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Article: TLR5 signaling enhances the proliferation of human allogeneic CD40-activated B cell induced CD4hiCD25+ regulatory T cells
Title | TLR5 signaling enhances the proliferation of human allogeneic CD40-activated B cell induced CD4hiCD25+ regulatory T cells |
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Authors | |
Issue Date | 2013 |
Publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action |
Citation | Plos One, 2013, v. 8 n. 7, p. e67969 How to Cite? |
Abstract | Although diverse functions of different toll-like receptors (TLR) on human natural regulatory T cells have been demonstrated recently, the role of TLR-related signals on human induced regulatory T cells remain elusive. Previously our group developed an ex vivo high-efficient system in generating human alloantigen-specific CD4(hi)CD25(+) regulatory T cells from naive CD4(+)CD25(-) T cells using allogeneic CD40-activated B cells as stimulators. In this study, we investigated the role of TLR5-related signals on the generation and function of these novel CD4(hi)CD25(+) regulatory T cells. It was found that induced CD4(hi)CD25(+) regulatory T cells expressed an up-regulated level of TLR5 compared to their precursors. The blockade of TLR5 using anti-TLR5 antibodies during the co-culture decreased CD4(hi)CD25(+) regulatory T cells proliferation by induction of S phase arrest. The S phase arrest was associated with reduced ERK1/2 phosphorylation. However, TLR5 blockade did not decrease the CTLA-4, GITR and FOXP3 expressions, and the suppressive function of CD4(hi)CD25(+) regulatory T cells. In conclusion, we discovered a novel function of TLR5-related signaling in enhancing the proliferation of CD4(hi)CD25(+) regulatory T cells by promoting S phase progress but not involved in the suppressive function of human CD40-activated B cell-induced CD4(hi)CD25(+) regulatory T cells, suggesting a novel role of TLR5-related signals in the generation of induced regulatory T cells. |
Persistent Identifier | http://hdl.handle.net/10722/191460 |
ISSN | 2021 Impact Factor: 3.752 2020 SCImago Journal Rankings: 0.990 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chan, PL | en_US |
dc.contributor.author | Zheng, J | en_US |
dc.contributor.author | Liu, Y | en_US |
dc.contributor.author | Lam, KT | en_US |
dc.contributor.author | Xiang, Z | en_US |
dc.contributor.author | Mao, H | - |
dc.contributor.author | Liu, Y | - |
dc.contributor.author | Qin, G | - |
dc.contributor.author | Lau, YL | - |
dc.contributor.author | Tu, W | - |
dc.date.accessioned | 2013-10-15T07:01:21Z | - |
dc.date.available | 2013-10-15T07:01:21Z | - |
dc.date.issued | 2013 | en_US |
dc.identifier.citation | Plos One, 2013, v. 8 n. 7, p. e67969 | en_US |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10722/191460 | - |
dc.description.abstract | Although diverse functions of different toll-like receptors (TLR) on human natural regulatory T cells have been demonstrated recently, the role of TLR-related signals on human induced regulatory T cells remain elusive. Previously our group developed an ex vivo high-efficient system in generating human alloantigen-specific CD4(hi)CD25(+) regulatory T cells from naive CD4(+)CD25(-) T cells using allogeneic CD40-activated B cells as stimulators. In this study, we investigated the role of TLR5-related signals on the generation and function of these novel CD4(hi)CD25(+) regulatory T cells. It was found that induced CD4(hi)CD25(+) regulatory T cells expressed an up-regulated level of TLR5 compared to their precursors. The blockade of TLR5 using anti-TLR5 antibodies during the co-culture decreased CD4(hi)CD25(+) regulatory T cells proliferation by induction of S phase arrest. The S phase arrest was associated with reduced ERK1/2 phosphorylation. However, TLR5 blockade did not decrease the CTLA-4, GITR and FOXP3 expressions, and the suppressive function of CD4(hi)CD25(+) regulatory T cells. In conclusion, we discovered a novel function of TLR5-related signaling in enhancing the proliferation of CD4(hi)CD25(+) regulatory T cells by promoting S phase progress but not involved in the suppressive function of human CD40-activated B cell-induced CD4(hi)CD25(+) regulatory T cells, suggesting a novel role of TLR5-related signals in the generation of induced regulatory T cells. | - |
dc.language | eng | en_US |
dc.publisher | Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action | - |
dc.relation.ispartof | PLoS ONE | en_US |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject.mesh | Antigens, CD40 - immunology | - |
dc.subject.mesh | B-Lymphocytes - immunology - metabolism | - |
dc.subject.mesh | Signal Transduction | - |
dc.subject.mesh | T-Lymphocytes, Regulatory - immunology - metabolism | - |
dc.subject.mesh | Toll-Like Receptor 5 - antagonists and inhibitors - metabolism | - |
dc.title | TLR5 signaling enhances the proliferation of human allogeneic CD40-activated B cell induced CD4hiCD25+ regulatory T cells | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chan, PL: pandacha@hku.hk | en_US |
dc.identifier.email | Zheng, J: teddy629@hku.hk | en_US |
dc.identifier.email | Liu, Y: yinpingl@hku.hk | en_US |
dc.identifier.email | Mao, H: hwmau@hku.hk | en_US |
dc.identifier.email | Lau, YL: lauylung@hkucc.hku.hk | en_US |
dc.identifier.email | Tu, W: wwtu@yahoo.com | - |
dc.identifier.authority | Liu, Y=rp00269 | en_US |
dc.identifier.authority | Mao, H=rp01595 | en_US |
dc.identifier.authority | Lau, YL=rp00361 | en_US |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1371/journal.pone.0067969 | - |
dc.identifier.pmid | 23844139 | - |
dc.identifier.pmcid | PMC3700901 | - |
dc.identifier.scopus | eid_2-s2.0-84879776656 | - |
dc.identifier.hkuros | 225525 | en_US |
dc.identifier.hkuros | 221461 | - |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 7 | - |
dc.identifier.spage | e67969 | - |
dc.identifier.epage | e67969 | - |
dc.identifier.isi | WOS:000321733000121 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1932-6203 | - |