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Conference Paper: Ultrasound as a repulsive cue for neuronal development: real-time morphological observations in-vitro

TitleUltrasound as a repulsive cue for neuronal development: real-time morphological observations in-vitro
Authors
Issue Date2013
PublisherIEEE.
Citation
The 2013 IEEE-Internatioanl Ultrasonics Symposium (IUS), Joint UFFC, EFTF and PFM Symposium, Prague, Czech Republic, 21-25 July 2013. In IUS Abstracts, 2013, p. 95-96 How to Cite?
AbstractBACKGROUND, MOTIVATION AND OBJECTIVE: A core event in neuronal development is the outgrowth of neurite branches from a neuron’s cell body. It is known that, by presenting physical or chemical cues to the extracellular environment, neuronal development can potentially be modulated. In this work, we seek to investigate the use of ultrasound in modifying neuronal development behavior. We hypothesize that ultrasound, when applied at low intensity and pulsed settings, can possibly serve as a physical cue that operates like mechanical stimulation. STATEMENT OF CONTRIBUTION/METHODS: Our study was carried out on a customized platform that allows real-time imaging of neuronal morphology. Pulsed ultrasound (1 MHz frequency, 20 or 100 cycle pulse lengths, 500 or 2500 Hz PRF) was applied through a waveguide at a 45 degree angle with respect to the microscope’s field of view, which is aligned to the center of a 100mm polystyrene cell dish. Three different peak acoustic pressures (measured in-situ) were used: 0.13, 0.40, 0.84 MPa (correspond to SPTA intensities of up to 1.168 W/cm2 for our given pulsing parameters). In this work, N2a mouse neuroblastoma was used as the …
DescriptionSession IUS1-PA4: Bioeffects and metrology: abstract no. IUS1-PA4-6
The Conference program & abstracts' website is located at http://www.ewh.ieee.org/conf/uffc/2013/
Persistent Identifierhttp://hdl.handle.net/10722/189841

 

DC FieldValueLanguage
dc.contributor.authorHu, Yen_US
dc.contributor.authorZhong, Wen_US
dc.contributor.authorWan, JMFen_US
dc.contributor.authorYu, ACHen_US
dc.date.accessioned2013-09-17T15:00:49Z-
dc.date.available2013-09-17T15:00:49Z-
dc.date.issued2013en_US
dc.identifier.citationThe 2013 IEEE-Internatioanl Ultrasonics Symposium (IUS), Joint UFFC, EFTF and PFM Symposium, Prague, Czech Republic, 21-25 July 2013. In IUS Abstracts, 2013, p. 95-96en_US
dc.identifier.urihttp://hdl.handle.net/10722/189841-
dc.descriptionSession IUS1-PA4: Bioeffects and metrology: abstract no. IUS1-PA4-6-
dc.descriptionThe Conference program & abstracts' website is located at http://www.ewh.ieee.org/conf/uffc/2013/-
dc.description.abstractBACKGROUND, MOTIVATION AND OBJECTIVE: A core event in neuronal development is the outgrowth of neurite branches from a neuron’s cell body. It is known that, by presenting physical or chemical cues to the extracellular environment, neuronal development can potentially be modulated. In this work, we seek to investigate the use of ultrasound in modifying neuronal development behavior. We hypothesize that ultrasound, when applied at low intensity and pulsed settings, can possibly serve as a physical cue that operates like mechanical stimulation. STATEMENT OF CONTRIBUTION/METHODS: Our study was carried out on a customized platform that allows real-time imaging of neuronal morphology. Pulsed ultrasound (1 MHz frequency, 20 or 100 cycle pulse lengths, 500 or 2500 Hz PRF) was applied through a waveguide at a 45 degree angle with respect to the microscope’s field of view, which is aligned to the center of a 100mm polystyrene cell dish. Three different peak acoustic pressures (measured in-situ) were used: 0.13, 0.40, 0.84 MPa (correspond to SPTA intensities of up to 1.168 W/cm2 for our given pulsing parameters). In this work, N2a mouse neuroblastoma was used as the …-
dc.languageengen_US
dc.publisherIEEE.-
dc.relation.ispartofIEEE-IUS, Joint UFFC, EFTF and PFM Symposium 2013en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleUltrasound as a repulsive cue for neuronal development: real-time morphological observations in-vitroen_US
dc.typeConference_Paperen_US
dc.identifier.emailWan, JMF: jmfwan@hku.hken_US
dc.identifier.emailYu, ACH: alfred.yu@hku.hken_US
dc.identifier.authorityWan, JMF=rp00798en_US
dc.identifier.authorityYu, ACH=rp00657en_US
dc.description.naturepostprint-
dc.identifier.hkuros222146en_US
dc.identifier.spage95-
dc.identifier.epage96-

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