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Article: Expanding the mouse embryonic stem cell proteome: Combining three proteomic approaches

TitleExpanding the mouse embryonic stem cell proteome: Combining three proteomic approaches
Authors
Issue Date2010
Citation
Proteomics, 2010, v. 10 n. 14, p. 2728-2732 How to Cite?
AbstractThe current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/195127
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGundry, RL-
dc.contributor.authorTchernyshyov, I-
dc.contributor.authorSheng, S-
dc.contributor.authorTarasova, Y-
dc.contributor.authorRaginski, K-
dc.contributor.authorBoheler, KR-
dc.contributor.authorVan Eyk, JE-
dc.date.accessioned2014-02-25T01:40:12Z-
dc.date.available2014-02-25T01:40:12Z-
dc.date.issued2010-
dc.identifier.citationProteomics, 2010, v. 10 n. 14, p. 2728-2732-
dc.identifier.issn1615-9853-
dc.identifier.urihttp://hdl.handle.net/10722/195127-
dc.description.abstractThe current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.-
dc.languageeng-
dc.relation.ispartofProteomics-
dc.titleExpanding the mouse embryonic stem cell proteome: Combining three proteomic approaches-
dc.typeArticle-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.201000039-
dc.identifier.pmid20512790-
dc.identifier.scopuseid_2-s2.0-77954732858-
dc.identifier.volume10-
dc.identifier.issue14-
dc.identifier.spage2728-
dc.identifier.epage2732-
dc.identifier.isiWOS:000280615200015-

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