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Article: The topoisomerase II-associated protein, Pat1p, is required for maintenance of rDNA locus stability in Saccharomyces cerevisiae

TitleThe topoisomerase II-associated protein, Pat1p, is required for maintenance of rDNA locus stability in Saccharomyces cerevisiae
Authors
Issue Date1999
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htm
Citation
Molecular and General Genetics, 1999, v. 261 n. 4-5, p. 831-840 How to Cite?
AbstractThe Pat1 protein of Saccharomyces cerevisiae was identified during a screen for proteins that interact with topoisomerase II. Previously, we have shown that pat1 delta mutants exhibit a slow-growth phenotype and an elevated frequency of both mitotic and meiotic chromosome mis-segregation. Here, we have studied the effects of deleting the PAT1 gene on chromosomal stability, with particular reference to rates of homologous recombination within the rDNA locus. This locus was analyzed because rDNA-specific hyperrecombination is known to occur in conditional top2 mutants. We show that pat1 delta strains mimic top2 mutants in displaying an elevated rate of intrachromosomal excision recombination at the rDNA locus, but not elsewhere in the genome. The elevated rate of recombination is dependent upon Rad52p, but not upon Rad51p or Rad54p. However, pat1 delta strains display additional manifestations of more general genomic instability, in that they show mild sensitivity to UV light and an increased incidence of interchromosomal recombination between heteroalleles.
Persistent Identifierhttp://hdl.handle.net/10722/194592
ISSN
2003 Impact Factor: 2.727

 

DC FieldValueLanguage
dc.contributor.authorWang, X-
dc.contributor.authorWatt, PM-
dc.contributor.authorBorts, RH-
dc.contributor.authorLouis, EJ-
dc.contributor.authorHickson, ID-
dc.date.accessioned2014-02-13T04:48:08Z-
dc.date.available2014-02-13T04:48:08Z-
dc.date.issued1999-
dc.identifier.citationMolecular and General Genetics, 1999, v. 261 n. 4-5, p. 831-840-
dc.identifier.issn0026-8925-
dc.identifier.urihttp://hdl.handle.net/10722/194592-
dc.description.abstractThe Pat1 protein of Saccharomyces cerevisiae was identified during a screen for proteins that interact with topoisomerase II. Previously, we have shown that pat1 delta mutants exhibit a slow-growth phenotype and an elevated frequency of both mitotic and meiotic chromosome mis-segregation. Here, we have studied the effects of deleting the PAT1 gene on chromosomal stability, with particular reference to rates of homologous recombination within the rDNA locus. This locus was analyzed because rDNA-specific hyperrecombination is known to occur in conditional top2 mutants. We show that pat1 delta strains mimic top2 mutants in displaying an elevated rate of intrachromosomal excision recombination at the rDNA locus, but not elsewhere in the genome. The elevated rate of recombination is dependent upon Rad52p, but not upon Rad51p or Rad54p. However, pat1 delta strains display additional manifestations of more general genomic instability, in that they show mild sensitivity to UV light and an increased incidence of interchromosomal recombination between heteroalleles.-
dc.languageeng-
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00438/index.htm-
dc.relation.ispartofMolecular and General Genetics-
dc.rightsThe original publication is available at www.springerlink.com-
dc.subject.meshDNA Topoisomerases, Type II - genetics - metabolism-
dc.subject.meshDNA, Ribosomal - genetics-
dc.subject.meshDNA-Binding Proteins - metabolism-
dc.subject.meshSaccharomyces cerevisiae - genetics - metabolism-
dc.subject.meshSaccharomyces cerevisiae Proteins-
dc.titleThe topoisomerase II-associated protein, Pat1p, is required for maintenance of rDNA locus stability in Saccharomyces cerevisiaeen_US
dc.typeArticleen_US
dc.identifier.emailWang, X: xqwang@hkucc.hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s004380050027-
dc.identifier.pmid10394921-
dc.identifier.scopuseid_2-s2.0-0033035375-
dc.identifier.volume261-
dc.identifier.issue4-5-
dc.identifier.spage831-
dc.identifier.epage840-
dc.publisher.placeGermany-

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