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Article: A comparison of intravenous and intradiscal delivery of multipotential stem cells on the healing of injured intervertebral disk

TitleA comparison of intravenous and intradiscal delivery of multipotential stem cells on the healing of injured intervertebral disk
Authors
Issue Date2014
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthres
Citation
Journal of Orthopaedic Research, 2014, v. 32 n. 6, p. 819-825 How to Cite?
AbstractA major hurdle of cellular therapy for biological treatment of intervertebral disk (IVD) degeneration is the delivery method where current delivery methods are limited to intradiscal injection which can potentially cause further degeneration. Recent studies indicated that multipotential stem cells (MPSCs) from human umbilical cord blood home to injured sites and induce local therapeutic changes, thereby potentially addressing the drawbacks of direct delivery. We tested the effects of these cells on injured IVD using a mouse model of puncture-induced degeneration via two delivery methods. Caudal IVD underwent needle puncture, and MPSCs were injected indirectly (intravenously), or directly (intradiscally) into the nucleus pulposus. IVD were harvested for histological, gene and protein analysis after 14 weeks. Our finding showed limited homing ability of the MPSCs. However, regardless of delivery method, no engraftment or expansion of MPSCs was observed at the injured site. Contrasting to direct injection, intravenous injection neither improved the degeneration status, nor preserve disk height, however, both delivery methods increased glycosaminoglycan (GAG) protein and Acan gene expression relative to controls, suggesting possible paracrine effects. Identifying the mechanisms by which MPSCs act on endogenous IVD cells would provide insights into the potential of these cells to treat IVD injuries and degeneration.
Persistent Identifierhttp://hdl.handle.net/10722/196560
ISSN
2015 Impact Factor: 2.807
2015 SCImago Journal Rankings: 1.464
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTam, Ven_US
dc.contributor.authorRogers, Ien_US
dc.contributor.authorChan, Den_US
dc.contributor.authorLeung, YLVen_US
dc.contributor.authorCheung, KMCen_US
dc.date.accessioned2014-04-22T08:35:27Z-
dc.date.available2014-04-22T08:35:27Z-
dc.date.issued2014en_US
dc.identifier.citationJournal of Orthopaedic Research, 2014, v. 32 n. 6, p. 819-825en_US
dc.identifier.issn0736-0266-
dc.identifier.urihttp://hdl.handle.net/10722/196560-
dc.description.abstractA major hurdle of cellular therapy for biological treatment of intervertebral disk (IVD) degeneration is the delivery method where current delivery methods are limited to intradiscal injection which can potentially cause further degeneration. Recent studies indicated that multipotential stem cells (MPSCs) from human umbilical cord blood home to injured sites and induce local therapeutic changes, thereby potentially addressing the drawbacks of direct delivery. We tested the effects of these cells on injured IVD using a mouse model of puncture-induced degeneration via two delivery methods. Caudal IVD underwent needle puncture, and MPSCs were injected indirectly (intravenously), or directly (intradiscally) into the nucleus pulposus. IVD were harvested for histological, gene and protein analysis after 14 weeks. Our finding showed limited homing ability of the MPSCs. However, regardless of delivery method, no engraftment or expansion of MPSCs was observed at the injured site. Contrasting to direct injection, intravenous injection neither improved the degeneration status, nor preserve disk height, however, both delivery methods increased glycosaminoglycan (GAG) protein and Acan gene expression relative to controls, suggesting possible paracrine effects. Identifying the mechanisms by which MPSCs act on endogenous IVD cells would provide insights into the potential of these cells to treat IVD injuries and degeneration.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.elsevier.com/locate/orthres-
dc.relation.ispartofJournal of Orthopaedic Researchen_US
dc.rightsJournal of Orthopaedic Research. Copyright © John Wiley & Sons, Inc.-
dc.titleA comparison of intravenous and intradiscal delivery of multipotential stem cells on the healing of injured intervertebral disken_US
dc.typeArticleen_US
dc.identifier.emailTam, V: vivtam@hku.hken_US
dc.identifier.emailChan, D: chand@hku.hken_US
dc.identifier.emailLeung, YLV: vicleung@hku.hken_US
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_US
dc.identifier.authorityChan, D=rp00540en_US
dc.identifier.authorityLeung, YLV=rp01764en_US
dc.identifier.authorityCheung, KMC=rp00387en_US
dc.identifier.doi10.1002/jor.22605en_US
dc.identifier.pmid24578095-
dc.identifier.hkuros228546en_US
dc.identifier.volume32en_US
dc.identifier.issue6-
dc.identifier.spage819en_US
dc.identifier.epage825en_US
dc.identifier.isiWOS:000333716100012-
dc.publisher.placeUnited States-

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