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Conference Paper: Remineralization of Dentin in hydrogel microenvirment aided by Electrophoresis

TitleRemineralization of Dentin in hydrogel microenvirment aided by Electrophoresis
Authors
KeywordsBiomimetic mineralization
Dental materials and Dentin
Issue Date2013
PublisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 736 How to Cite?
AbstractObjective: Remineralization of dentin collagen matrix is a challenge work, and the velocity of remineralization is too slow for its clinic application. Herein, we developed a system to quickly remineralize dentin collagen matrix with calcium and phosphate loaded hydrogel microenvirment aiding with electrophoresis. Method: The hydrogel mineralization system was constructed by 1% agarose gel containing 0.13 M CaCl2 ,1% pure gel, and 1% agarose gel containing 0.26 M Na2HPO4 in proper sequence, and the acid-etched dentin (37% H3PO4 for 2 min)was standing vertically in the junction of pure gel and Na2HPO4 loaded gel, which was connected to a electrophoresis apparatus (U=50V, I=20mA).electrophoresis for 2 hours was used as a mineralization cycle, and the all the gel systems were refreshed for the following mineralization cycles. Result: After 6 cycles of mineralization, FTIR and XRD confirmed the mineral precipitates in the dentin slices were hydroxyapatite (HAp). SEM results showed that demineralized dentin collagen matrix were remineralized with intrafibrillar and interfibrillar HAp formation to duplicate the calcified dentin collagen matrix, the precipitates occluded the dentinal tubules, and the needle HA crystals densely packing with each other similar to enamel precipitated on remineralized dentin surface. Nanoindentation and microhardness results showed that the remineralization improved mechanical property of demineralized dentin. Conclusion: A novel system of quick mineralization of dentin, which contained calcium and phosphate loaded agarose hydrogel microenvirment aiding with electrophoresis, was developed. The system may be a good candidate to treat dentin hypersensitivity, and other dentin defects.
DescriptionConference Theme: We are the Future
Oral Presentation
Session 29: O20
Persistent Identifierhttp://hdl.handle.net/10722/192516
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorWu, Xen_US
dc.contributor.authorXu, Qen_US
dc.contributor.authorLi, Qen_US
dc.contributor.authorCao, Yen_US
dc.contributor.authorChu, CHen_US
dc.date.accessioned2013-11-15T02:20:46Z-
dc.date.available2013-11-15T02:20:46Z-
dc.date.issued2013en_US
dc.identifier.citationThe 2nd Meeting of the International Association of Dental Research - Asia Pacific Region (IADR-APR), Bangkok, Thailand, 21-23 August 2013. In Journal of Dental Research, 2013, v. 92 n. Special Issue B: abstract no. 736en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/192516-
dc.descriptionConference Theme: We are the Future-
dc.descriptionOral Presentation-
dc.descriptionSession 29: O20-
dc.description.abstractObjective: Remineralization of dentin collagen matrix is a challenge work, and the velocity of remineralization is too slow for its clinic application. Herein, we developed a system to quickly remineralize dentin collagen matrix with calcium and phosphate loaded hydrogel microenvirment aiding with electrophoresis. Method: The hydrogel mineralization system was constructed by 1% agarose gel containing 0.13 M CaCl2 ,1% pure gel, and 1% agarose gel containing 0.26 M Na2HPO4 in proper sequence, and the acid-etched dentin (37% H3PO4 for 2 min)was standing vertically in the junction of pure gel and Na2HPO4 loaded gel, which was connected to a electrophoresis apparatus (U=50V, I=20mA).electrophoresis for 2 hours was used as a mineralization cycle, and the all the gel systems were refreshed for the following mineralization cycles. Result: After 6 cycles of mineralization, FTIR and XRD confirmed the mineral precipitates in the dentin slices were hydroxyapatite (HAp). SEM results showed that demineralized dentin collagen matrix were remineralized with intrafibrillar and interfibrillar HAp formation to duplicate the calcified dentin collagen matrix, the precipitates occluded the dentinal tubules, and the needle HA crystals densely packing with each other similar to enamel precipitated on remineralized dentin surface. Nanoindentation and microhardness results showed that the remineralization improved mechanical property of demineralized dentin. Conclusion: A novel system of quick mineralization of dentin, which contained calcium and phosphate loaded agarose hydrogel microenvirment aiding with electrophoresis, was developed. The system may be a good candidate to treat dentin hypersensitivity, and other dentin defects.-
dc.languageengen_US
dc.publisherSage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc.-
dc.subjectBiomimetic mineralization-
dc.subjectDental materials and Dentin-
dc.titleRemineralization of Dentin in hydrogel microenvirment aided by Electrophoresisen_US
dc.typeConference_Paperen_US
dc.identifier.emailLi, Q: ql-li@126.comen_US
dc.identifier.emailChu, CH: chchu@hku.hken_US
dc.identifier.authorityChu, CH=rp00022en_US
dc.identifier.hkuros226771en_US
dc.identifier.volume92en_US
dc.identifier.issueSpecial Issue B: abstract no. 736-
dc.publisher.placeUnited States-

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