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Article: Differential expression of Toll-like receptor 4 in healthy and diseased human gingiva

TitleDifferential expression of Toll-like receptor 4 in healthy and diseased human gingiva
Authors
Issue Date2014
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal of Periodontal Research, 2014, v. 49 n. 6, p. 845–854 How to Cite?
AbstractBACKGROUND AND OBJECTIVE: Lipopolysaccharide (LPS)-mediated signaling in host cells involves Toll-like receptor 4 (TLR4) accessory molecules, including LPS-binding protein (LBP), cluster of differentiation 14 (CD14) and lymphocyte antigen 96 (MD-2). However, expression of these innate defense molecules in various compartments of the human periodontium is unclear. The aim of this study was to investigate the expression profile of TLR4 in human gingiva. MATERIAL AND METHODS: Human gingival biopsies were collected from healthy gingival or chronic periodontitis tissue. Primary gingival keratinocytes and fibroblasts were cultured. Immunohistochemical analysis for TLR4 was performed. Transcripts of TLR4, MD-2, CD14 and LBP, and their protein products, were examined using RT-PCR, immunoprecipitation and immunoblotting. The interactions between these molecules in keratinocytes and fibroblasts were investigated by co-immunoprecipitation. RESULTS: TLR4 immunoreactivity was found in healthy gingival epithelium and periodontitis tissue, and appeared to be lower in junctional epithelium ( p
Persistent Identifierhttp://hdl.handle.net/10722/197636
ISSN
2015 Impact Factor: 2.474
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, JPen_US
dc.contributor.authorChen, Yen_US
dc.contributor.authorNg, CHCen_US
dc.contributor.authorFung, MLen_US
dc.contributor.authorXu, Aen_US
dc.contributor.authorCheng, Ben_US
dc.contributor.authorTsao, GSWen_US
dc.contributor.authorLeung, WKen_US
dc.date.accessioned2014-05-29T08:32:54Z-
dc.date.available2014-05-29T08:32:54Z-
dc.date.issued2014en_US
dc.identifier.citationJournal of Periodontal Research, 2014, v. 49 n. 6, p. 845–854en_US
dc.identifier.issn0022-3484en_US
dc.identifier.urihttp://hdl.handle.net/10722/197636-
dc.description.abstractBACKGROUND AND OBJECTIVE: Lipopolysaccharide (LPS)-mediated signaling in host cells involves Toll-like receptor 4 (TLR4) accessory molecules, including LPS-binding protein (LBP), cluster of differentiation 14 (CD14) and lymphocyte antigen 96 (MD-2). However, expression of these innate defense molecules in various compartments of the human periodontium is unclear. The aim of this study was to investigate the expression profile of TLR4 in human gingiva. MATERIAL AND METHODS: Human gingival biopsies were collected from healthy gingival or chronic periodontitis tissue. Primary gingival keratinocytes and fibroblasts were cultured. Immunohistochemical analysis for TLR4 was performed. Transcripts of TLR4, MD-2, CD14 and LBP, and their protein products, were examined using RT-PCR, immunoprecipitation and immunoblotting. The interactions between these molecules in keratinocytes and fibroblasts were investigated by co-immunoprecipitation. RESULTS: TLR4 immunoreactivity was found in healthy gingival epithelium and periodontitis tissue, and appeared to be lower in junctional epithelium ( p </= 0.01). Fibroblasts and inflammatory cells stained more strongly for TLR4 in diseased periodontal tissues (p < 0.001). Three TLR4 splicing variants, two MD-2 splicing variants and one CD14 mRNA were expressed by gingival keratinocytes and fibroblasts. Expression of TLR4, CD14 and MD-2 proteins was detected in keratinocytes and fibroblasts in vitro. TLR4 protein from gingival keratinocytes and fibroblasts could be co-immunoprecipitated with CD14 or MD-2, suggesting an association between the related molecules in vivo. LBP transcript was detected in gingival biopsies, but not in primary cultures of gingival keratinocytes or fibroblasts. CONCLUSION: TLR4, CD14 and MD-2, but not LBP, are expressed in human gingival keratinocytes and fibroblasts. The TLR4 expression level in the junctional epithelium appeared to be lowest within the periodontal epithelial barrier.en_US
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_US
dc.relation.ispartofJournal of Periodontal Researchen_US
dc.rightsThis is the accepted version of the following article: Journal of Periodontal Research, 2014, v. 49 n. 6, p. 845–854, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/jre.12173/abstracten_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleDifferential expression of Toll-like receptor 4 in healthy and diseased human gingivaen_US
dc.typeArticleen_US
dc.identifier.emailNg, CHC: hccng@hkusua.hku.hken_US
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_US
dc.identifier.emailXu, A: amxu@hkucc.hku.hken_US
dc.identifier.emailTsao, GSW: gswtsao@hku.hken_US
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hken_US
dc.identifier.authorityFung, ML=rp00433en_US
dc.identifier.authorityXu, A=rp00485en_US
dc.identifier.authorityTsao, GSW=rp00399en_US
dc.identifier.authorityLeung, WK=rp00019en_US
dc.description.naturepostprint-
dc.identifier.doi10.1111/jre.12173en_US
dc.identifier.pmid24620831en_US
dc.identifier.scopuseid_2-s2.0-84909951684-
dc.identifier.hkuros241426-
dc.identifier.hkuros252183-
dc.identifier.isiWOS:000345152300021-
dc.publisher.placeUnited Statesen_US

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