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Article: Intercellular channels in teleosts: functional characterization of two connexins from Atlantic croaker

TitleIntercellular channels in teleosts: functional characterization of two connexins from Atlantic croaker
Authors
Issue Date1995
PublisherElsevier BV.
Citation
FEBS Letters, 1995, v. 358 n. 3, p. 301-304 How to Cite?
AbstractGap junction channels, composed of protein subunits termed connexins, are believed to play a critical role in the process of oocyte differentiation and maturation. We have used the paired Xenopus oocyte assay to characterize functionally two connexin genes, connexin-32.2 and connexin-32.7, recently cloned from the ovary of the Atlantic croaker (Micropogonia undulatus), a species that has emerged as a useful model to study the process of maturation of the ovarian follicle. We have found that, while both connexin proteins were expressed at comparable levels in Xenopus oocytes, only one, connexin-32.2, was functionally competent to induce the formation of intercellular channels. Connexin-32.2 channels exhibited voltage-dependent closure that was similar to, but distinct from that of previously characterized mammalian connexins. In addition, the silent connexin-32.7 was unable to functionally interact with connexin-32.2, either in heterotypic channels or as dominant negative inhibitor. Because connexin-32.2 expression is strikingly regulated during oocyte maturation, these data provide further evidence for a role of intercellular channels in the control of oocyte-follicular cell interactions.
Persistent Identifierhttp://hdl.handle.net/10722/195295
ISSN
2015 Impact Factor: 3.519
2015 SCImago Journal Rankings: 2.026
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBruzzone, R-
dc.contributor.authorWhite, TW-
dc.contributor.authorYoshizaki, G-
dc.contributor.authorPatiño, R-
dc.contributor.authorPaul, DL-
dc.date.accessioned2014-02-26T06:21:48Z-
dc.date.available2014-02-26T06:21:48Z-
dc.date.issued1995-
dc.identifier.citationFEBS Letters, 1995, v. 358 n. 3, p. 301-304-
dc.identifier.issn0014-5793-
dc.identifier.urihttp://hdl.handle.net/10722/195295-
dc.description.abstractGap junction channels, composed of protein subunits termed connexins, are believed to play a critical role in the process of oocyte differentiation and maturation. We have used the paired Xenopus oocyte assay to characterize functionally two connexin genes, connexin-32.2 and connexin-32.7, recently cloned from the ovary of the Atlantic croaker (Micropogonia undulatus), a species that has emerged as a useful model to study the process of maturation of the ovarian follicle. We have found that, while both connexin proteins were expressed at comparable levels in Xenopus oocytes, only one, connexin-32.2, was functionally competent to induce the formation of intercellular channels. Connexin-32.2 channels exhibited voltage-dependent closure that was similar to, but distinct from that of previously characterized mammalian connexins. In addition, the silent connexin-32.7 was unable to functionally interact with connexin-32.2, either in heterotypic channels or as dominant negative inhibitor. Because connexin-32.2 expression is strikingly regulated during oocyte maturation, these data provide further evidence for a role of intercellular channels in the control of oocyte-follicular cell interactions.-
dc.languageeng-
dc.publisherElsevier BV.-
dc.relation.ispartofFEBS Letters-
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in FEBS Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in FEBS Letters, [VOL 358, ISSUE 3, 1995] DOI 10.1016/0014-5793(94)01457-C-
dc.subject.meshConnexins - genetics - metabolism - physiology-
dc.subject.meshIon Channels - genetics - metabolism - physiology-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPerciformes - metabolism-
dc.subject.meshXenopus laevis-
dc.titleIntercellular channels in teleosts: functional characterization of two connexins from Atlantic croakeren_US
dc.typeArticleen_US
dc.identifier.emailBruzzone, R: bruzzone@HKUCC.hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0014-5793(94)01457-C-
dc.identifier.pmid7531161-
dc.identifier.volume358-
dc.identifier.issue3-
dc.identifier.spage301-
dc.identifier.epage304-
dc.identifier.isiWOS:A1995QE77400019-
dc.publisher.placeNetherlands-

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