File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: The inhibitory effects of silver diamine fluorides on cysteine cathepsins

TitleThe inhibitory effects of silver diamine fluorides on cysteine cathepsins
Authors
KeywordsCariology
Dentin
Fluoride and cysteine cathepsins
Enzymes
Issue Date2013
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 78 How to Cite?
AbstractObjective: The expression of cysteine cathepsins in human carious dentin suggests that this enzyme contributes to the collagen degradation in caries progress. This study investigated whether silver diamine fluoride (SDF) inhibited the activity of cysteine cathepsins. Method: Three commercial SDF solutions with concentrations at 38%, 30% and 12% were studied. This study prepared two sodium fluoride solutions with the same fluoride ion (F-) concentrations as the 38% and 12% SDF solutions, and 2 silver nitrate solutions with the same silver ion (Ag+) concentrations as the 38% and 12% SDF solutions. Five samples of each experimental solution were used to study their inhibitory effect on two cathepsins, cathepsin B and cathepsin K, using cathepsin assay kits. Control solution containing assay buffer and cathepsin dilution was used to calculate the percentage inhibition (difference between the mean readings of the test and the control groups divided by that of the control group). Result: The percentage inhibition of 38%, 30% and 12% SDF on cathepsin B were 92.0%, 91.5% and 90.3%, respectively (p<0.001); on cathepsin K were 80.6%, 78.5% and 77.9%, respectively (p<0.001). Two-way ANOVA demonstrated that Ag+ and F- had interaction inhibitory effect in both cathepsin B and K. Besides, Ag+ was significantly more effective in inhibition of cathepsins B and K activity than F-(p<0.01). Conclusion: Higher concentrations of SDF solution have greater inhibitory effect on cathepsins B and K. Furthermore, Ag+ is more effective than F- in inhibiting the activity of cathepsins B and K. This abstract is based on research that was funded entirely or partially by an outside source: This study was supported by the NSFC/RGC Joint Research Scheme (Grant No. N_HKU 776/10 and NSFC No.81061160511).
DescriptionConference Theme: We are the Future
Poster Presentation
Session 10: P1
Persistent Identifierhttp://hdl.handle.net/10722/192588
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorChu, CHen_US
dc.contributor.authorMei, Len_US
dc.contributor.authorLo, ECMen_US
dc.contributor.authorCao, Yen_US
dc.contributor.authorLi, Qen_US
dc.date.accessioned2013-11-18T05:06:51Z-
dc.date.available2013-11-18T05:06:51Z-
dc.date.issued2013en_US
dc.identifier.citationThe 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 78en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/192588-
dc.descriptionConference Theme: We are the Future-
dc.descriptionPoster Presentation-
dc.descriptionSession 10: P1-
dc.description.abstractObjective: The expression of cysteine cathepsins in human carious dentin suggests that this enzyme contributes to the collagen degradation in caries progress. This study investigated whether silver diamine fluoride (SDF) inhibited the activity of cysteine cathepsins. Method: Three commercial SDF solutions with concentrations at 38%, 30% and 12% were studied. This study prepared two sodium fluoride solutions with the same fluoride ion (F-) concentrations as the 38% and 12% SDF solutions, and 2 silver nitrate solutions with the same silver ion (Ag+) concentrations as the 38% and 12% SDF solutions. Five samples of each experimental solution were used to study their inhibitory effect on two cathepsins, cathepsin B and cathepsin K, using cathepsin assay kits. Control solution containing assay buffer and cathepsin dilution was used to calculate the percentage inhibition (difference between the mean readings of the test and the control groups divided by that of the control group). Result: The percentage inhibition of 38%, 30% and 12% SDF on cathepsin B were 92.0%, 91.5% and 90.3%, respectively (p<0.001); on cathepsin K were 80.6%, 78.5% and 77.9%, respectively (p<0.001). Two-way ANOVA demonstrated that Ag+ and F- had interaction inhibitory effect in both cathepsin B and K. Besides, Ag+ was significantly more effective in inhibition of cathepsins B and K activity than F-(p<0.01). Conclusion: Higher concentrations of SDF solution have greater inhibitory effect on cathepsins B and K. Furthermore, Ag+ is more effective than F- in inhibiting the activity of cathepsins B and K. This abstract is based on research that was funded entirely or partially by an outside source: This study was supported by the NSFC/RGC Joint Research Scheme (Grant No. N_HKU 776/10 and NSFC No.81061160511).-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectCariology-
dc.subjectDentin-
dc.subjectFluoride and cysteine cathepsins-
dc.subjectEnzymes-
dc.titleThe inhibitory effects of silver diamine fluorides on cysteine cathepsinsen_US
dc.typeConference_Paperen_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.emailMei, L: mei1123@hku.hken_US
dc.identifier.emailLo, ECM: hrdplcm@hkucc.hku.hken_US
dc.identifier.authorityChu, CH=rp00022en_US
dc.identifier.authorityMei, L=rp01840en_US
dc.identifier.authorityLo, ECM=rp00015en_US
dc.identifier.hkuros226818en_US
dc.identifier.hkuros226756-
dc.identifier.hkuros226764-
dc.identifier.volume92en_US
dc.identifier.issueSpecial Issue B: abstract no. 78en_US
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats