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Conference Paper: A new role for marine skeletal proteins in regenerative orthopaedics

TitleA new role for marine skeletal proteins in regenerative orthopaedics
Authors
KeywordsAdult Mesenchymal Stem Cells
Marine Bioceramics
Osteoinduction
Proteomics
Issue Date2012
PublisherTrans Tech Publications. The Journal's web site is located at http://www.scientific.net
Citation
The 24th Symposium and Annual Meeting of the International Society for Ceramics in Medicine (ISCM 2012), Fukuoka, Japan, 21-24 October 2012. In Key Engineering Materials, 2012, v. 529, p. 654-659 How to Cite?
AbstractUse of ready-made marine skeletons is one of the simplest possible remedies to major problems hindering the future development of regenerative orthopaedics- such as, providing a richness of framework designs and now a potentially rich, accessible source of osteopromotive analogues and biomineralisation proteins. It has already been shown that coral and marine sponge skeletons can support self-sustaining musculoskeletal tissues and that extracts of spongin collagen and nacre seashell organic matrices promote bone mineralisation. This should not be surprising given that the pivotal biomineralisation proteins, which orchestrate bone morphogenesis are also found in the earliest calcifying marine organisms. This is because they are representatives of the first molecular components established for calcification, morphogenesis and wound healing. In support of this notion, it has emerged that BMP molecules- the main cluster of bone growth factors for human bone morphogenesis- are secreted by endodermal cells into the developing skeleton. In addition, the regenerative signalling proteins, TGF-b and Wnt-prime targets in bone therapeutics- are also present in early marine sponge development and instrumental to stem cell activation in Cnidarians. Based on this match between vertebrate and invertebrate main developmental proteins, we review the nature and extent of this evolutionary relatedness and use it to support the development of a new strategy, which is to mine selected marine origin organic matrices for novel metabolic, signalling and structural proteins/peptides and protein analogues to apply in regenerative orthopaedics, particularly when using adult stem cells. To support such a proposal we show early stage evidence-gathered in our own laboratory- of the presence of fibrinogen fragments and early osteopromotive effects of a coral organic matrix extract on stem cells. In practice the discovery of new osteopromotive and osteo-accelerant protein analogues will require use of traditional chromatography techniques, osteoactivity assays to hone in on potential proteins of significance and advanced proteomic tools to provide accurate sequencing, determine the mechanisms and molecular pathways involved in osteoactivation and determine the efficiency and effectiveness of marine skeleton-derived protein modulation of the stem cell (MSC) proteome. As more analogues are discovered using proteomic tools, skeletal organic matrices may have ever increasing utility for regenerative orthopaedics.
DescriptionChapter 13. Sol-Gel Synthesis and Other Materials
Persistent Identifierhttp://hdl.handle.net/10722/191568
ISBN

 

DC FieldValueLanguage
dc.contributor.authorGreen, DWen_US
dc.contributor.authorPadula, MPen_US
dc.contributor.authorSantos, Jen_US
dc.contributor.authorChou, Jen_US
dc.contributor.authorMilthorpe, Ben_US
dc.contributor.authorBen-Nissan, Ben_US
dc.date.accessioned2013-10-15T07:11:22Z-
dc.date.available2013-10-15T07:11:22Z-
dc.date.issued2012en_US
dc.identifier.citationThe 24th Symposium and Annual Meeting of the International Society for Ceramics in Medicine (ISCM 2012), Fukuoka, Japan, 21-24 October 2012. In Key Engineering Materials, 2012, v. 529, p. 654-659en_US
dc.identifier.isbn9783037855171-
dc.identifier.urihttp://hdl.handle.net/10722/191568-
dc.descriptionChapter 13. Sol-Gel Synthesis and Other Materials-
dc.description.abstractUse of ready-made marine skeletons is one of the simplest possible remedies to major problems hindering the future development of regenerative orthopaedics- such as, providing a richness of framework designs and now a potentially rich, accessible source of osteopromotive analogues and biomineralisation proteins. It has already been shown that coral and marine sponge skeletons can support self-sustaining musculoskeletal tissues and that extracts of spongin collagen and nacre seashell organic matrices promote bone mineralisation. This should not be surprising given that the pivotal biomineralisation proteins, which orchestrate bone morphogenesis are also found in the earliest calcifying marine organisms. This is because they are representatives of the first molecular components established for calcification, morphogenesis and wound healing. In support of this notion, it has emerged that BMP molecules- the main cluster of bone growth factors for human bone morphogenesis- are secreted by endodermal cells into the developing skeleton. In addition, the regenerative signalling proteins, TGF-b and Wnt-prime targets in bone therapeutics- are also present in early marine sponge development and instrumental to stem cell activation in Cnidarians. Based on this match between vertebrate and invertebrate main developmental proteins, we review the nature and extent of this evolutionary relatedness and use it to support the development of a new strategy, which is to mine selected marine origin organic matrices for novel metabolic, signalling and structural proteins/peptides and protein analogues to apply in regenerative orthopaedics, particularly when using adult stem cells. To support such a proposal we show early stage evidence-gathered in our own laboratory- of the presence of fibrinogen fragments and early osteopromotive effects of a coral organic matrix extract on stem cells. In practice the discovery of new osteopromotive and osteo-accelerant protein analogues will require use of traditional chromatography techniques, osteoactivity assays to hone in on potential proteins of significance and advanced proteomic tools to provide accurate sequencing, determine the mechanisms and molecular pathways involved in osteoactivation and determine the efficiency and effectiveness of marine skeleton-derived protein modulation of the stem cell (MSC) proteome. As more analogues are discovered using proteomic tools, skeletal organic matrices may have ever increasing utility for regenerative orthopaedics.-
dc.languageengen_US
dc.publisherTrans Tech Publications. The Journal's web site is located at http://www.scientific.net-
dc.relation.ispartofKey Engineering Materialsen_US
dc.subjectAdult Mesenchymal Stem Cells-
dc.subjectMarine Bioceramics-
dc.subjectOsteoinduction-
dc.subjectProteomics-
dc.titleA new role for marine skeletal proteins in regenerative orthopaedicsen_US
dc.typeConference_Paperen_US
dc.identifier.emailGreen, DW: dwgreen@hku.hken_US
dc.identifier.authorityGreen, DW=rp01598en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.4028/www.scientific.net/KEM.529-530-
dc.identifier.hkuros226472en_US
dc.identifier.volume529-
dc.identifier.spage654en_US
dc.identifier.epage659en_US

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