimulating the growth of cells. Similar inhibitory activity, but with lower titre, was also found to be elevated in sera of some MRL/lpr mice, an animal model of SLE. Functional characterisation of the serum factors shows that: (1) the inhibitory activity cannot be neutralised by exogenous IL-2; (2) the stimulatory activity is not due to the presence of serum IL-2 but synergy of the factor with IL-2; (3) the factors bind directly to CTLL cells but they do not bind to protein A; and (4) the serum factors are not dialysable but heat labile. The possible pathological implications of the serum factors, particularly for the defective T cell functions in lupus disease, are discussed.-
dc.languageeng-
dc.relation.ispartofLupus-
dc.subjectInhibitory factor-
dc.subjectIL-2-
dc.subjectCTLL-
dc.subjectStimulatory factor-
dc.titleDual inhibitory and stimulatory activities in serum from SLE patients and lupus mice that regulate the proliferation of an IL-2-dependent T cell line-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1177/096120339500400411-
dc.identifier.pmid8528227-
dc.identifier.scopuseid_2-s2.0-0029075947-
dc.identifier.volume4-
dc.identifier.issue4-
dc.identifier.spage297-
dc.identifier.epage303-
dc.identifier.isiWOS:A1995RR88700011-

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