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Article: Perfluorooctanoate suppresses spheroid attachment on endometrial epithelial cells through peroxisome proliferator-activated receptor alpha and down-regulation of Wnt signaling

TitlePerfluorooctanoate suppresses spheroid attachment on endometrial epithelial cells through peroxisome proliferator-activated receptor alpha and down-regulation of Wnt signaling
Authors
Issue Date2013
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/reprotox
Citation
Reproductive Toxicology, 2013, v. 42, p. 164-171 How to Cite?
AbstractExposure of animals to perfluorooctanoic acid (PFOA), a surfactant used in emulsion polymerization processes causes early pregnancy loss, delayed growth and development of fetuses. The mechanisms of action are largely unknown. We studied the effect of PFOA on implantation using an in vitro spheroid-endometrial cell co-culture model. PFOA (10-100μM) significantly reduced Jeg-3 spheroid attachment on RL95-2 endometrial cells. PFOA also suppressed β-catenin expression in Jeg-3 cells. The Wnt agonist Wnt3a stimulated β-catenin expression in Jeg-3 cells and reversed the PFOA suppression of the spheroid attachment. The putative PFOA receptors (PPARα, β, γ) present in both cell lines were not affected by PFOA (0.01-100μM). The PPARα antagonist MK886 restored the β-catenin and E-cadherin expression levels in Jeg-3 cells and reversed the suppression of the spheroid attachment caused by PFOA. Taken together, PFOA suppresses spheroid attachment through PPARα and Wnt signaling pathways via down-regulation of β-catenin and E-cadherin expression.
Persistent Identifierhttp://hdl.handle.net/10722/193619
ISSN
2015 Impact Factor: 2.85
2015 SCImago Journal Rankings: 1.263
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsang, Hen_US
dc.contributor.authorCheung, TYen_US
dc.contributor.authorKodithuwakku, SPKen_US
dc.contributor.authorChai, Jen_US
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorWong, CKen_US
dc.contributor.authorLee, CKFen_US
dc.date.accessioned2014-01-20T05:10:01Z-
dc.date.available2014-01-20T05:10:01Z-
dc.date.issued2013en_US
dc.identifier.citationReproductive Toxicology, 2013, v. 42, p. 164-171en_US
dc.identifier.issn0890-6238-
dc.identifier.urihttp://hdl.handle.net/10722/193619-
dc.description.abstractExposure of animals to perfluorooctanoic acid (PFOA), a surfactant used in emulsion polymerization processes causes early pregnancy loss, delayed growth and development of fetuses. The mechanisms of action are largely unknown. We studied the effect of PFOA on implantation using an in vitro spheroid-endometrial cell co-culture model. PFOA (10-100μM) significantly reduced Jeg-3 spheroid attachment on RL95-2 endometrial cells. PFOA also suppressed β-catenin expression in Jeg-3 cells. The Wnt agonist Wnt3a stimulated β-catenin expression in Jeg-3 cells and reversed the PFOA suppression of the spheroid attachment. The putative PFOA receptors (PPARα, β, γ) present in both cell lines were not affected by PFOA (0.01-100μM). The PPARα antagonist MK886 restored the β-catenin and E-cadherin expression levels in Jeg-3 cells and reversed the suppression of the spheroid attachment caused by PFOA. Taken together, PFOA suppresses spheroid attachment through PPARα and Wnt signaling pathways via down-regulation of β-catenin and E-cadherin expression.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/reprotox-
dc.relation.ispartofReproductive Toxicologyen_US
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Reproductive Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Reproductive Toxicology, 2013, v. 42, p. 164-171. DOI: 10.1016/j.reprotox.2013.08.001-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titlePerfluorooctanoate suppresses spheroid attachment on endometrial epithelial cells through peroxisome proliferator-activated receptor alpha and down-regulation of Wnt signalingen_US
dc.typeArticleen_US
dc.identifier.emailChai, J: jchai@hkucc.hku.hken_US
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_US
dc.identifier.emailLee, CKF: ckflee@hku.hken_US
dc.identifier.authorityChai, J=rp00241en_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.identifier.authorityLee, CKF=rp00458en_US
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.reprotox.2013.08.001en_US
dc.identifier.pmid23978332-
dc.identifier.scopuseid_2-s2.0-84884513202-
dc.identifier.hkuros227375en_US
dc.identifier.volume42en_US
dc.identifier.spage164en_US
dc.identifier.epage171en_US
dc.identifier.isiWOS:000327569100018-
dc.publisher.placeUnited States-

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