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Conference Paper: GABAergic transmission in the critical period impacts on the thalamic spatial map and navigation behavior

TitleGABAergic transmission in the critical period impacts on the thalamic spatial map and navigation behavior
Authors
KeywordsVestibular
Thalamus
Gabaergic
Issue Date2013
PublisherSociety for Neuroscience.
Citation
The 43rd Annual Meeting of the Society for Neuroscience (SfN), San Diego, California, USA, 9-13 November 2013 How to Cite?
AbstractTo orient and navigate in the environment, the brain transforms vestibular cues into spatial orientation signals. Determining the role of neurotransmission within the neonatal vestibular nucleus (VN) in the establishment of a spatial map and in path integration is essential to our understanding of how spatial coding is shaped during development. Using whole-cell patch-clamp recording in brainstem slices, we found that most VN neurons of postnatal day (P) 3 - 5 rats exhibited long-term depression of GABAA receptor-mediated evoked-postsynaptic current. At this stage, these currents were excitatory in nature. By P14, long-term depression of these currents, which became inhibitory, was observed in only a small proportion of VN neurons. These results suggest a postnatal period during which plastic feature of GABAergic VN synapses is observed. To further study the role of GABAergic transmission in VN on developing acquisition of spatial recognition, we implanted above the VN of P1 or P14 rats with Elvax slice loaded with GABAA receptor antagonist bicuculline. These pups were allowed to recover and studied at different ages. Derangement of a vestibular-related spatial map in thalamus, an upstream relay of the neural circuitry for balance, was observed in adult rats pre-treated with bicuculline at P1 but not in those pre-treated at P14. When tested with a path integration task at the adult stage, rats pretreated with bicuculline at P1 had significantly prolonged training time and deficiency in behavioral parameters (including searching time, returning time, heading angle, and number of errors in dark/ new location probe tests). Comparable deficits were also observed with selective lesion of vestibular-related subnuclei in the adult thalamus. Rats pretreated at P14 however showed no significant difference in light, dark, and new location probe tests. Taken together, we have demonstrated that a postnatal critical period exists for developmental establishment of a neural circuitry for spatial reference and acquisition of spatial navigation. [Supported by RGC 761711M]
DescriptionPoster session 164: Vestibular System: Central Physiology and Anatomy
Program no. 164.10 & Poster no. MM2
Fulltext of the abstract in: http://www.abstractsonline.com/plan/ViewAbstract.aspx?cKey=7de7566f-7638-4849-bcde-911681b7e7e8&mID=3236&mKey=8d2a5bec-4825-4cd6-9439-b42bb151d1cf&sKey=af7939d7-709f-4f17-afd0-c66a67904316
Persistent Identifierhttp://hdl.handle.net/10722/193281

 

DC FieldValueLanguage
dc.contributor.authorChan, YSen_US
dc.contributor.authorChen, Wen_US
dc.contributor.authorMa, CWen_US
dc.contributor.authorShi, Wen_US
dc.contributor.authorNogueira Botelho, FPen_US
dc.contributor.authorLai, CHen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2013-12-20T02:43:49Z-
dc.date.available2013-12-20T02:43:49Z-
dc.date.issued2013en_US
dc.identifier.citationThe 43rd Annual Meeting of the Society for Neuroscience (SfN), San Diego, California, USA, 9-13 November 2013en_US
dc.identifier.urihttp://hdl.handle.net/10722/193281-
dc.descriptionPoster session 164: Vestibular System: Central Physiology and Anatomy-
dc.descriptionProgram no. 164.10 & Poster no. MM2-
dc.descriptionFulltext of the abstract in: http://www.abstractsonline.com/plan/ViewAbstract.aspx?cKey=7de7566f-7638-4849-bcde-911681b7e7e8&mID=3236&mKey=8d2a5bec-4825-4cd6-9439-b42bb151d1cf&sKey=af7939d7-709f-4f17-afd0-c66a67904316-
dc.description.abstractTo orient and navigate in the environment, the brain transforms vestibular cues into spatial orientation signals. Determining the role of neurotransmission within the neonatal vestibular nucleus (VN) in the establishment of a spatial map and in path integration is essential to our understanding of how spatial coding is shaped during development. Using whole-cell patch-clamp recording in brainstem slices, we found that most VN neurons of postnatal day (P) 3 - 5 rats exhibited long-term depression of GABAA receptor-mediated evoked-postsynaptic current. At this stage, these currents were excitatory in nature. By P14, long-term depression of these currents, which became inhibitory, was observed in only a small proportion of VN neurons. These results suggest a postnatal period during which plastic feature of GABAergic VN synapses is observed. To further study the role of GABAergic transmission in VN on developing acquisition of spatial recognition, we implanted above the VN of P1 or P14 rats with Elvax slice loaded with GABAA receptor antagonist bicuculline. These pups were allowed to recover and studied at different ages. Derangement of a vestibular-related spatial map in thalamus, an upstream relay of the neural circuitry for balance, was observed in adult rats pre-treated with bicuculline at P1 but not in those pre-treated at P14. When tested with a path integration task at the adult stage, rats pretreated with bicuculline at P1 had significantly prolonged training time and deficiency in behavioral parameters (including searching time, returning time, heading angle, and number of errors in dark/ new location probe tests). Comparable deficits were also observed with selective lesion of vestibular-related subnuclei in the adult thalamus. Rats pretreated at P14 however showed no significant difference in light, dark, and new location probe tests. Taken together, we have demonstrated that a postnatal critical period exists for developmental establishment of a neural circuitry for spatial reference and acquisition of spatial navigation. [Supported by RGC 761711M]-
dc.languageengen_US
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofNeuroscience 2013en_US
dc.rightsNeuroscience 2013. Copyright © Society for Neuroscience.-
dc.subjectVestibular-
dc.subjectThalamus-
dc.subjectGabaergic-
dc.titleGABAergic transmission in the critical period impacts on the thalamic spatial map and navigation behavioren_US
dc.typeConference_Paperen_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailMa, CW: cwma2010@hku.hken_US
dc.identifier.emailLai, CH: chlaib@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.authorityLai, CH=rp00396en_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.identifier.hkuros226893en_US
dc.identifier.hkuros238205-
dc.publisher.placeUnited States-

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