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Article: Differential response of cardiac fibroblasts from young adult and senescent rats to ANG II

TitleDifferential response of cardiac fibroblasts from young adult and senescent rats to ANG II
Authors
Issue Date2003
Citation
American Journal of Physiology - Heart and Circulatory Physiology, 2003, v. 284 n. 4 53-4, p. H1454-H1459 How to Cite?
AbstractThe intracardiac ANG II-forming pathway is activated in the senescent myocardium, raising the possibility of enhanced ANG II effects on cardiac fibroblasts. This study established an in vitro model of cultured cardiac fibroblasts from aged rats to examine if the response of these cells to ANG II is modified in the aged heart. Levels of mRNA encoding renin, angiotensinogen, and the AT1 receptor subtype in cardiac fibroblasts from young adult and senescent rats were quantified by RT-PCR, net collagen production by a hydroxyproline-based assay, and transforming growth factor (TGF)-β levels using a commercial kit. In cardiac fibroblasts from young adult rats, ANG II significantly enhanced AT1 mRNA levels, net collagen production, and TGF-β production. In fibroblasts from the aged myocardium, ANG II downregulated AT1 mRNA expression, had a less pronounced effect on net collagen production, and had no effect on TGF-β production. Such age-related modification of the response of cardiac fibroblasts to ANG II may counteract the effects of augmented intracardiac ANG II production in the senescent heart, limiting fibrogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/195165
ISSN
2015 Impact Factor: 3.324
2015 SCImago Journal Rankings: 1.823
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShivakumar, K-
dc.contributor.authorDostal, DE-
dc.contributor.authorBoheler, K-
dc.contributor.authorBaker, KM-
dc.contributor.authorLakatta, EG-
dc.date.accessioned2014-02-25T01:40:15Z-
dc.date.available2014-02-25T01:40:15Z-
dc.date.issued2003-
dc.identifier.citationAmerican Journal of Physiology - Heart and Circulatory Physiology, 2003, v. 284 n. 4 53-4, p. H1454-H1459-
dc.identifier.issn0363-6135-
dc.identifier.urihttp://hdl.handle.net/10722/195165-
dc.description.abstractThe intracardiac ANG II-forming pathway is activated in the senescent myocardium, raising the possibility of enhanced ANG II effects on cardiac fibroblasts. This study established an in vitro model of cultured cardiac fibroblasts from aged rats to examine if the response of these cells to ANG II is modified in the aged heart. Levels of mRNA encoding renin, angiotensinogen, and the AT1 receptor subtype in cardiac fibroblasts from young adult and senescent rats were quantified by RT-PCR, net collagen production by a hydroxyproline-based assay, and transforming growth factor (TGF)-β levels using a commercial kit. In cardiac fibroblasts from young adult rats, ANG II significantly enhanced AT1 mRNA levels, net collagen production, and TGF-β production. In fibroblasts from the aged myocardium, ANG II downregulated AT1 mRNA expression, had a less pronounced effect on net collagen production, and had no effect on TGF-β production. Such age-related modification of the response of cardiac fibroblasts to ANG II may counteract the effects of augmented intracardiac ANG II production in the senescent heart, limiting fibrogenesis.-
dc.languageeng-
dc.relation.ispartofAmerican Journal of Physiology - Heart and Circulatory Physiology-
dc.titleDifferential response of cardiac fibroblasts from young adult and senescent rats to ANG II-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1152/ajpheart.00766.2002-
dc.identifier.pmid12595286-
dc.identifier.scopuseid_2-s2.0-0037377574-
dc.identifier.volume284-
dc.identifier.issue4 53-4-
dc.identifier.spageH1454-
dc.identifier.epageH1459-
dc.identifier.isiWOS:000181425900048-

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