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Conference Paper: Real-time imaging of plasma membrane dynamics in sonoporation: from perforation to recovery

TitleReal-time imaging of plasma membrane dynamics in sonoporation: from perforation to recovery
Authors
Issue Date2013
PublisherIEEE.
Citation
The 2013 IEEE-Internatioanl Ultrasonics Symposium (IUS), Joint UFFC, EFTF and PFM Symposium, Prague, Czech Republic, 21-25 July 2013. In IUS Abstracts, 2013, p. 410-411 How to Cite?
AbstractBACKGROUND, MOTIVATION AND OBJECTIVE: To properly harness sonoporation for therapeutic applications, it is unarguably vital to characterize the fundamental biophysical processes involved. Of particular relevance are two membrane-level processes that epitomize the notion of sonoporation: 1) how membrane perforation is induced by ultrasound-microbubble interactions, and 2) how the membrane remodels itself following an episode of sonoporation. Acquiring direct observations of these processes is however not a straightforward task (ironically, these membrane-level events have yet to be convincingly demonstrated in-situ). In this investigation, our aim is to acquire the first series of direct evidence on the time course of membrane perforation and recovery in sonoporation. In particular, we seek to unravel the time-varying surface topography of sonoporated cell membrane in-situ. STATEMENT OF CONTRIBUTION/METHODS: A real-time imaging platform for monitoring of cell-microbubble interactions was first developed, and it was a composite system that coupled a 1 MHz ultrasound module to a laser scanning confocal microscope. A nose-cone shaped waveguide (1” diameter, 7.5 cm height) was devised to align the ultrasound beam focus to the microscope’s imaging plane. This waveguide was angled at 45 deg. with respect …
DescriptionSession IUS1-K3: Metrology and sonoporation: abstract no. IUS1-K3-4
The Conference program & abstracts' website is located at http://www.ewh.ieee.org/conf/uffc/2013/
Persistent Identifierhttp://hdl.handle.net/10722/189842

 

DC FieldValueLanguage
dc.contributor.authorHu, Yen_US
dc.contributor.authorWan, JMFen_US
dc.contributor.authorYu, ACHen_US
dc.date.accessioned2013-09-17T15:00:49Z-
dc.date.available2013-09-17T15:00:49Z-
dc.date.issued2013en_US
dc.identifier.citationThe 2013 IEEE-Internatioanl Ultrasonics Symposium (IUS), Joint UFFC, EFTF and PFM Symposium, Prague, Czech Republic, 21-25 July 2013. In IUS Abstracts, 2013, p. 410-411en_US
dc.identifier.urihttp://hdl.handle.net/10722/189842-
dc.descriptionSession IUS1-K3: Metrology and sonoporation: abstract no. IUS1-K3-4-
dc.descriptionThe Conference program & abstracts' website is located at http://www.ewh.ieee.org/conf/uffc/2013/-
dc.description.abstractBACKGROUND, MOTIVATION AND OBJECTIVE: To properly harness sonoporation for therapeutic applications, it is unarguably vital to characterize the fundamental biophysical processes involved. Of particular relevance are two membrane-level processes that epitomize the notion of sonoporation: 1) how membrane perforation is induced by ultrasound-microbubble interactions, and 2) how the membrane remodels itself following an episode of sonoporation. Acquiring direct observations of these processes is however not a straightforward task (ironically, these membrane-level events have yet to be convincingly demonstrated in-situ). In this investigation, our aim is to acquire the first series of direct evidence on the time course of membrane perforation and recovery in sonoporation. In particular, we seek to unravel the time-varying surface topography of sonoporated cell membrane in-situ. STATEMENT OF CONTRIBUTION/METHODS: A real-time imaging platform for monitoring of cell-microbubble interactions was first developed, and it was a composite system that coupled a 1 MHz ultrasound module to a laser scanning confocal microscope. A nose-cone shaped waveguide (1” diameter, 7.5 cm height) was devised to align the ultrasound beam focus to the microscope’s imaging plane. This waveguide was angled at 45 deg. with respect …-
dc.languageengen_US
dc.publisherIEEE.-
dc.relation.ispartofIEEE-IUS, Joint UFFC, EFTF and PFM Symposium 2013en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleReal-time imaging of plasma membrane dynamics in sonoporation: from perforation to recoveryen_US
dc.typeConference_Paperen_US
dc.identifier.emailWan, JMF: jmfwan@hku.hken_US
dc.identifier.emailYu, ACH: alfred.yu@hku.hken_US
dc.identifier.authorityWan, JMF=rp00798en_US
dc.identifier.authorityYu, ACH=rp00657en_US
dc.description.naturepostprint-
dc.identifier.hkuros222147en_US
dc.identifier.spage410-
dc.identifier.epage411-

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