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Article: Adrenomedullin increases the short-circuit current in the rat prostate: Receptors, chloride channels, the effects of cAMP and calcium ions and implications on fluid secretion

TitleAdrenomedullin increases the short-circuit current in the rat prostate: Receptors, chloride channels, the effects of cAMP and calcium ions and implications on fluid secretion
Authors
Issue Date2014
Citation
Andrology, 2014, v. 2, p. 474-480 How to Cite?
Abstractn this study, we have investigated the effects of adrenomedullin on chloride and fluid secretion in the rat prostate. The presence of adrenomedullin (ADM) in rat prostate was confirmed using immunostaining, and the molecular species was determined using gel filtration chromatography coupled with an enzyme-linked assay for ADM. The effects of ADM on fluid secretion were studied by short-circuit current technique in a whole mount preparation of the prostate in an Ussing chamber. The results indicated that the ADM level was higher in the ventral than the dorso-lateral prostate and the major molecular species was the active peptide. ADM increased the short-circuit current through both the cAMP- and calcium-activated chloride channels in the ventral lobe, but only through the calcium-activated channels in the dorso-lateral lobe. These stimulatory effects were blocked by the calcitonin gene-related peptide (CGRP) receptor antagonist, hCGRP8-37. We conclude that ADM may regulate prostatic fluid secretion through the chloride channels, which may affect the composition of the seminal plasma bathing the spermatozoa and hence fertility.
Persistent Identifierhttp://hdl.handle.net/10722/198980
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiao, Sen_US
dc.contributor.authorCheung, KHen_US
dc.contributor.authorCheung, MPLen_US
dc.contributor.authorWong, PFen_US
dc.contributor.authorO, WSen_US
dc.contributor.authorTang, Fen_US
dc.date.accessioned2014-07-22T00:58:49Z-
dc.date.available2014-07-22T00:58:49Z-
dc.date.issued2014en_US
dc.identifier.citationAndrology, 2014, v. 2, p. 474-480en_US
dc.identifier.urihttp://hdl.handle.net/10722/198980-
dc.description.abstractn this study, we have investigated the effects of adrenomedullin on chloride and fluid secretion in the rat prostate. The presence of adrenomedullin (ADM) in rat prostate was confirmed using immunostaining, and the molecular species was determined using gel filtration chromatography coupled with an enzyme-linked assay for ADM. The effects of ADM on fluid secretion were studied by short-circuit current technique in a whole mount preparation of the prostate in an Ussing chamber. The results indicated that the ADM level was higher in the ventral than the dorso-lateral prostate and the major molecular species was the active peptide. ADM increased the short-circuit current through both the cAMP- and calcium-activated chloride channels in the ventral lobe, but only through the calcium-activated channels in the dorso-lateral lobe. These stimulatory effects were blocked by the calcitonin gene-related peptide (CGRP) receptor antagonist, hCGRP8-37. We conclude that ADM may regulate prostatic fluid secretion through the chloride channels, which may affect the composition of the seminal plasma bathing the spermatozoa and hence fertility.en_US
dc.languageengen_US
dc.relation.ispartofAndrologyen_US
dc.titleAdrenomedullin increases the short-circuit current in the rat prostate: Receptors, chloride channels, the effects of cAMP and calcium ions and implications on fluid secretionen_US
dc.typeArticleen_US
dc.identifier.emailLiao, S: subin@hkucc.hku.hken_US
dc.identifier.emailCheung, KH: ckingho@hku.hken_US
dc.identifier.emailCheung, MPL: mplcheun@hkucc.hku.hken_US
dc.identifier.emailWong, PF: pfwong@hku.hken_US
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_US
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_US
dc.identifier.authorityCheung, KH=rp01463en_US
dc.identifier.authorityO, WS=rp00315en_US
dc.identifier.authorityTang, F=rp00327en_US
dc.identifier.doi10.1111/j.2047-2927.2014.00189.xen_US
dc.identifier.pmid24711244-
dc.identifier.hkuros231038en_US
dc.identifier.volume2en_US
dc.identifier.spage474en_US
dc.identifier.epage480en_US
dc.identifier.isiWOS:000334881300024-

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