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Conference Paper: Fabrication of uniform multi-compartment particles using microfludic electrospray technology for cell co-culture studies

TitleFabrication of uniform multi-compartment particles using microfludic electrospray technology for cell co-culture studies
Authors
KeywordsCell-cell interaction
Extracellular matrices
High field strengths
Hydrogel particles
Mechanical environment
Monodisperse droplets
Multiple compartments
Significant mixing
Issue Date2013
PublisherAmerican Institute of Physics. The Journal's web site is located at http://bmf.aip.org
Citation
The 3rd European Conference on Microfluidics (μFlu'12), Heidelberg, Germany, 3-5 December 2012. In Biomicrofluidics, 2013, v. 7 n. 4, p. 044117-1 - 044117-8 How to Cite?
AbstractIn this work, we demonstrate a robust and reliable approach to fabricate multi-compartment particles for cell co-culture studies. By taking advantage of the laminar flow within our microfluidic nozzle, multiple parallel streams of liquids flow towards the nozzle without significant mixing. Afterwards, the multiple parallel streams merge into a single stream, which is sprayed into air, forming monodisperse droplets under an electric field with a high field strength. The resultant multi-compartment droplets are subsequently cross-linked in a calcium chloride solution to form calcium alginate micro-particles with multiple compartments. Each compartment of the particles can be used for encapsulating different types of cells or biological cell factors. These hydrogel particles with cross-linked alginate chains show similarity in the physical and mechanical environment as the extracellular matrix of biological cells. Thus, the multi-compartment particles provide a promising platform for cell studies and co-culture of different cells. In our study, cells are encapsulated in the multi-compartment particles and the viability of cells is quantified using a fluorescence microscope after the cells are stained for a live/dead assay. The high cell viability after encapsulation indicates the cytocompatibility and feasibility of our technique. Our multi-compartment particles have great potential as a platform for studying cell-cell interactions as well as interactions of cells with extracellular factors. © 2013 AIP Publishing LLC.
DescriptionFootnote in article: Paper submitted as part of the 3rd European Conference on Microfluidics ... 2012
Persistent Identifierhttp://hdl.handle.net/10722/190009
ISSN
2015 Impact Factor: 2.708
2015 SCImago Journal Rankings: 0.794
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, Zen_US
dc.contributor.authorShum, HCen_US
dc.date.accessioned2013-09-17T15:04:37Z-
dc.date.available2013-09-17T15:04:37Z-
dc.date.issued2013en_US
dc.identifier.citationThe 3rd European Conference on Microfluidics (μFlu'12), Heidelberg, Germany, 3-5 December 2012. In Biomicrofluidics, 2013, v. 7 n. 4, p. 044117-1 - 044117-8en_US
dc.identifier.issn1932-1058-
dc.identifier.urihttp://hdl.handle.net/10722/190009-
dc.descriptionFootnote in article: Paper submitted as part of the 3rd European Conference on Microfluidics ... 2012-
dc.description.abstractIn this work, we demonstrate a robust and reliable approach to fabricate multi-compartment particles for cell co-culture studies. By taking advantage of the laminar flow within our microfluidic nozzle, multiple parallel streams of liquids flow towards the nozzle without significant mixing. Afterwards, the multiple parallel streams merge into a single stream, which is sprayed into air, forming monodisperse droplets under an electric field with a high field strength. The resultant multi-compartment droplets are subsequently cross-linked in a calcium chloride solution to form calcium alginate micro-particles with multiple compartments. Each compartment of the particles can be used for encapsulating different types of cells or biological cell factors. These hydrogel particles with cross-linked alginate chains show similarity in the physical and mechanical environment as the extracellular matrix of biological cells. Thus, the multi-compartment particles provide a promising platform for cell studies and co-culture of different cells. In our study, cells are encapsulated in the multi-compartment particles and the viability of cells is quantified using a fluorescence microscope after the cells are stained for a live/dead assay. The high cell viability after encapsulation indicates the cytocompatibility and feasibility of our technique. Our multi-compartment particles have great potential as a platform for studying cell-cell interactions as well as interactions of cells with extracellular factors. © 2013 AIP Publishing LLC.-
dc.languageengen_US
dc.publisherAmerican Institute of Physics. The Journal's web site is located at http://bmf.aip.org-
dc.relation.ispartofBiomicrofluidicsen_US
dc.rightsBiomicrofluidics. Copyright © American Institute of Physics.-
dc.rightsCopyright 2013 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Biomicrofluidics, 2013, v. 7 n. 4, p. 044117-1 - 044117-8 and may be found at http://dx.doi.org/10.1063/1.4817769.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCell-cell interaction-
dc.subjectExtracellular matrices-
dc.subjectHigh field strengths-
dc.subjectHydrogel particles-
dc.subjectMechanical environment-
dc.subjectMonodisperse droplets-
dc.subjectMultiple compartments-
dc.subjectSignificant mixing-
dc.titleFabrication of uniform multi-compartment particles using microfludic electrospray technology for cell co-culture studiesen_US
dc.typeConference_Paperen_US
dc.identifier.emailShum, HC: ashum@hku.hken_US
dc.identifier.authorityShum, HC=rp01439en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1063/1.4817769-
dc.identifier.pmid24404050-
dc.identifier.pmcidPMC3758358-
dc.identifier.scopuseid_2-s2.0-84883311013-
dc.identifier.hkuros223197en_US
dc.identifier.hkuros223302-
dc.identifier.volume7-
dc.identifier.issue4-
dc.identifier.spage044117-1-
dc.identifier.epage044117-8-
dc.identifier.isiWOS:000323907600019-
dc.publisher.placeUnited States-
dc.customcontrol.immutablesml 140102-

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