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Article: Microbubble-mediated sonoporation amplified lipid peroxidation of Jurkat cells

TitleMicrobubble-mediated sonoporation amplified lipid peroxidation of Jurkat cells
Authors
Issue Date2014
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/chemphyslip
Citation
Chemistry and Physics of Lipids, 2014, v. 180, p. 53-60 How to Cite?
AbstractSonoporation is a developing technique used in drug delivery for cancer cells. Low frequency ultrasound is used to trigger the cavitation of microbubbles to puncture the cell membrane, and during this process, lipid metabolism becomes disrupted. In this study, cell viability and the generation of specific oxidized lipid products were assessed in Jurkat cells before and after sonoporation. A reduction in cell viability and an induction of apoptosis of Jurkat cells were found 4h and 24h post-sonoporation, respectively. Sonoporation suppressed cholesterol concentration and arachidonic, eicosapentaenoic and docosahexaenoic acids in the Jurkat cells. Levels of enzyme-independent oxidized products (F2-isoprostanes, F3-isoprostanes, 7-ketocholesterol) were elevated by sonoporation compared with the control, whereas enzyme-dependent oxidized products (5(S)-, 9(S)-, 12(S)-, 15(S)- and 20-HETE and 27-hydroxycholesterol) were not altered. Antioxidant enzymes activities were also increased in sonoporated Jurkat cells compared with the control. In this study, the loss of lipids potentially increased the availability for enzyme-independent lipid peroxidation, leading to cell fragility and death.
Persistent Identifierhttp://hdl.handle.net/10722/196786
ISSN
2015 Impact Factor: 2.901
2015 SCImago Journal Rankings: 0.971
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLEUNG, KSen_US
dc.contributor.authorChen, Xen_US
dc.contributor.authorZhong, Wen_US
dc.contributor.authorYu, ACHen_US
dc.contributor.authorLee, CYJen_US
dc.date.accessioned2014-04-29T03:40:37Z-
dc.date.available2014-04-29T03:40:37Z-
dc.date.issued2014en_US
dc.identifier.citationChemistry and Physics of Lipids, 2014, v. 180, p. 53-60en_US
dc.identifier.issn0009-3084-
dc.identifier.urihttp://hdl.handle.net/10722/196786-
dc.description.abstractSonoporation is a developing technique used in drug delivery for cancer cells. Low frequency ultrasound is used to trigger the cavitation of microbubbles to puncture the cell membrane, and during this process, lipid metabolism becomes disrupted. In this study, cell viability and the generation of specific oxidized lipid products were assessed in Jurkat cells before and after sonoporation. A reduction in cell viability and an induction of apoptosis of Jurkat cells were found 4h and 24h post-sonoporation, respectively. Sonoporation suppressed cholesterol concentration and arachidonic, eicosapentaenoic and docosahexaenoic acids in the Jurkat cells. Levels of enzyme-independent oxidized products (F2-isoprostanes, F3-isoprostanes, 7-ketocholesterol) were elevated by sonoporation compared with the control, whereas enzyme-dependent oxidized products (5(S)-, 9(S)-, 12(S)-, 15(S)- and 20-HETE and 27-hydroxycholesterol) were not altered. Antioxidant enzymes activities were also increased in sonoporated Jurkat cells compared with the control. In this study, the loss of lipids potentially increased the availability for enzyme-independent lipid peroxidation, leading to cell fragility and death.-
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/chemphyslipen_US
dc.relation.ispartofChemistry and Physics of Lipidsen_US
dc.titleMicrobubble-mediated sonoporation amplified lipid peroxidation of Jurkat cellsen_US
dc.typeArticleen_US
dc.identifier.emailChen, X: chessy@hku.hken_US
dc.identifier.emailZhong, W: wjzhong@eee.hku.hken_US
dc.identifier.emailYu, ACH: alfred.yu@hku.hken_US
dc.identifier.emailLee, CYJ: jettylee@hku.hken_US
dc.identifier.authorityYu, ACH=rp00657en_US
dc.identifier.authorityLee, CYJ=rp01511en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chemphyslip.2014.02.004en_US
dc.identifier.pmid24569112-
dc.identifier.hkuros228735en_US
dc.identifier.hkuros228193-
dc.identifier.volume180en_US
dc.identifier.spage53en_US
dc.identifier.epage60en_US
dc.identifier.isiWOS:000335870900006-
dc.publisher.placeIreland-

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