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Article: Evaluation Of The Lightcycler Methicillin-resistant Staphylococcus Aureus (mrsa) Advanced Test For Detection Of Mrsa Nasal Colonication

TitleEvaluation Of The Lightcycler Methicillin-resistant Staphylococcus Aureus (mrsa) Advanced Test For Detection Of Mrsa Nasal Colonication
Authors
Issue Date2013
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jcm.asm.org/
Citation
Journal of Clinical Microbiology, 2013, v. 51 n. 9, p. 2869-2874 How to Cite?
AbstractRapid detection of methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization is crucial for the prevention and control of MRSA infections in health care settings. The LightCycler MRSA Advanced Test (Roche Diagnostics) is a commercially available real-time PCR assay for direct detection of MRSA nasal colonization by targeting of the staphylococcal cassette chromosome mec (SCCmec)-orfX junction. The diagnostic performance of the assay was compared with that of ChromID MRSA agar (bioMérieux) culture and an in-house duplex real-time PCR assay. Among 1,246 nasal swab specimens collected from 2 general hospitals in Hong Kong, 174 (14%) were considered true positive for MRSA. Chromogenic culture and the in-house real-time PCR assay identified 147 (84.5%) and 133 (76.4%) true-positive cases with specificities of 100% and 98.6%, respectively. Based on the target melting temperature (Tm) values (57.0 to 62.0°C) defined by the manufacturer, the LightCycler MRSA Advanced Test identified only 85 (48.9%) true-positive specimens. Interestingly, an additional 60 (34.5%) true-positive specimens were detected despite atypical Tm values of 55°C, providing overall sensitivity and specificity values of 83.3% and 99%, respectively. Among isolates with Tm values of 55°C, most were typed as clonal complex 45 (CC45). By sequence analysis of the SCCmec-orfX junction, characteristic single-nucleotide polymorphisms (SNPs) were identified only in isolates with Tm values of 55°C and not in those with typical Tm values. It is conceivable that those SNPs were located inside the target region of the proprietary hybridization probes, which resulted in a Tm shift in the melting curve analysis. Our study highlights the importance of a global evaluation of commercial kits so that the interpretation algorithm covers different lineages of MRSA clones prevalent in various geographical regions. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
Descriptioneid_2-s2.0-84882786765 ; WOS:000323214200010
Persistent Identifierhttp://hdl.handle.net/10722/191432
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYam, WC-
dc.contributor.authorSiu, GKH-
dc.contributor.authorHo, PL-
dc.contributor.authorNG, TK-
dc.contributor.authorQue, TL-
dc.contributor.authorYip, KT-
dc.contributor.authorFOK, CP-
dc.contributor.authorChen, JHK-
dc.contributor.authorCheng, VCC-
dc.contributor.authorYuen, KY-
dc.date.accessioned2013-10-15T06:59:31Z-
dc.date.available2013-10-15T06:59:31Z-
dc.date.issued2013-
dc.identifier.citationJournal of Clinical Microbiology, 2013, v. 51 n. 9, p. 2869-2874-
dc.identifier.issn0095-1137-
dc.identifier.urihttp://hdl.handle.net/10722/191432-
dc.descriptioneid_2-s2.0-84882786765 ; WOS:000323214200010-
dc.description.abstractRapid detection of methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization is crucial for the prevention and control of MRSA infections in health care settings. The LightCycler MRSA Advanced Test (Roche Diagnostics) is a commercially available real-time PCR assay for direct detection of MRSA nasal colonization by targeting of the staphylococcal cassette chromosome mec (SCCmec)-orfX junction. The diagnostic performance of the assay was compared with that of ChromID MRSA agar (bioMérieux) culture and an in-house duplex real-time PCR assay. Among 1,246 nasal swab specimens collected from 2 general hospitals in Hong Kong, 174 (14%) were considered true positive for MRSA. Chromogenic culture and the in-house real-time PCR assay identified 147 (84.5%) and 133 (76.4%) true-positive cases with specificities of 100% and 98.6%, respectively. Based on the target melting temperature (Tm) values (57.0 to 62.0°C) defined by the manufacturer, the LightCycler MRSA Advanced Test identified only 85 (48.9%) true-positive specimens. Interestingly, an additional 60 (34.5%) true-positive specimens were detected despite atypical Tm values of 55°C, providing overall sensitivity and specificity values of 83.3% and 99%, respectively. Among isolates with Tm values of 55°C, most were typed as clonal complex 45 (CC45). By sequence analysis of the SCCmec-orfX junction, characteristic single-nucleotide polymorphisms (SNPs) were identified only in isolates with Tm values of 55°C and not in those with typical Tm values. It is conceivable that those SNPs were located inside the target region of the proprietary hybridization probes, which resulted in a Tm shift in the melting curve analysis. Our study highlights the importance of a global evaluation of commercial kits so that the interpretation algorithm covers different lineages of MRSA clones prevalent in various geographical regions. Copyright © 2013, American Society for Microbiology. All Rights Reserved.-
dc.languageeng-
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jcm.asm.org/-
dc.relation.ispartofJournal of Clinical Microbiology-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.-
dc.titleEvaluation Of The Lightcycler Methicillin-resistant Staphylococcus Aureus (mrsa) Advanced Test For Detection Of Mrsa Nasal Colonication-
dc.typeArticle-
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hk-
dc.identifier.emailSiu, GKH: gilman06@hkucc.hku.hk-
dc.identifier.emailHo, PL: plho@hkucc.hku.hk-
dc.identifier.emailChen, JHK: jonchk@hku.hk-
dc.identifier.emailCheng, VCC: vcccheng@hkucc.hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.authorityYam, WC=rp00313-
dc.identifier.authorityHo, PL=rp00406-
dc.identifier.authorityYuen, KY=rp00366-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JCM.00488-13-
dc.identifier.pmid23784133-
dc.identifier.pmcidPMC3754679-
dc.identifier.hkuros225739-
dc.identifier.volume51-
dc.identifier.issue9-
dc.identifier.spage2869-
dc.identifier.epage2874-
dc.identifier.isiWOS:000323214200010-
dc.publisher.placeUnited States-

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