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Article: Identification of dominant pathogens in periapical lesions associated with persistent apical periodontitis

TitleIdentification of dominant pathogens in periapical lesions associated with persistent apical periodontitis
Authors
Issue Date2010
PublisherQuintessence Publishing Co Ltd. The Journal's web site is located at http://www.quintessencepublishing.co.uk/catalog/product_info.php?cPath=3&products_id=241
Citation
The Chinese Journal of Dental Research, 2010, v. 13 n. 2, p. 115-121 How to Cite?
AbstractOBJECTIVE: to identify dominant pathogens in the periapical lesions associated with persistent apical periodontitis. METHODS: thirty-three root-filled teeth with persistent apical periodontitis referred for surgical treatment were selected. Microbial samples were collected from the periapical lesions during apical surgery. Microbial identification was performed with species-specific primers prepared according to the sequence analysis data using a 16S rRNA technique. RESULTS: among the 33 cases, in 5 cases none of the target species were detected, 6 cases showed the presence of only one species, and 22 cases showed more than two species. Porphyromonas endodontalis (45% of sample) was the most commonly detected dominant microbial species in the study sample, followed by Actinomyces viscosus (42%), Candida albicans (36%) and Porphyromonas gingivalis (27%). Fusobacterium, Actinomyces israelii and Enterococcus faecalis were also detected in 27%, 21% and 15% of the sample, respectively. The most frequently isolated species, P. endodontalis, was in most cases detected together with Actinomyces (14 cases) and P. gingivalis (6 cases). None of the lesions analysed in the present study contained Prevotella intermedia. There was no correlation in relation to the presence of sinus tracts and the bacterial species. CONCLUSION: a mixed population of pathogens was found in the endodontic lesions associated with persistent apical periodontitis. P. endodontalis, A. viscosus, C. albicans and P. gingivalis were the dominant species identified.
Persistent Identifierhttp://hdl.handle.net/10722/197179
ISSN
2015 SCImago Journal Rankings: 0.272

 

DC FieldValueLanguage
dc.contributor.authorZhang, Sen_US
dc.contributor.authorWang, QQen_US
dc.contributor.authorZhang, Cen_US
dc.contributor.authorSoo, Ien_US
dc.date.accessioned2014-05-23T02:24:00Z-
dc.date.available2014-05-23T02:24:00Z-
dc.date.issued2010en_US
dc.identifier.citationThe Chinese Journal of Dental Research, 2010, v. 13 n. 2, p. 115-121en_US
dc.identifier.issn1462-6446-
dc.identifier.urihttp://hdl.handle.net/10722/197179-
dc.description.abstractOBJECTIVE: to identify dominant pathogens in the periapical lesions associated with persistent apical periodontitis. METHODS: thirty-three root-filled teeth with persistent apical periodontitis referred for surgical treatment were selected. Microbial samples were collected from the periapical lesions during apical surgery. Microbial identification was performed with species-specific primers prepared according to the sequence analysis data using a 16S rRNA technique. RESULTS: among the 33 cases, in 5 cases none of the target species were detected, 6 cases showed the presence of only one species, and 22 cases showed more than two species. Porphyromonas endodontalis (45% of sample) was the most commonly detected dominant microbial species in the study sample, followed by Actinomyces viscosus (42%), Candida albicans (36%) and Porphyromonas gingivalis (27%). Fusobacterium, Actinomyces israelii and Enterococcus faecalis were also detected in 27%, 21% and 15% of the sample, respectively. The most frequently isolated species, P. endodontalis, was in most cases detected together with Actinomyces (14 cases) and P. gingivalis (6 cases). None of the lesions analysed in the present study contained Prevotella intermedia. There was no correlation in relation to the presence of sinus tracts and the bacterial species. CONCLUSION: a mixed population of pathogens was found in the endodontic lesions associated with persistent apical periodontitis. P. endodontalis, A. viscosus, C. albicans and P. gingivalis were the dominant species identified.-
dc.languageengen_US
dc.publisherQuintessence Publishing Co Ltd. The Journal's web site is located at http://www.quintessencepublishing.co.uk/catalog/product_info.php?cPath=3&products_id=241-
dc.relation.ispartofThe Chinese Journal of Dental Researchen_US
dc.subject.meshCandida albicans - isolation and purification-
dc.subject.meshDNA, Bacterial - analysis-
dc.subject.meshDental Fistula - etiology - microbiology-
dc.subject.meshPeriapical Periodontitis - complications - microbiology - surgery-
dc.subject.meshTooth, Nonvital - microbiology - surgery-
dc.titleIdentification of dominant pathogens in periapical lesions associated with persistent apical periodontitisen_US
dc.typeArticleen_US
dc.identifier.emailZhang, C: zhangcf@hku.hken_US
dc.identifier.authorityZhang, C=rp01408en_US
dc.identifier.pmid21264361-
dc.identifier.hkuros183651en_US
dc.identifier.volume13en_US
dc.identifier.issue2-
dc.identifier.spage115en_US
dc.identifier.epage121en_US
dc.publisher.placeUnited Kingdom-

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