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Article: A distant upstream region of the rat multipartite Na+-Ca2+ exchanger NCX1 gene promoter is sufficient to confer cardiac-specific expression

TitleA distant upstream region of the rat multipartite Na+-Ca2+ exchanger NCX1 gene promoter is sufficient to confer cardiac-specific expression
Authors
Issue Date2001
Citation
Mechanisms of Development, 2001, v. 109 n. 2, p. 267-279 How to Cite?
AbstractThe Na+-Ca2+ exchanger (NCX) regulates intracellular calcium homeostasis. We report on an upstream region of the rat NCX1 multipartite promoter that is active in cardiac myocytes. Although inactive in most non-cardiac cell lines, its activity can be rescued by cotransfection with GATA-4 and -6, but not GATA-5 transcription factors. In transgenic mice and similar to endogenous NCX1 mRNA expression, the upstream promoter region directs uniform β-galactosidase expression in cardiac myocytes from ∼7.75 dpc. In adult mouse hearts, promoter activity is, however, significantly reduced and heterogeneous, except in the conduction system (sinoatrial and atrioventricular node, atrioventricular bundles). The upstream NCX1 promoter region thus directs appropriate spatial and temporal control of cardiac expression throughout development.
Persistent Identifierhttp://hdl.handle.net/10722/195252
ISSN
2015 Impact Factor: 2.041
2015 SCImago Journal Rankings: 1.368
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKoban, MU-
dc.contributor.authorBrugh, SA-
dc.contributor.authorRiordon, DR-
dc.contributor.authorDellow, KA-
dc.contributor.authorYang, H-T-
dc.contributor.authorTweedie, D-
dc.contributor.authorBoheler, KR-
dc.date.accessioned2014-02-25T01:40:21Z-
dc.date.available2014-02-25T01:40:21Z-
dc.date.issued2001-
dc.identifier.citationMechanisms of Development, 2001, v. 109 n. 2, p. 267-279-
dc.identifier.issn0925-4773-
dc.identifier.urihttp://hdl.handle.net/10722/195252-
dc.description.abstractThe Na+-Ca2+ exchanger (NCX) regulates intracellular calcium homeostasis. We report on an upstream region of the rat NCX1 multipartite promoter that is active in cardiac myocytes. Although inactive in most non-cardiac cell lines, its activity can be rescued by cotransfection with GATA-4 and -6, but not GATA-5 transcription factors. In transgenic mice and similar to endogenous NCX1 mRNA expression, the upstream promoter region directs uniform β-galactosidase expression in cardiac myocytes from ∼7.75 dpc. In adult mouse hearts, promoter activity is, however, significantly reduced and heterogeneous, except in the conduction system (sinoatrial and atrioventricular node, atrioventricular bundles). The upstream NCX1 promoter region thus directs appropriate spatial and temporal control of cardiac expression throughout development.-
dc.languageeng-
dc.relation.ispartofMechanisms of Development-
dc.titleA distant upstream region of the rat multipartite Na+-Ca2+ exchanger NCX1 gene promoter is sufficient to confer cardiac-specific expression-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0925-4773(01)00548-2-
dc.identifier.pmid11731239-
dc.identifier.scopuseid_2-s2.0-0035193555-
dc.identifier.volume109-
dc.identifier.issue2-
dc.identifier.spage267-
dc.identifier.epage279-
dc.identifier.isiWOS:000172737400013-

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