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Article: Novel conditionally immortalized human proximal tubule cell line expressing functional influx and efflux transporters

TitleNovel conditionally immortalized human proximal tubule cell line expressing functional influx and efflux transporters
Authors
Issue Date2010
Citation
Cell and Tissue Research, 2010, v. 339 n. 2, p. 449-457 How to Cite?
AbstractReabsorption of filtered solutes from the glomerular filtrate and excretion of waste products and xenobiotics are the main functions of the renal proximal tubular (PT) epithelium. A human PT cell line expressing a range of functional transporters would help to augment current knowledge in renal physiology and pharmacology. We have established and characterized a conditionally immortalized PT epithelial cell line (ciPTEC) obtained by transfecting and subcloning cells exfoliated in the urine of a healthy volunteer. The PT origin of this line has been confirmed morphologically and by the expression of aminopeptidase N, zona occludens 1, aquaporin 1, dipeptidyl peptidase IV and multidrug resistance protein 4 together with alkaline phosphatase activity. ciPTEC assembles in a tight monolayer with limited diffusion of inulin-fluorescein-isothiocyanate. Concentration and time-dependent reabsorption of albumin via endocytosis has been demonstrated, together with sodium-dependent phosphate uptake. The expression and activity of apical efflux transporter p-glycoprotein and of baso-lateral influx transporter organic cation transporter 2 have been shown in ciPTEC. This established human ciPTEC expressing multiple endogenous organic ion transporters mimicking renal reabsorption and excretion represents a powerful tool for future in vitro transport studies in pharmacology and physiology.
Persistent Identifierhttp://hdl.handle.net/10722/195484
ISSN
2015 Impact Factor: 2.948
2015 SCImago Journal Rankings: 1.539
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWilmer, MJ-
dc.contributor.authorSaleem, MA-
dc.contributor.authorMasereeuw, R-
dc.contributor.authorNi, L-
dc.contributor.authorVan Der Velden, TJ-
dc.contributor.authorRussel, FG-
dc.contributor.authorMathieson, PW-
dc.contributor.authorMonnens, LA-
dc.contributor.authorVan Den Heuvel, LP-
dc.contributor.authorLevtchenko, EN-
dc.date.accessioned2014-02-28T06:12:13Z-
dc.date.available2014-02-28T06:12:13Z-
dc.date.issued2010-
dc.identifier.citationCell and Tissue Research, 2010, v. 339 n. 2, p. 449-457-
dc.identifier.issn0302-766X-
dc.identifier.urihttp://hdl.handle.net/10722/195484-
dc.description.abstractReabsorption of filtered solutes from the glomerular filtrate and excretion of waste products and xenobiotics are the main functions of the renal proximal tubular (PT) epithelium. A human PT cell line expressing a range of functional transporters would help to augment current knowledge in renal physiology and pharmacology. We have established and characterized a conditionally immortalized PT epithelial cell line (ciPTEC) obtained by transfecting and subcloning cells exfoliated in the urine of a healthy volunteer. The PT origin of this line has been confirmed morphologically and by the expression of aminopeptidase N, zona occludens 1, aquaporin 1, dipeptidyl peptidase IV and multidrug resistance protein 4 together with alkaline phosphatase activity. ciPTEC assembles in a tight monolayer with limited diffusion of inulin-fluorescein-isothiocyanate. Concentration and time-dependent reabsorption of albumin via endocytosis has been demonstrated, together with sodium-dependent phosphate uptake. The expression and activity of apical efflux transporter p-glycoprotein and of baso-lateral influx transporter organic cation transporter 2 have been shown in ciPTEC. This established human ciPTEC expressing multiple endogenous organic ion transporters mimicking renal reabsorption and excretion represents a powerful tool for future in vitro transport studies in pharmacology and physiology.-
dc.languageeng-
dc.relation.ispartofCell and Tissue Research-
dc.titleNovel conditionally immortalized human proximal tubule cell line expressing functional influx and efflux transporters-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00441-009-0882-y-
dc.identifier.pmid19902259-
dc.identifier.scopuseid_2-s2.0-77949269535-
dc.identifier.volume339-
dc.identifier.issue2-
dc.identifier.spage449-
dc.identifier.epage457-
dc.identifier.isiWOS:000274331000014-

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