File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: microRNA-146 up-regulation predicts the prognosis of non-small cell lung cancer by miRNA in situ hybridization

TitlemicroRNA-146 up-regulation predicts the prognosis of non-small cell lung cancer by miRNA in situ hybridization
Authors
Issue Date2014
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexmp
Citation
Experimental and Molecular Pathology, 2014, v. 96 n. 2, p. 195-199 How to Cite?
AbstractNon-small cell lung cancer (NSCLC) accounts for approximately 70% of all lung cancer-related deaths worldwide. Prognostic markers are essential for the early detection of lung cancer in patients. In this study, we first identified microRNA146 (miR-146) expression in cancer cell lines using miRNA in situ hybridization (MISH) and confirmed the accuracy of MISH using q-RT-PCR. In addition, two different systems, BCIP/NBT and ELF, were used to detect the signal for a comparative analysis of the specificity of MISH. Compared to the BCIP/NBT system, the ELF detection system was more effective for MISH. Furthermore we detected the expression of miR-146 in NSCLC tissues (43 cases) and normal tissues (32 cases). Based on our results, we can conclude that miR-146 is more highly expressed in cancer tissue than normal tissue (t-test, P<0.05) and that miR-146 can predict the prognosis of NSCLC by MISH. Our findings preliminary demonstrate that MISH can be applied as a molecular diagnostic tool to determine the expression and localization of miRNAs in cancer tissues and that miR-146, determined by MISH, predicts the prognosis of NSCLC patients.
Persistent Identifierhttp://hdl.handle.net/10722/198473
ISSN
2015 Impact Factor: 2.638
2015 SCImago Journal Rankings: 1.130
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, J-
dc.contributor.authorYang, H-
dc.contributor.authorLi, Y-
dc.contributor.authorLiu, Y-
dc.contributor.authorChen, S-
dc.contributor.authorQi, C-
dc.contributor.authorZhang, Q-
dc.contributor.authorLan, T-
dc.contributor.authorHe, X-
dc.contributor.authorGuan, X-
dc.contributor.authorWang, L-
dc.date.accessioned2014-07-07T07:08:24Z-
dc.date.available2014-07-07T07:08:24Z-
dc.date.issued2014-
dc.identifier.citationExperimental and Molecular Pathology, 2014, v. 96 n. 2, p. 195-199-
dc.identifier.issn0014-4800-
dc.identifier.urihttp://hdl.handle.net/10722/198473-
dc.description.abstractNon-small cell lung cancer (NSCLC) accounts for approximately 70% of all lung cancer-related deaths worldwide. Prognostic markers are essential for the early detection of lung cancer in patients. In this study, we first identified microRNA146 (miR-146) expression in cancer cell lines using miRNA in situ hybridization (MISH) and confirmed the accuracy of MISH using q-RT-PCR. In addition, two different systems, BCIP/NBT and ELF, were used to detect the signal for a comparative analysis of the specificity of MISH. Compared to the BCIP/NBT system, the ELF detection system was more effective for MISH. Furthermore we detected the expression of miR-146 in NSCLC tissues (43 cases) and normal tissues (32 cases). Based on our results, we can conclude that miR-146 is more highly expressed in cancer tissue than normal tissue (t-test, P<0.05) and that miR-146 can predict the prognosis of NSCLC by MISH. Our findings preliminary demonstrate that MISH can be applied as a molecular diagnostic tool to determine the expression and localization of miRNAs in cancer tissues and that miR-146, determined by MISH, predicts the prognosis of NSCLC patients.-
dc.languageeng-
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexmp-
dc.relation.ispartofExperimental and Molecular Pathology-
dc.subject.meshCarcinoma, Non-Small-Cell Lung - genetics - pathology-
dc.subject.meshGene Expression Regulation, Neoplastic-
dc.subject.meshMicroRNAs - biosynthesis - genetics-
dc.subject.meshPrognosis-
dc.subject.meshTumor Markers, Biological - genetics-
dc.titlemicroRNA-146 up-regulation predicts the prognosis of non-small cell lung cancer by miRNA in situ hybridization-
dc.typeArticle-
dc.identifier.emailGuan, X: xyguan@hkucc.hku.hk-
dc.identifier.authorityGuan, X=rp00454-
dc.identifier.doi10.1016/j.yexmp.2013.11.004-
dc.identifier.pmid24448024-
dc.identifier.scopuseid_2-s2.0-84894038026-
dc.identifier.hkuros229766-
dc.identifier.volume96-
dc.identifier.issue2-
dc.identifier.spage195-
dc.identifier.epage199-
dc.identifier.isiWOS:000334655100009-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats