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Article: Dynamic localisation of mature microRNAs in Human nucleoli is influenced by exogenous genetic materials

TitleDynamic localisation of mature microRNAs in Human nucleoli is influenced by exogenous genetic materials
Authors
Issue Date2013
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2013, v. 8 n. 8, article no. e70869 How to Cite?
AbstractAlthough microRNAs are commonly known to function as a component of RNA-induced silencing complexes in the cytoplasm, they have been detected in other organelles, notably the nucleus and the nucleolus, of mammalian cells. We have conducted a systematic search for miRNAs in HeLa cell nucleoli, and identified 11 abundant miRNAs with a high level of nucleolar accumulation. Through in situ hybridisation, we have localised these miRNAs, including miR-191 and miR-484, in the nucleolus of a diversity of human and rodent cell lines. The nucleolar association of these miRNAs is resistant to various cellular stresses, but highly sensitive to the presence of exogenous nucleic acids. Introduction of both single- and double-stranded DNA as well as double stranded RNA rapidly induce the redistribution of nucleolar miRNAs to the cytoplasm. A similar change in subcellular distribution is also observed in cells infected with the influenza A virus. The partition of miRNAs between the nucleolus and the cytoplasm is affected by Leptomycin B, suggesting a role of Exportin-1 in the intracellular shuttling of miRNAs. This study reveals a previously unknown aspect of miRNA biology, and suggests a possible link between these small noncoding RNAs and the cellular management of foreign genetic materials.
Persistent Identifierhttp://hdl.handle.net/10722/195752
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, ZFen_US
dc.contributor.authorLiang, YMen_US
dc.contributor.authorLau, PNen_US
dc.contributor.authorShen, Wen_US
dc.contributor.authorWang, DKen_US
dc.contributor.authorCheung, WTen_US
dc.contributor.authorXue, CJen_US
dc.contributor.authorPoon, LLMen_US
dc.contributor.authorLam, YWen_US
dc.date.accessioned2014-03-07T04:35:11Z-
dc.date.available2014-03-07T04:35:11Z-
dc.date.issued2013en_US
dc.identifier.citationPLoS One, 2013, v. 8 n. 8, article no. e70869en_US
dc.identifier.urihttp://hdl.handle.net/10722/195752-
dc.description.abstractAlthough microRNAs are commonly known to function as a component of RNA-induced silencing complexes in the cytoplasm, they have been detected in other organelles, notably the nucleus and the nucleolus, of mammalian cells. We have conducted a systematic search for miRNAs in HeLa cell nucleoli, and identified 11 abundant miRNAs with a high level of nucleolar accumulation. Through in situ hybridisation, we have localised these miRNAs, including miR-191 and miR-484, in the nucleolus of a diversity of human and rodent cell lines. The nucleolar association of these miRNAs is resistant to various cellular stresses, but highly sensitive to the presence of exogenous nucleic acids. Introduction of both single- and double-stranded DNA as well as double stranded RNA rapidly induce the redistribution of nucleolar miRNAs to the cytoplasm. A similar change in subcellular distribution is also observed in cells infected with the influenza A virus. The partition of miRNAs between the nucleolus and the cytoplasm is affected by Leptomycin B, suggesting a role of Exportin-1 in the intracellular shuttling of miRNAs. This study reveals a previously unknown aspect of miRNA biology, and suggests a possible link between these small noncoding RNAs and the cellular management of foreign genetic materials.en_US
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleDynamic localisation of mature microRNAs in Human nucleoli is influenced by exogenous genetic materialsen_US
dc.typeArticleen_US
dc.identifier.emailLau, PN: sbsylvia@hku.hken_US
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_US
dc.identifier.authorityPoon, LLM=rp00484en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0070869en_US
dc.identifier.pmid23940654-
dc.identifier.hkuros228167en_US
dc.identifier.volume8en_US
dc.identifier.issue8en_US
dc.identifier.isiWOS:000324401500052-

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