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Article: Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis

TitleIntrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis
Authors
Issue Date2010
Citation
Kidney International, 2010, v. 78 n. 12, p. 1263-1274 How to Cite?
AbstractAntimyeloperoxidase antibodies can cause crescentic glomerulonephritis and pulmonary hemorrhage. Toll-like receptors (TLRs) respond to infectious agents activating host defenses, whereas infections potentially initiate disease and provoke relapses. Neutrophils were found to be key effector cells of injury in experimental models, as disease does not occur in their absence and injury is enhanced by lipopolysaccharide (LPS). In this study, highly purified LPS (a pure TLR4 ligand) acted with antimyeloperoxidase antibodies to synergistically increase kidney and lung neutrophil recruitment and functional injury; effects abrogated in TLR4-deficient mice. Increased kidney TLR4 expression after stimulation predominantly occurred in glomerular endothelial cells. Enhanced glomerular neutrophil recruitment correlated with increased kidney mRNA expression of CXCL1 and CXCL2 (homologs of human CXCL8), whereas their preemptive neutralization decreased neutrophil recruitment. Disease induction in bone marrow chimeric mice showed that TLR4 in both bone marrow and renal parenchymal cells is required for maximal neutrophil recruitment and glomerular injury. Further studies in human glomerular cell lines stimulated with LPS found that glomerular endothelial cells were the prominent sources of CXCL8. Thus, our results define a role for TLR4 expression in bone marrow-derived and glomerular endothelial cells in neutrophil recruitment and subsequent functional and histological renal injury in experimental antimyeloperoxidase glomerulonephritis. © 2010 International Society of Nephrology.
Persistent Identifierhttp://hdl.handle.net/10722/195492
ISSN
2015 Impact Factor: 7.683
2015 SCImago Journal Rankings: 3.181
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSummers, SA-
dc.contributor.authorVan Der Veen, BS-
dc.contributor.authorO'Sullivan, KM-
dc.contributor.authorGan, P-Y-
dc.contributor.authorOoi, JD-
dc.contributor.authorHeeringa, P-
dc.contributor.authorSatchell, SC-
dc.contributor.authorMathieson, PW-
dc.contributor.authorSaleem, MA-
dc.contributor.authorVisvanathan, K-
dc.contributor.authorHoldsworth, SR-
dc.contributor.authorKitching, AR-
dc.date.accessioned2014-02-28T06:12:14Z-
dc.date.available2014-02-28T06:12:14Z-
dc.date.issued2010-
dc.identifier.citationKidney International, 2010, v. 78 n. 12, p. 1263-1274-
dc.identifier.issn0085-2538-
dc.identifier.urihttp://hdl.handle.net/10722/195492-
dc.description.abstractAntimyeloperoxidase antibodies can cause crescentic glomerulonephritis and pulmonary hemorrhage. Toll-like receptors (TLRs) respond to infectious agents activating host defenses, whereas infections potentially initiate disease and provoke relapses. Neutrophils were found to be key effector cells of injury in experimental models, as disease does not occur in their absence and injury is enhanced by lipopolysaccharide (LPS). In this study, highly purified LPS (a pure TLR4 ligand) acted with antimyeloperoxidase antibodies to synergistically increase kidney and lung neutrophil recruitment and functional injury; effects abrogated in TLR4-deficient mice. Increased kidney TLR4 expression after stimulation predominantly occurred in glomerular endothelial cells. Enhanced glomerular neutrophil recruitment correlated with increased kidney mRNA expression of CXCL1 and CXCL2 (homologs of human CXCL8), whereas their preemptive neutralization decreased neutrophil recruitment. Disease induction in bone marrow chimeric mice showed that TLR4 in both bone marrow and renal parenchymal cells is required for maximal neutrophil recruitment and glomerular injury. Further studies in human glomerular cell lines stimulated with LPS found that glomerular endothelial cells were the prominent sources of CXCL8. Thus, our results define a role for TLR4 expression in bone marrow-derived and glomerular endothelial cells in neutrophil recruitment and subsequent functional and histological renal injury in experimental antimyeloperoxidase glomerulonephritis. © 2010 International Society of Nephrology.-
dc.languageeng-
dc.relation.ispartofKidney International-
dc.titleIntrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/ki.2010.327-
dc.identifier.pmid20844472-
dc.identifier.scopuseid_2-s2.0-78649667341-
dc.identifier.volume78-
dc.identifier.issue12-
dc.identifier.spage1263-
dc.identifier.epage1274-
dc.identifier.isiWOS:000284752900010-

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