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Article: A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression

TitleA conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression
Authors
Issue Date2002
Citation
Journal of the American Society of Nephrology, 2002, v. 13 n. 3, p. 630-638 How to Cite?
AbstractRecent molecular insights have established the podocyte as a key component of the glomerular filtration barrier, and hence an important common pathway in proteinuric diseases. A conditionally immortalized human podocyte cell line has been developed by transfection with the temperature-sensitive SV40-T gene. These cells proliferate at the "permissive" temperature (33°C). After transfer to the "nonpermissive" temperature (37°C), they entered growth arrest and expressed markers of differentiated in vivo podocytes, including the novel podocyte proteins, nephrin, podocin, CD2AP, and synaptopodin, and known molecules of the slit diaphragm ZO-1, α-, β-,and γ-catenin and P-cadherin. The differentiation was accompanied by a growth arrest and the upregulation of cyclin-dependent kinase inhibitors, p27 and p57, as well as cyclin D1, whereas cyclin A was downregulated. These data are consistent with cell cycle protein expression during podocyte maturation in vivo. In conclusion, the development of this cell line provides a new tool in the study of podocyte biology, which will enable accurate assessment of the behavior of these complex cells in health and disease.
Persistent Identifierhttp://hdl.handle.net/10722/195366
ISSN
2015 Impact Factor: 8.491
2015 SCImago Journal Rankings: 4.699
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSaleem, MA-
dc.contributor.authorO'Hare, MJ-
dc.contributor.authorReiser, J-
dc.contributor.authorCoward, RJ-
dc.contributor.authorInward, CD-
dc.contributor.authorFarren, T-
dc.contributor.authorChang, YX-
dc.contributor.authorNi, L-
dc.contributor.authorMathieson, PW-
dc.contributor.authorMundel, P-
dc.date.accessioned2014-02-28T06:12:02Z-
dc.date.available2014-02-28T06:12:02Z-
dc.date.issued2002-
dc.identifier.citationJournal of the American Society of Nephrology, 2002, v. 13 n. 3, p. 630-638-
dc.identifier.issn1046-6673-
dc.identifier.urihttp://hdl.handle.net/10722/195366-
dc.description.abstractRecent molecular insights have established the podocyte as a key component of the glomerular filtration barrier, and hence an important common pathway in proteinuric diseases. A conditionally immortalized human podocyte cell line has been developed by transfection with the temperature-sensitive SV40-T gene. These cells proliferate at the "permissive" temperature (33°C). After transfer to the "nonpermissive" temperature (37°C), they entered growth arrest and expressed markers of differentiated in vivo podocytes, including the novel podocyte proteins, nephrin, podocin, CD2AP, and synaptopodin, and known molecules of the slit diaphragm ZO-1, α-, β-,and γ-catenin and P-cadherin. The differentiation was accompanied by a growth arrest and the upregulation of cyclin-dependent kinase inhibitors, p27 and p57, as well as cyclin D1, whereas cyclin A was downregulated. These data are consistent with cell cycle protein expression during podocyte maturation in vivo. In conclusion, the development of this cell line provides a new tool in the study of podocyte biology, which will enable accurate assessment of the behavior of these complex cells in health and disease.-
dc.languageeng-
dc.relation.ispartofJournal of the American Society of Nephrology-
dc.titleA conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11856766-
dc.identifier.scopuseid_2-s2.0-0036189998-
dc.identifier.volume13-
dc.identifier.issue3-
dc.identifier.spage630-
dc.identifier.epage638-
dc.identifier.isiWOS:000173964000007-

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