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Article: Global transcriptome analysis of AtPAP2--overexpressing Arabidopsis thaliana with elevated ATP

TitleGlobal transcriptome analysis of AtPAP2--overexpressing Arabidopsis thaliana with elevated ATP
Authors
Issue Date2013
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcgenomics/
Citation
BMC Genomics, 2013, v. 14, article no. 752 How to Cite?
AbstractBACKGROUND: AtPAP2 is a purple acid phosphatase that is targeted to both chloroplasts and mitochondria. Over-expression (OE) lines of AtPAP2 grew faster, produced more seeds, and contained higher leaf sucrose and glucose contents. The present study aimed to determine how high energy status affects leaf and root transcriptomes. RESULTS: ATP and ADP levels in the OE lines are 30-50% and 20-50% higher than in the wild-type (WT) plants. Global transcriptome analyses indicated that transcriptional regulation does play a role in sucrose and starch metabolism, nitrogen, potassium and iron uptake, amino acids and secondary metabolites metabolism when there is an ample supply of energy. While the transcript abundance of genes encoding protein components of photosystem I (PS I), photosystem II (PS II) and light harvesting complex I (LHCI) were unaltered, changes in transcript abundance for genes encoding proteins of LHCII are significant. The gene expressions of most enzymes of the Calvin cycle, glycolysis and the tricarboxylic acid (TCA) cycle were unaltered, as these enzymes are known to be regulated by light/redox status or allosteric modulation by the products (e.g. citrate, ATP/ADP ratio), but not at the level of transcription. CONCLUSIONS: AtPAP2 overexpression resulted in a widespread reprogramming of the transcriptome in the transgenic plants, which is characterized by changes in the carbon, nitrogen, potassium, and iron metabolism. The fast-growing AtPAP2 OE lines provide an interesting tool for studying the regulation of energy system in plant.
Persistent Identifierhttp://hdl.handle.net/10722/198227
ISSN
2015 Impact Factor: 3.867
2015 SCImago Journal Rankings: 2.343
ISI Accession Number ID
Grants

 

DC FieldValueLanguage
dc.contributor.authorSun, Fen_US
dc.contributor.authorLiang, Cen_US
dc.contributor.authorWhelan, Jen_US
dc.contributor.authorYang, Jen_US
dc.contributor.authorZhang, Pen_US
dc.contributor.authorLim, BLen_US
dc.date.accessioned2014-06-25T02:56:43Z-
dc.date.available2014-06-25T02:56:43Z-
dc.date.issued2013en_US
dc.identifier.citationBMC Genomics, 2013, v. 14, article no. 752en_US
dc.identifier.issn1471-2164en_US
dc.identifier.urihttp://hdl.handle.net/10722/198227-
dc.description.abstractBACKGROUND: AtPAP2 is a purple acid phosphatase that is targeted to both chloroplasts and mitochondria. Over-expression (OE) lines of AtPAP2 grew faster, produced more seeds, and contained higher leaf sucrose and glucose contents. The present study aimed to determine how high energy status affects leaf and root transcriptomes. RESULTS: ATP and ADP levels in the OE lines are 30-50% and 20-50% higher than in the wild-type (WT) plants. Global transcriptome analyses indicated that transcriptional regulation does play a role in sucrose and starch metabolism, nitrogen, potassium and iron uptake, amino acids and secondary metabolites metabolism when there is an ample supply of energy. While the transcript abundance of genes encoding protein components of photosystem I (PS I), photosystem II (PS II) and light harvesting complex I (LHCI) were unaltered, changes in transcript abundance for genes encoding proteins of LHCII are significant. The gene expressions of most enzymes of the Calvin cycle, glycolysis and the tricarboxylic acid (TCA) cycle were unaltered, as these enzymes are known to be regulated by light/redox status or allosteric modulation by the products (e.g. citrate, ATP/ADP ratio), but not at the level of transcription. CONCLUSIONS: AtPAP2 overexpression resulted in a widespread reprogramming of the transcriptome in the transgenic plants, which is characterized by changes in the carbon, nitrogen, potassium, and iron metabolism. The fast-growing AtPAP2 OE lines provide an interesting tool for studying the regulation of energy system in plant.en_US
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcgenomics/-
dc.relation.ispartofBMC Genomicsen_US
dc.rightsBMC Genomics. Copyright © BioMed Central Ltd.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleGlobal transcriptome analysis of AtPAP2--overexpressing Arabidopsis thaliana with elevated ATPen_US
dc.typeArticleen_US
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_US
dc.identifier.authorityLim, BL=rp00744en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1471-2164-14-752en_US
dc.identifier.pmid24180234-
dc.identifier.hkuros229140en_US
dc.identifier.volume14en_US
dc.identifier.spage752en_US
dc.identifier.epage752en_US
dc.identifier.isiWOS:000328636200008-
dc.relation.projectHow does a purple acid phosphatase promote plant growth?-

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