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Article: Infection of swine ex vivo tissues with avian viruses including H7N9 and correlation with glycomic analysis

TitleInfection of swine ex vivo tissues with avian viruses including H7N9 and correlation with glycomic analysis
Authors
Issue Date2013
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/subs.asp?ref=1750-2640&site=1
Citation
Influenza and Other Respiratory Viruses, 2013, v. 7 n. 6, p. 1269-1282 How to Cite?
AbstractOBJECTIVES: Swine have been regarded as intermediate hosts in the spread of influenza from birds to humans but studies of the sialylated glycans that comprise their respiratory tract have not been extensively studied in the past. This study analyzed the sialylated N-glycan and O-glycan profile of swine trachea and lung and correlated this with ex-vivo infection of swine explants with avian influenza viruses. SAMPLE: Lungs and tracheal samples were obtained from normal farm and laboratory raised swine and used for ex vivo infection as well as mass spectrometric analysis. Infection of the ex vivo tissues used high pathogenic and low pathogenic avian viruses including the novel H7N9 virus that emerged in China in early 2013. MAIN OUTCOME MEASURES: Assessment of successful replication was determined by TCID50 as well as virus immunohistochemistry. The N-glycan and O-glycan profiles were measured by MALDI-TOF and sialylated linkages were determined by sialidase treatment. Lectin binding histochemistry was also performed on formalin fixed tissue samples with positive binding detected by chromogen staining. RESULTS: The swine respiratory tract glycans differed from the human respiratory tact glycans in two main areas. There was a greater abundance of Gal-α-Gal linkages resulting in a relative decrease in sialylated glycans. The swine respiratory tract also had a greater proportion of glycans containing Neu5Gc and Siaα2-6 glycans than the human respiratory tract. Infection with avian viruses was confined primarily to lung bronchioles rather than trachea and parenchyma. CONCLUSIONS: In contrast to previous studies we found that there was not as much expression of Siaα2-3 glycans on the surface of the trachea. Infection of Siaα2-3 binding avian viruses was restricted to the lower respiratory tract bronchioles. This finding may diminish the ability of the swine to act as an intermediary in the transmission of avian viruses to humans.
Persistent Identifierhttp://hdl.handle.net/10722/194837
ISSN
2015 Impact Factor: 2.378
2015 SCImago Journal Rankings: 1.570
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, WYen_US
dc.contributor.authorKaramanska, Ren_US
dc.contributor.authorVan Poucke, Sen_US
dc.contributor.authorVan Reeth, Ken_US
dc.contributor.authorChan, WWen_US
dc.contributor.authorChan, MCWen_US
dc.contributor.authorDell, Aen_US
dc.contributor.authorPeiris, JSMen_US
dc.contributor.authorHaslam, SMen_US
dc.contributor.authorGuan, Yen_US
dc.contributor.authorNicholls, JMen_US
dc.date.accessioned2014-02-17T02:14:42Z-
dc.date.available2014-02-17T02:14:42Z-
dc.date.issued2013en_US
dc.identifier.citationInfluenza and Other Respiratory Viruses, 2013, v. 7 n. 6, p. 1269-1282en_US
dc.identifier.issn1750-2640-
dc.identifier.urihttp://hdl.handle.net/10722/194837-
dc.description.abstractOBJECTIVES: Swine have been regarded as intermediate hosts in the spread of influenza from birds to humans but studies of the sialylated glycans that comprise their respiratory tract have not been extensively studied in the past. This study analyzed the sialylated N-glycan and O-glycan profile of swine trachea and lung and correlated this with ex-vivo infection of swine explants with avian influenza viruses. SAMPLE: Lungs and tracheal samples were obtained from normal farm and laboratory raised swine and used for ex vivo infection as well as mass spectrometric analysis. Infection of the ex vivo tissues used high pathogenic and low pathogenic avian viruses including the novel H7N9 virus that emerged in China in early 2013. MAIN OUTCOME MEASURES: Assessment of successful replication was determined by TCID50 as well as virus immunohistochemistry. The N-glycan and O-glycan profiles were measured by MALDI-TOF and sialylated linkages were determined by sialidase treatment. Lectin binding histochemistry was also performed on formalin fixed tissue samples with positive binding detected by chromogen staining. RESULTS: The swine respiratory tract glycans differed from the human respiratory tact glycans in two main areas. There was a greater abundance of Gal-α-Gal linkages resulting in a relative decrease in sialylated glycans. The swine respiratory tract also had a greater proportion of glycans containing Neu5Gc and Siaα2-6 glycans than the human respiratory tract. Infection with avian viruses was confined primarily to lung bronchioles rather than trachea and parenchyma. CONCLUSIONS: In contrast to previous studies we found that there was not as much expression of Siaα2-3 glycans on the surface of the trachea. Infection of Siaα2-3 binding avian viruses was restricted to the lower respiratory tract bronchioles. This finding may diminish the ability of the swine to act as an intermediary in the transmission of avian viruses to humans.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/subs.asp?ref=1750-2640&site=1en_US
dc.relation.ispartofInfluenza and Other Respiratory Virusesen_US
dc.rightsThe definitive version is available at www.blackwell-synergy.comen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleInfection of swine ex vivo tissues with avian viruses including H7N9 and correlation with glycomic analysisen_US
dc.typeArticleen_US
dc.identifier.emailChan, WY: reneewy@hku.hken_US
dc.identifier.emailChan, WW: wwic@hku.hken_US
dc.identifier.emailChan, MCW: mchan@hku.hken_US
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_US
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_US
dc.identifier.emailNicholls, JM: jmnichol@hkucc.hku.hken_US
dc.identifier.authorityChan, WY=rp01596en_US
dc.identifier.authorityChan, MCW=rp00420en_US
dc.identifier.authorityPeiris, JSM=rp00410en_US
dc.identifier.authorityGuan, Y=rp00397en_US
dc.identifier.authorityNicholls, JM=rp00364en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1111/irv.12144en_US
dc.identifier.pmid24001121-
dc.identifier.hkuros227936en_US
dc.identifier.hkuros217210-
dc.identifier.volume7en_US
dc.identifier.issue6-
dc.identifier.spage1269en_US
dc.identifier.epage1282en_US
dc.identifier.isiWOS:000331001400054-
dc.publisher.placeUnited Kingdom-

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