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Article: Nerve growth factor receptors in rat spinal cord: An autoradiographic and immunohistochemical study

TitleNerve growth factor receptors in rat spinal cord: An autoradiographic and immunohistochemical study
Authors
Issue Date1987
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 1987, v. 22 n. 1, p. 267-279 How to Cite?
AbstractThe distribution of nerve growth factor receptors in the lumbar spinal cord of the rat was studied with autoradiographic and immunohistochemical techniques: [125I]nerve growth factor and specific monoclonal antibody (Mab 192) against nerve growth factor receptor were used to localize nerve growth factor binding sites. The distributions of nerve growth factor binding sites with highest density within the superficial layers (laminae I and II) of the dorsal gray matter were virtually identical as demonstrated by these two ligands; this suggests that Mab 192 can be used as a specific probe to identify nerve growth factor receptors in rat nervous system. Nerve growth factor receptor binding sites, as demonstrated by autoradiography, were also found in longitudinal bundles of fibers running dorsolaterally in the lateral funiculus. However, no immunoreactivity was detected in these areas by immunohistochemistry. No specific binding was found in the dorsal horn when [125I]nerve growth factor was co-injected with unlabeled nerve growth factor or after incubation with nonspecific monoclonal antibody. Dorsal root section produced a complete loss of nerve growth factor-specific labeling pattern throughout laminae I-II of the spinal cord. This suggests that nerve growth factor receptors are localized on the nerve terminals of primary afferent fibers which synapse in the region of the spinal cord. The presence of nerve growth factor binding sites in the dorsal horn of the spinal cord is consistent with the possibility that nerve growth factor, or a nerve growth factor-like substance, derived from the central nervous system, may have a role in trophic support of dorsal root ganglion neurons.
Persistent Identifierhttp://hdl.handle.net/10722/194888
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYip, HKF-
dc.contributor.authorJohnson, EM Jr-
dc.date.accessioned2014-02-17T06:48:03Z-
dc.date.available2014-02-17T06:48:03Z-
dc.date.issued1987-
dc.identifier.citationNeuroscience, 1987, v. 22 n. 1, p. 267-279-
dc.identifier.issn0306-4522-
dc.identifier.urihttp://hdl.handle.net/10722/194888-
dc.description.abstractThe distribution of nerve growth factor receptors in the lumbar spinal cord of the rat was studied with autoradiographic and immunohistochemical techniques: [125I]nerve growth factor and specific monoclonal antibody (Mab 192) against nerve growth factor receptor were used to localize nerve growth factor binding sites. The distributions of nerve growth factor binding sites with highest density within the superficial layers (laminae I and II) of the dorsal gray matter were virtually identical as demonstrated by these two ligands; this suggests that Mab 192 can be used as a specific probe to identify nerve growth factor receptors in rat nervous system. Nerve growth factor receptor binding sites, as demonstrated by autoradiography, were also found in longitudinal bundles of fibers running dorsolaterally in the lateral funiculus. However, no immunoreactivity was detected in these areas by immunohistochemistry. No specific binding was found in the dorsal horn when [125I]nerve growth factor was co-injected with unlabeled nerve growth factor or after incubation with nonspecific monoclonal antibody. Dorsal root section produced a complete loss of nerve growth factor-specific labeling pattern throughout laminae I-II of the spinal cord. This suggests that nerve growth factor receptors are localized on the nerve terminals of primary afferent fibers which synapse in the region of the spinal cord. The presence of nerve growth factor binding sites in the dorsal horn of the spinal cord is consistent with the possibility that nerve growth factor, or a nerve growth factor-like substance, derived from the central nervous system, may have a role in trophic support of dorsal root ganglion neurons.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience-
dc.relation.ispartofNeuroscience-
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Neuroscience. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Neuroscience, [VOL 22, ISSUE , 1987]-
dc.subject.meshAfferent Pathways - anatomy and histology-
dc.subject.meshNerve Growth Factors - metabolism-
dc.subject.meshReceptors, Cell Surface - metabolism-
dc.subject.meshReceptors, Nerve Growth Factor-
dc.subject.meshSpinal Cord - anatomy and histology-
dc.titleNerve growth factor receptors in rat spinal cord: An autoradiographic and immunohistochemical studyen_US
dc.typeArticleen_US
dc.identifier.emailYip, HKF: hkfyip@hku.hk, hkfyip@hkusua.hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0306-4522(87)90217-X-
dc.identifier.pmid2819775-
dc.identifier.scopuseid_2-s2.0-0023274471-
dc.identifier.volume22-
dc.identifier.issue1-
dc.identifier.spage267-
dc.identifier.epage279-
dc.identifier.isiWOS:A1987J271600026-
dc.publisher.placeNetherlands-

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