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Article: Mesangial cell-binding activity of serum immunoglobulin G in patients with lupus nephritis

TitleMesangial cell-binding activity of serum immunoglobulin G in patients with lupus nephritis
Authors
Issue Date2014
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2014, v. 9 n. 7, article no. e101987 How to Cite?
AbstractIn vitro data showed that immunoglobulin G (IgG) from patients with lupus nephritis (LN) could bind to cultured human mesangial cells (HMC). The clinical relevance of such binding was unknown. Binding of IgG and subclasses was measured in 189 serial serum samples from 23 patients with Class III/IV+/-V LN (48 during renal flares, 141 during low level disease activity (LLDA)). 64 patients with non-lupus glomerular diseases (NLGD) and 23 healthy individuals were used as controls. HMC-binding was measured with cellular ELISA and expressed as OD index. HMC-binding index of total IgG was 0.12+/-0.09, 0.36+/-0.25, 0.59+/-0.37 and 0.74+/-0.42 in healthy subjects, NLGD, LN patients during LLDA, and LN flares respectively (P = 0.046, LN flare vs. LLDA; P<0.001, for healthy controls or NLGD vs. LN during flare or LLDA). Binding of serum IgG1 to HMC was 0.05+/-0.05, 0.15+/-0.11, 0.41+/-0.38 and 0.55+/-0.40 for the corresponding groups respectively (P = 0.007, LN flare vs. remission; P<0.001, for healthy controls or NLGD vs. LN during flare or remission). IgG2, IgG3 and IgG4 from patients and controls did not show significant binding to HMC. Total IgG and IgG1 HMC-binding index correlated with anti-dsDNA level (r = 0.26 and 0.39 respectively, P<0.001 for both), and inversely with C3 (r = -0.17 and -0.45, P<0.05 for both). Sensitivity/specificity of total IgG or IgG1 binding to HMC in predicting renal flares were 81.3%/39.7% (ROC AUC 0.61, P = 0.03) and 83.8%/41.8% (AUC 0.63, P = 0.009) respectively. HMC-binding by IgG1, but not total IgG, correlated with mesangial immune deposition in LN renal biopsies under electron microscopy. Our results showed that binding of serum total IgG and IgG1 in LN patients correlates with disease activity. The correlation between IgG1 HMC-binding and mesangial immune deposition suggests a potential pathogenic significance.
Persistent Identifierhttp://hdl.handle.net/10722/198544
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYap, DYHen_US
dc.contributor.authorYung, SSYen_US
dc.contributor.authorZhang, Qen_US
dc.contributor.authorTang, Cen_US
dc.contributor.authorChan, DTM-
dc.date.accessioned2014-07-07T07:17:10Z-
dc.date.available2014-07-07T07:17:10Z-
dc.date.issued2014-
dc.identifier.citationPLoS One, 2014, v. 9 n. 7, article no. e101987en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/198544-
dc.description.abstractIn vitro data showed that immunoglobulin G (IgG) from patients with lupus nephritis (LN) could bind to cultured human mesangial cells (HMC). The clinical relevance of such binding was unknown. Binding of IgG and subclasses was measured in 189 serial serum samples from 23 patients with Class III/IV+/-V LN (48 during renal flares, 141 during low level disease activity (LLDA)). 64 patients with non-lupus glomerular diseases (NLGD) and 23 healthy individuals were used as controls. HMC-binding was measured with cellular ELISA and expressed as OD index. HMC-binding index of total IgG was 0.12+/-0.09, 0.36+/-0.25, 0.59+/-0.37 and 0.74+/-0.42 in healthy subjects, NLGD, LN patients during LLDA, and LN flares respectively (P = 0.046, LN flare vs. LLDA; P<0.001, for healthy controls or NLGD vs. LN during flare or LLDA). Binding of serum IgG1 to HMC was 0.05+/-0.05, 0.15+/-0.11, 0.41+/-0.38 and 0.55+/-0.40 for the corresponding groups respectively (P = 0.007, LN flare vs. remission; P<0.001, for healthy controls or NLGD vs. LN during flare or remission). IgG2, IgG3 and IgG4 from patients and controls did not show significant binding to HMC. Total IgG and IgG1 HMC-binding index correlated with anti-dsDNA level (r = 0.26 and 0.39 respectively, P<0.001 for both), and inversely with C3 (r = -0.17 and -0.45, P<0.05 for both). Sensitivity/specificity of total IgG or IgG1 binding to HMC in predicting renal flares were 81.3%/39.7% (ROC AUC 0.61, P = 0.03) and 83.8%/41.8% (AUC 0.63, P = 0.009) respectively. HMC-binding by IgG1, but not total IgG, correlated with mesangial immune deposition in LN renal biopsies under electron microscopy. Our results showed that binding of serum total IgG and IgG1 in LN patients correlates with disease activity. The correlation between IgG1 HMC-binding and mesangial immune deposition suggests a potential pathogenic significance.-
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleMesangial cell-binding activity of serum immunoglobulin G in patients with lupus nephritisen_US
dc.typeArticleen_US
dc.identifier.emailYap, DYH: desmondy@hku.hken_US
dc.identifier.emailYung, SSY: ssyyung@hku.hken_US
dc.identifier.emailTang, C: csotang@HKUCC.hku.hken_US
dc.identifier.emailChan, DTM: dtmchan@hku.hk-
dc.identifier.authorityYap, DYH=rp01607en_US
dc.identifier.authorityYung, SSY=rp00455en_US
dc.identifier.authorityChan, DTM=rp00394en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0101987-
dc.identifier.pmid25007094-
dc.identifier.pmcidPMC4090162-
dc.identifier.hkuros239869en_US
dc.identifier.volume9-
dc.identifier.issue7, article no. e101987-
dc.identifier.isiWOS:000339040600086-
dc.publisher.placeUnited States-
dc.customcontrol.immutablecsl 150121-

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