IMMUNOGLOBULIN HAVING PARTICULAR FRAMEWORK SCAFFOLD AND METHODS OF MAKING AND USING

Abstract

This invention relates to immunoglobulin molecules comprising light chain (VL) chimeric variable domains, heavy chain (VH) chimeric variable domains, e.g., scFv antibodies that are expressed at high levels within a host cell, preferably within particular cellular compartments such as, e.g., cytosol or apoplast. The VL, VH and scFv antibody molecules comprise framework scaffolds of particularly preferred framework regions. This invention also relates to nucleic acid molecules encoding the immunoglobulin molecules of this invention, vectors expressing the immunoglobulin molecules, hosts transformed with the nucleic acid molecules and vectors, and methods of using the immunoglobulin molecules. Also described are immunoglobulin libraries as well as host cells, including transgenic plants, expressing the VL, VH or scFv antibody molecules of this invention.