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Article: Two-dimensional gel electrophoresis database of cultured murine R1 embryonic stem cells

TitleTwo-dimensional gel electrophoresis database of cultured murine R1 embryonic stem cells
Authors
Issue Date2004
Citation
Proteomics, 2004, v. 4 n. 12, p. 3813-3832 How to Cite?
AbstractEmbryonic stem (ES) cell lines represent a population of undifferentiated pluripotent cells capable of multilineage differentiation in vitro. Although very useful for studying developmental processes, human ES cell lines have also been suggested as a potential and unlimited source for cellular transplantation and the treatment of human disease. The proteomic basis of embryonic stemness (pluripotentiality and multilineage differentiation) and the transitions that lead to specific cell lineages however, remain to be defined. As an important first step in defining these processes, we have performed a proteomic analysis of undifferentiated mouse R1 ES cell lines using pH 3-10, 4-7 and 6-11 two-dimensional electrophoresis gels, matrix-assisted laser desorption/ ionization and tandem mass spectrometry. Of the 700 gel spots analyzed, 241 distinct protein species were identified corresponding to 218 unique proteins, with a significant proportion functionally related to protein expression.
Persistent Identifierhttp://hdl.handle.net/10722/195171
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorElliott, ST-
dc.contributor.authorCrider, DG-
dc.contributor.authorGarham, CP-
dc.contributor.authorBoheler, KR-
dc.contributor.authorVan Eyk, JE-
dc.date.accessioned2014-02-25T01:40:16Z-
dc.date.available2014-02-25T01:40:16Z-
dc.date.issued2004-
dc.identifier.citationProteomics, 2004, v. 4 n. 12, p. 3813-3832-
dc.identifier.issn1615-9853-
dc.identifier.urihttp://hdl.handle.net/10722/195171-
dc.description.abstractEmbryonic stem (ES) cell lines represent a population of undifferentiated pluripotent cells capable of multilineage differentiation in vitro. Although very useful for studying developmental processes, human ES cell lines have also been suggested as a potential and unlimited source for cellular transplantation and the treatment of human disease. The proteomic basis of embryonic stemness (pluripotentiality and multilineage differentiation) and the transitions that lead to specific cell lineages however, remain to be defined. As an important first step in defining these processes, we have performed a proteomic analysis of undifferentiated mouse R1 ES cell lines using pH 3-10, 4-7 and 6-11 two-dimensional electrophoresis gels, matrix-assisted laser desorption/ ionization and tandem mass spectrometry. Of the 700 gel spots analyzed, 241 distinct protein species were identified corresponding to 218 unique proteins, with a significant proportion functionally related to protein expression.-
dc.languageeng-
dc.relation.ispartofProteomics-
dc.titleTwo-dimensional gel electrophoresis database of cultured murine R1 embryonic stem cells-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.200300820-
dc.identifier.pmid15378706-
dc.identifier.scopuseid_2-s2.0-10644237907-
dc.identifier.volume4-
dc.identifier.issue12-
dc.identifier.spage3813-
dc.identifier.epage3832-
dc.identifier.isiWOS:000225801200013-

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