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Article: Sonoporation-induced depolarization of plasma membrane potential: analysis of heterogeneous impact

TitleSonoporation-induced depolarization of plasma membrane potential: analysis of heterogeneous impact
Authors
Issue Date2014
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/ultrasmedbio
Citation
Ultrasound in Medicine & Biology, 2014, v. 40 n. 5, p. 979-989 How to Cite?
AbstractDisrupting plasma membrane integrity would inevitably promote anomalous ion fluxes across the membrane and thereby upset the transmembranous potential. In this article, we report new findings on how sonoporation as a physical membrane perforation strategy would lead to different forms of plasma membrane potential disruption. Our investigation was conducted with a customized fluorescence imaging platform that enabled live monitoring of plasma membrane potential in relation to individual sonoporation events triggered on HeLa cervical cancer cells. Sonovue microbubbles were used as sonoporation agents (added at 4:3 cell-to-bubble ratio), and they were activated by 1 MHz pulsed ultrasound with 0.35 MPa peak negative pressure, 20-cycle pulse duration, 20 Hz pulse repetition frequency, and 1 s total exposure duration. Results show that the plasma membrane potential response was heterogeneous among sonoporated cells: (i) membrane potential of irreversibly sonoporated cells was permanently depolarized; (ii) reversibly sonoporated cells exhibited either transient or sustained membrane depolarization; (iii) intact cells adjacent to sonoporated ones underwent transitory membrane depolarization. These findings effectively serve to substantiate the causal relationship between sonoporation and plasma membrane potential.
Persistent Identifierhttp://hdl.handle.net/10722/195915
ISSN
2015 Impact Factor: 2.298
2015 SCImago Journal Rankings: 0.885
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorQin, Pen_US
dc.contributor.authorXu, Len_US
dc.contributor.authorHu, Yen_US
dc.contributor.authorZhong, Wen_US
dc.contributor.authorCai, Pen_US
dc.contributor.authorDu, Len_US
dc.contributor.authorJin, Len_US
dc.contributor.authorYu, ACHen_US
dc.date.accessioned2014-03-21T02:19:28Z-
dc.date.available2014-03-21T02:19:28Z-
dc.date.issued2014en_US
dc.identifier.citationUltrasound in Medicine & Biology, 2014, v. 40 n. 5, p. 979-989en_US
dc.identifier.issn0301-5629en_US
dc.identifier.urihttp://hdl.handle.net/10722/195915-
dc.description.abstractDisrupting plasma membrane integrity would inevitably promote anomalous ion fluxes across the membrane and thereby upset the transmembranous potential. In this article, we report new findings on how sonoporation as a physical membrane perforation strategy would lead to different forms of plasma membrane potential disruption. Our investigation was conducted with a customized fluorescence imaging platform that enabled live monitoring of plasma membrane potential in relation to individual sonoporation events triggered on HeLa cervical cancer cells. Sonovue microbubbles were used as sonoporation agents (added at 4:3 cell-to-bubble ratio), and they were activated by 1 MHz pulsed ultrasound with 0.35 MPa peak negative pressure, 20-cycle pulse duration, 20 Hz pulse repetition frequency, and 1 s total exposure duration. Results show that the plasma membrane potential response was heterogeneous among sonoporated cells: (i) membrane potential of irreversibly sonoporated cells was permanently depolarized; (ii) reversibly sonoporated cells exhibited either transient or sustained membrane depolarization; (iii) intact cells adjacent to sonoporated ones underwent transitory membrane depolarization. These findings effectively serve to substantiate the causal relationship between sonoporation and plasma membrane potential.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/ultrasmedbioen_US
dc.relation.ispartofUltrasound in Medicine & Biologyen_US
dc.titleSonoporation-induced depolarization of plasma membrane potential: analysis of heterogeneous impacten_US
dc.typeArticleen_US
dc.identifier.emailQin, P: pqin@hku.hken_US
dc.identifier.emailZhong, W: wjzhong@eee.hku.hken_US
dc.identifier.emailYu, ACH: alfred.yu@hku.hken_US
dc.identifier.authorityYu, ACH=rp00657en_US
dc.identifier.doi10.1016/j.ultrasmedbio.2013.11.024en_US
dc.identifier.pmid24462155-
dc.identifier.hkuros228304en_US
dc.identifier.volume40-
dc.identifier.issue5-
dc.identifier.spage979-
dc.identifier.epage989-
dc.identifier.isiWOS:000333753600015-
dc.publisher.placeUnited Statesen_US

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