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Article: TLR5 signaling enhances the proliferation of human allogeneic CD40-activated B cell induced CD4hiCD25+ regulatory T cells

TitleTLR5 signaling enhances the proliferation of human allogeneic CD40-activated B cell induced CD4hiCD25+ regulatory T cells
Authors
Issue Date2013
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2013, v. 8 n. 7, p. e67969 How to Cite?
AbstractAlthough diverse functions of different toll-like receptors (TLR) on human natural regulatory T cells have been demonstrated recently, the role of TLR-related signals on human induced regulatory T cells remain elusive. Previously our group developed an ex vivo high-efficient system in generating human alloantigen-specific CD4(hi)CD25(+) regulatory T cells from naive CD4(+)CD25(-) T cells using allogeneic CD40-activated B cells as stimulators. In this study, we investigated the role of TLR5-related signals on the generation and function of these novel CD4(hi)CD25(+) regulatory T cells. It was found that induced CD4(hi)CD25(+) regulatory T cells expressed an up-regulated level of TLR5 compared to their precursors. The blockade of TLR5 using anti-TLR5 antibodies during the co-culture decreased CD4(hi)CD25(+) regulatory T cells proliferation by induction of S phase arrest. The S phase arrest was associated with reduced ERK1/2 phosphorylation. However, TLR5 blockade did not decrease the CTLA-4, GITR and FOXP3 expressions, and the suppressive function of CD4(hi)CD25(+) regulatory T cells. In conclusion, we discovered a novel function of TLR5-related signaling in enhancing the proliferation of CD4(hi)CD25(+) regulatory T cells by promoting S phase progress but not involved in the suppressive function of human CD40-activated B cell-induced CD4(hi)CD25(+) regulatory T cells, suggesting a novel role of TLR5-related signals in the generation of induced regulatory T cells.
Persistent Identifierhttp://hdl.handle.net/10722/191460
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, PLen_US
dc.contributor.authorZheng, Jen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorLam, KTen_US
dc.contributor.authorXiang, Zen_US
dc.contributor.authorMao, H-
dc.contributor.authorLiu, Y-
dc.contributor.authorQin, G-
dc.contributor.authorLau, YL-
dc.contributor.authorTu, W-
dc.date.accessioned2013-10-15T07:01:21Z-
dc.date.available2013-10-15T07:01:21Z-
dc.date.issued2013en_US
dc.identifier.citationPlos One, 2013, v. 8 n. 7, p. e67969en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/191460-
dc.description.abstractAlthough diverse functions of different toll-like receptors (TLR) on human natural regulatory T cells have been demonstrated recently, the role of TLR-related signals on human induced regulatory T cells remain elusive. Previously our group developed an ex vivo high-efficient system in generating human alloantigen-specific CD4(hi)CD25(+) regulatory T cells from naive CD4(+)CD25(-) T cells using allogeneic CD40-activated B cells as stimulators. In this study, we investigated the role of TLR5-related signals on the generation and function of these novel CD4(hi)CD25(+) regulatory T cells. It was found that induced CD4(hi)CD25(+) regulatory T cells expressed an up-regulated level of TLR5 compared to their precursors. The blockade of TLR5 using anti-TLR5 antibodies during the co-culture decreased CD4(hi)CD25(+) regulatory T cells proliferation by induction of S phase arrest. The S phase arrest was associated with reduced ERK1/2 phosphorylation. However, TLR5 blockade did not decrease the CTLA-4, GITR and FOXP3 expressions, and the suppressive function of CD4(hi)CD25(+) regulatory T cells. In conclusion, we discovered a novel function of TLR5-related signaling in enhancing the proliferation of CD4(hi)CD25(+) regulatory T cells by promoting S phase progress but not involved in the suppressive function of human CD40-activated B cell-induced CD4(hi)CD25(+) regulatory T cells, suggesting a novel role of TLR5-related signals in the generation of induced regulatory T cells.-
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPlos Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAntigens, CD40 - immunology-
dc.subject.meshB-Lymphocytes - immunology - metabolism-
dc.subject.meshSignal Transduction-
dc.subject.meshT-Lymphocytes, Regulatory - immunology - metabolism-
dc.subject.meshToll-Like Receptor 5 - antagonists and inhibitors - metabolism-
dc.titleTLR5 signaling enhances the proliferation of human allogeneic CD40-activated B cell induced CD4hiCD25+ regulatory T cellsen_US
dc.typeArticleen_US
dc.identifier.emailChan, PL: pandacha@hku.hken_US
dc.identifier.emailZheng, J: teddy629@hku.hken_US
dc.identifier.emailLiu, Y: yinpingl@hku.hken_US
dc.identifier.emailMao, H: hwmau@hku.hken_US
dc.identifier.emailLau, YL: lauylung@hkucc.hku.hken_US
dc.identifier.emailTu, W: wwtu@yahoo.com-
dc.identifier.authorityLiu, Y=rp00269en_US
dc.identifier.authorityMao, H=rp01595en_US
dc.identifier.authorityLau, YL=rp00361en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0067969-
dc.identifier.pmid23844139-
dc.identifier.pmcidPMC3700901-
dc.identifier.scopuseid_2-s2.0-84879776656-
dc.identifier.hkuros225525en_US
dc.identifier.hkuros221461-
dc.identifier.volume8-
dc.identifier.issue7-
dc.identifier.spagee67969-
dc.identifier.epagee67969-
dc.identifier.isiWOS:000321733000121-
dc.publisher.placeUnited States-

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