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Conference Paper: Development of Novel 3D Stem Cell Sheet for Periodontal Regeneration

TitleDevelopment of Novel 3D Stem Cell Sheet for Periodontal Regeneration
Authors
KeywordsCell biology
Periodontal disease
Regeneration
Stem cells and Tissue engineering
Issue Date2014
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1067 How to Cite?
AbstractObjective: To assess the regenerative capacity of 3D periodontal ligament stem cell (PDLSC) sheet in vivo. Method: Single culture and co-culture cell sheets were prepared by using PDLSCs and Human umbilical vein endothelial cells (HUVECs) in thermo-responsive culture dishes. Scanning electron microscopy (SEM) was used to observe the ultra-structure of single and co-culture groups of cell sheets. Cells were labeled with cell-tracker dyes (PDLSCs-Green, HUVECs-Red) and seeded in different ratios to assess the time dependent modulation of vascular network formation on Matrigel (4 and 8 hours). Tooth root fragments were coated with PDLSC sheets and implanted into the subcutaneous space of SCID mice. After 2 weeks, implants were retrieved and processed for histological evaluation. Result: SEM results showed the ultra-structural arrangement and preserved extracellular matrix (ECM) of layered cell sheet constructs. Furthermore, increased ECM deposition was observed in co-culture groups. PDLSCs and HUVECs seeded on Matrigel showed initiation of vessel like structure formation after 4 hours. Extensive lattice of vessel like structures was formed in 1:2 and 1:5 groups compared to other groups. However, after 8 hours all the groups showed formation of island like cell aggregations. In vivo, H&E sections exemplified the successful integration of implanted PDL cell sheet coated roots with host tissues after 2 weeks. Periodontal ligament-like arrangement was observed around root surface in cell sheet groups compared to control groups. Conclusions: 3D cell sheet model demonstrated a promising regenerative potential in vivo and this approach would be useful in future periodontal bioengineering strategies. This abstract is based on research that was funded entirely or partially by an outside source: Seed Funding for Basic Research 201111159191 The University of Hong Kong
DescriptionPoster Presentation
Session 151: Periodontal Tissue Regeneration
Persistent Identifierhttp://hdl.handle.net/10722/199331
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorPanduwawala, CPen_US
dc.contributor.authorSamaranayake, LPen_US
dc.contributor.authorJin, Len_US
dc.contributor.authorZhang, Cen_US
dc.date.accessioned2014-07-22T01:13:40Z-
dc.date.available2014-07-22T01:13:40Z-
dc.date.issued2014en_US
dc.identifier.citationThe 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1067en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/199331-
dc.descriptionPoster Presentation-
dc.descriptionSession 151: Periodontal Tissue Regeneration-
dc.description.abstractObjective: To assess the regenerative capacity of 3D periodontal ligament stem cell (PDLSC) sheet in vivo. Method: Single culture and co-culture cell sheets were prepared by using PDLSCs and Human umbilical vein endothelial cells (HUVECs) in thermo-responsive culture dishes. Scanning electron microscopy (SEM) was used to observe the ultra-structure of single and co-culture groups of cell sheets. Cells were labeled with cell-tracker dyes (PDLSCs-Green, HUVECs-Red) and seeded in different ratios to assess the time dependent modulation of vascular network formation on Matrigel (4 and 8 hours). Tooth root fragments were coated with PDLSC sheets and implanted into the subcutaneous space of SCID mice. After 2 weeks, implants were retrieved and processed for histological evaluation. Result: SEM results showed the ultra-structural arrangement and preserved extracellular matrix (ECM) of layered cell sheet constructs. Furthermore, increased ECM deposition was observed in co-culture groups. PDLSCs and HUVECs seeded on Matrigel showed initiation of vessel like structure formation after 4 hours. Extensive lattice of vessel like structures was formed in 1:2 and 1:5 groups compared to other groups. However, after 8 hours all the groups showed formation of island like cell aggregations. In vivo, H&E sections exemplified the successful integration of implanted PDL cell sheet coated roots with host tissues after 2 weeks. Periodontal ligament-like arrangement was observed around root surface in cell sheet groups compared to control groups. Conclusions: 3D cell sheet model demonstrated a promising regenerative potential in vivo and this approach would be useful in future periodontal bioengineering strategies. This abstract is based on research that was funded entirely or partially by an outside source: Seed Funding for Basic Research 201111159191 The University of Hong Kong-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectCell biology-
dc.subjectPeriodontal disease-
dc.subjectRegeneration-
dc.subjectStem cells and Tissue engineering-
dc.titleDevelopment of Novel 3D Stem Cell Sheet for Periodontal Regenerationen_US
dc.typeConference_Paperen_US
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_US
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_US
dc.identifier.emailZhang, C: zhangcf@hku.hken_US
dc.identifier.authoritySamaranayake, LP=rp00023en_US
dc.identifier.authorityJin, L=rp00028en_US
dc.identifier.authorityZhang, C=rp01408en_US
dc.identifier.hkuros231069en_US
dc.identifier.volume93en_US
dc.identifier.issueSpecial issue B: abstract no. 1067en_US
dc.publisher.placeUnited States-

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