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Conference Paper: Consecutive Amphotericin B Treatments Fail to Eradicate Candida Biofilm Persisters

TitleConsecutive Amphotericin B Treatments Fail to Eradicate Candida Biofilm Persisters
Authors
KeywordsBiofilm
Fungi
Infection
Pathogenicity and Therapeutics
Issue Date2014
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1270 How to Cite?
AbstractObjective: Candida infection is a common and critical problem with high morbidity and mortality worldwide. This study attempted to identify the profile of Candida biofilm persisters and establish a reliable in vitro model for their molecular analysis. Method: A total of four well-defined strains from three representative Candida species were employed, including C. albicans SC5314, C. albicans BF-1, C. glabrata ATCC 90030 and C. tropicalis ATCC 13803. These Candida biofilms were established on 24-well polystyrene plates respectively. Their antifungal susceptibility and MICs were determined by XTT reduction assay after treatments with different concentrations of Amphotericin B (1-256 μg/ml) for 24 h. Following 24 h treatment with 256 μg/ml of Amphotericin B, the Candida biofilms were challenged consecutively for another 72 h. The level of persisters was quantified by spiral plating for CFU counting. The cellular viability of Candida biofilms was confirmed by Live/Dead staining and confocal laser scanning microscopy. Result: Overall, Amphotericin B displayed potent antifungal effects on the four Candida biofilms tested (MICs: 1 to 32 μg/ml). The 24 h-treatment with Amphotericin B at 256 μg/ml effectively killed majority of these Candida biofilms, and a small fraction of persister cells (0.01% to 0.62%) survived. Interestingly, consecutive treatments further with 256 μg/ml of Amphotericin B at 48 h, 72 h and 96 h failed to control these biofilms. The viable persister cells were confirmed by confocal imaging. Conclusion: The pioneering study shows that consecutive Amphotericin B treatments were unable to eradicate the biofilm persisters of C. albicans SC5314, C. albicans BF-1, C. glabrata ATCC 90030 and C. tropicalis ATCC 13803. These in vitro models of persisters may facilitate further investigation on the pathogenesis of Candida infection and antifungal strategies. This abstract is based on research that was funded entirely or partially by an outside source: Supported by Hong Kong HMRF Grant, No.12110752, and the Modern Dental Laboratory/HKU Endowment Fund to LJJ
DescriptionPoster Presentation
Session 169: Candida
Persistent Identifierhttp://hdl.handle.net/10722/199336
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorLi, Pen_US
dc.contributor.authorSeneviratne, CJen_US
dc.contributor.authorJin, Len_US
dc.date.accessioned2014-07-22T01:13:40Z-
dc.date.available2014-07-22T01:13:40Z-
dc.date.issued2014en_US
dc.identifier.citationThe 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1270en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/199336-
dc.descriptionPoster Presentation-
dc.descriptionSession 169: Candida-
dc.description.abstractObjective: Candida infection is a common and critical problem with high morbidity and mortality worldwide. This study attempted to identify the profile of Candida biofilm persisters and establish a reliable in vitro model for their molecular analysis. Method: A total of four well-defined strains from three representative Candida species were employed, including C. albicans SC5314, C. albicans BF-1, C. glabrata ATCC 90030 and C. tropicalis ATCC 13803. These Candida biofilms were established on 24-well polystyrene plates respectively. Their antifungal susceptibility and MICs were determined by XTT reduction assay after treatments with different concentrations of Amphotericin B (1-256 μg/ml) for 24 h. Following 24 h treatment with 256 μg/ml of Amphotericin B, the Candida biofilms were challenged consecutively for another 72 h. The level of persisters was quantified by spiral plating for CFU counting. The cellular viability of Candida biofilms was confirmed by Live/Dead staining and confocal laser scanning microscopy. Result: Overall, Amphotericin B displayed potent antifungal effects on the four Candida biofilms tested (MICs: 1 to 32 μg/ml). The 24 h-treatment with Amphotericin B at 256 μg/ml effectively killed majority of these Candida biofilms, and a small fraction of persister cells (0.01% to 0.62%) survived. Interestingly, consecutive treatments further with 256 μg/ml of Amphotericin B at 48 h, 72 h and 96 h failed to control these biofilms. The viable persister cells were confirmed by confocal imaging. Conclusion: The pioneering study shows that consecutive Amphotericin B treatments were unable to eradicate the biofilm persisters of C. albicans SC5314, C. albicans BF-1, C. glabrata ATCC 90030 and C. tropicalis ATCC 13803. These in vitro models of persisters may facilitate further investigation on the pathogenesis of Candida infection and antifungal strategies. This abstract is based on research that was funded entirely or partially by an outside source: Supported by Hong Kong HMRF Grant, No.12110752, and the Modern Dental Laboratory/HKU Endowment Fund to LJJ-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectBiofilm-
dc.subjectFungi-
dc.subjectInfection-
dc.subjectPathogenicity and Therapeutics-
dc.titleConsecutive Amphotericin B Treatments Fail to Eradicate Candida Biofilm Persistersen_US
dc.typeConference_Paperen_US
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_US
dc.identifier.authorityJin, L=rp00028en_US
dc.identifier.hkuros231075en_US
dc.identifier.volume93en_US
dc.identifier.issueSpecial issue B: abstract no. 1270en_US
dc.publisher.placeUnited States-

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