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Conference Paper: The role of tumor suppressing microRNA let-7 in undifferentiated nasopharyngeal carcinoma

TitleThe role of tumor suppressing microRNA let-7 in undifferentiated nasopharyngeal carcinoma
Authors
KeywordsMedical sciences
Oncology
Issue Date2012
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
The 103rd Annual Meeting of the America Association for Cancer Research (AACR 2012), Chicago, IL., 31 March-4 April 2012. In Cancer Research, 2012, v. 72 n. 8 suppl. 1, abstract no. LB-385 How to Cite?
AbstractAIMS:This study aimed at evaluating the potential anti-proliferative effects of the microRNA let-7 family in nasopharyngeal carcinoma (NPC) cells. In addition, the association between let-7 suppression and DNA hypermethylation is examined. MATERIALS AND METHODS:Levels of mature let-7 family members (-a, -b, -d, -e, -g, and -i) in normal nasopharyngeal cells (NP69 and NP460) and nasopharyngeal carcinoma cells (HK1 and HONE1) were measured by real-time quantitative PCR. Cell-proliferation assay and c-Myc immunohistochemical staining were performed on NPC cells transfected with let-7 precursor molecules. In addition, expression changes in let-7 family members in response to demethylating agents (5-azacytidine and zebularine) were also examined. RESULTS:In comparison with the normal nasopharyngeal cells, let-7 (-a, -b, -d, -e, -g, and -i) levels were reduced in nasopharyngeal carcinoma cells. Ectopic expression of the let-7 family in nasopharyngeal carcinoma cells resulted in inhibition of cell proliferation through downregulation of c-Myc expression. Demethylation treatment of nasopharyngeal carcinoma cells caused activation of let-7 expression in poorly differentiated nasopharyngeal carcinoma cells only. CONCLUSION:Our results suggested that miRNA let-7 might play a role in the proliferation of NPC. DNA methylation is a potential regulatory pathway, which is affected when let-7 is suppressed in NPC cells. However, the extent of DNA hypermethylation/hypomethylation in regulating let-7 expression requires further elucidation.
DescriptionConference Theme: Accelerating Science: Concept to Clinic
Poster Session 37: Late-Breaking Poster Presentations - Epigenetics: abstract no. LB-385
This journal suppl. entitled: Proceedings: AACR 103rd Annual Meeting ... 2012
Persistent Identifierhttp://hdl.handle.net/10722/194797
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorWong, TSen_US
dc.contributor.authorGao, Wen_US
dc.contributor.authorLi, JZHen_US
dc.contributor.authorChan, JYW-
dc.date.accessioned2014-02-17T02:10:25Z-
dc.date.available2014-02-17T02:10:25Z-
dc.date.issued2012en_US
dc.identifier.citationThe 103rd Annual Meeting of the America Association for Cancer Research (AACR 2012), Chicago, IL., 31 March-4 April 2012. In Cancer Research, 2012, v. 72 n. 8 suppl. 1, abstract no. LB-385en_US
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/194797-
dc.descriptionConference Theme: Accelerating Science: Concept to Clinic-
dc.descriptionPoster Session 37: Late-Breaking Poster Presentations - Epigenetics: abstract no. LB-385-
dc.descriptionThis journal suppl. entitled: Proceedings: AACR 103rd Annual Meeting ... 2012-
dc.description.abstractAIMS:This study aimed at evaluating the potential anti-proliferative effects of the microRNA let-7 family in nasopharyngeal carcinoma (NPC) cells. In addition, the association between let-7 suppression and DNA hypermethylation is examined. MATERIALS AND METHODS:Levels of mature let-7 family members (-a, -b, -d, -e, -g, and -i) in normal nasopharyngeal cells (NP69 and NP460) and nasopharyngeal carcinoma cells (HK1 and HONE1) were measured by real-time quantitative PCR. Cell-proliferation assay and c-Myc immunohistochemical staining were performed on NPC cells transfected with let-7 precursor molecules. In addition, expression changes in let-7 family members in response to demethylating agents (5-azacytidine and zebularine) were also examined. RESULTS:In comparison with the normal nasopharyngeal cells, let-7 (-a, -b, -d, -e, -g, and -i) levels were reduced in nasopharyngeal carcinoma cells. Ectopic expression of the let-7 family in nasopharyngeal carcinoma cells resulted in inhibition of cell proliferation through downregulation of c-Myc expression. Demethylation treatment of nasopharyngeal carcinoma cells caused activation of let-7 expression in poorly differentiated nasopharyngeal carcinoma cells only. CONCLUSION:Our results suggested that miRNA let-7 might play a role in the proliferation of NPC. DNA methylation is a potential regulatory pathway, which is affected when let-7 is suppressed in NPC cells. However, the extent of DNA hypermethylation/hypomethylation in regulating let-7 expression requires further elucidation.-
dc.languageengen_US
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/-
dc.relation.ispartofCancer Researchen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectMedical sciences-
dc.subjectOncology-
dc.titleThe role of tumor suppressing microRNA let-7 in undifferentiated nasopharyngeal carcinomaen_US
dc.typeConference_Paperen_US
dc.identifier.emailWong, TS: wongtsa@hkucc.hku.hken_US
dc.identifier.emailGao, W: weigaoi@hku.hken_US
dc.identifier.emailChan, JYW: jywchan1@hku.hken_US
dc.identifier.authorityWong, TS=rp00478en_US
dc.identifier.authorityChan, JYW=rp01314en_US
dc.description.naturepostprint-
dc.identifier.doi10.1158/1538-7445.AM2012-LB-385-
dc.identifier.hkuros227804en_US
dc.identifier.volume72-
dc.identifier.issue8 suppl. 1-
dc.publisher.placeUnited States-

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