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Article: Effects of 5F on IκKβ, IκB, p65, and p50 mRNA expression of non-small lung cancer NCI-H460 cells

TitleEffects of 5F on IκKβ, IκB, p65, and p50 mRNA expression of non-small lung cancer NCI-H460 cells
Authors
Issue Date2010
Citation
Chinese Traditional and Herbal Drugs, 2010, v. 41 n. 3, p. 435-439 How to Cite?
AbstractObjective: To investigate the inhibitory mechanism of 5F isolated from Pteris semipinnata on the proliferation and apoptosis induction of NCI-H460 cells. The roles of NF-κB pathway in the apoptosis were explored in this study. Methods: NCI-H460 Cell growth inhibition mediated by 5F was detected by MTT assay. RT-PCR was Applied to examing the mRNA levels of IκKβ, IκB, p65, and p50. Results: 5F Showed the growth inhibitory activity against NCI-H460 cells with IC50 values of 21.40, 4.52, and 1.02 μg/mL for 24, 48, and 72 h, respectively. The mRNA expression levels of IκKβ and IκB were significantly decreased after 5F treated cells for 6 h (P<0.05); The mRNA expression levels of p65 and p50 were significantly decreased after 5F treated cells for 3 h (P<0.05). Conclusion: The mechanism for 5F induction of NCI-H460 cells apoptosis could be mediated by the inhibition of NF-κB pathway.
Persistent Identifierhttp://hdl.handle.net/10722/192677
ISSN
2015 SCImago Journal Rankings: 0.130

 

DC FieldValueLanguage
dc.contributor.authorLiu, Yen_US
dc.contributor.authorChen, GGen_US
dc.contributor.authorLü, Y-Nen_US
dc.contributor.authorHsin, MKYen_US
dc.contributor.authorUnderwood, MJen_US
dc.contributor.authorLiang, N-Cen_US
dc.date.accessioned2013-11-20T04:55:10Z-
dc.date.available2013-11-20T04:55:10Z-
dc.date.issued2010en_US
dc.identifier.citationChinese Traditional and Herbal Drugs, 2010, v. 41 n. 3, p. 435-439en_US
dc.identifier.issn0253-2670en_US
dc.identifier.urihttp://hdl.handle.net/10722/192677-
dc.description.abstractObjective: To investigate the inhibitory mechanism of 5F isolated from Pteris semipinnata on the proliferation and apoptosis induction of NCI-H460 cells. The roles of NF-κB pathway in the apoptosis were explored in this study. Methods: NCI-H460 Cell growth inhibition mediated by 5F was detected by MTT assay. RT-PCR was Applied to examing the mRNA levels of IκKβ, IκB, p65, and p50. Results: 5F Showed the growth inhibitory activity against NCI-H460 cells with IC50 values of 21.40, 4.52, and 1.02 μg/mL for 24, 48, and 72 h, respectively. The mRNA expression levels of IκKβ and IκB were significantly decreased after 5F treated cells for 6 h (P<0.05); The mRNA expression levels of p65 and p50 were significantly decreased after 5F treated cells for 3 h (P<0.05). Conclusion: The mechanism for 5F induction of NCI-H460 cells apoptosis could be mediated by the inhibition of NF-κB pathway.en_US
dc.languageengen_US
dc.relation.ispartofChinese Traditional and Herbal Drugsen_US
dc.titleEffects of 5F on IκKβ, IκB, p65, and p50 mRNA expression of non-small lung cancer NCI-H460 cellsen_US
dc.typeArticleen_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-77952717201en_US
dc.identifier.volume41en_US
dc.identifier.issue3en_US
dc.identifier.spage435en_US
dc.identifier.epage439en_US

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