File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Cost-effective approaches for high-resolution bioimaging by time-stretched confocal microscopy at 1um

TitleCost-effective approaches for high-resolution bioimaging by time-stretched confocal microscopy at 1um
Authors
Issue Date2012
PublisherS P I E - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xml?WT.svl=mddp2
Citation
Conference 8553 - Optics in Health Care and Biomedical Optics V, Beijing, China, 5 November 2012. In Proceedings of SPIE, 2012, v. 8553, p. article no. 85531P How to Cite?
AbstractOptical imaging based on time-stretch process has recently been proven as a powerful tool for delivering ultra-high frame rate (< 1MHz) which is not achievable by the conventional image sensors. Together with the capability of optical image amplification for overcoming the trade-off between detection sensitivity and speed, this new imaging modality is particularly valuable in high-throughput biomedical diagnostic practice, e.g. imaging flow cytometry. The ultra-high frame rate in time-stretch imaging is attained by two key enabling elements: dispersive fiber providing the time-stretch process via group-velocity-dispersion (GVD), and electronic digitizer. It is well-known that many biophotonic applications favor the spectral window of 1μm. However, reasonably high GVD (< 0.1 ns/nm) in this range can only be achieved by using specialty single-mode fiber (SMF) at 1μm. Moreover, the ultrafast detection has to rely on the state-of- the-art digitizer with significantly wide-bandwidth and high sampling rate (e.g. <10 GHz, <40 GS/s). These stringent requirements imply the prohibitively high-cost of the system and hinder its practical use in biomedical diagnostics. We here demonstrate two cost-effective approaches for realizing time-stretch confocal microscopy at 1μm: (i) using the standard telecommunication SMF (e.g. SMF28) to act as a few-mode fiber (FMF) at 1μm for the time-stretch process, and (ii) implementing the pixel super-resolution (SR) algorithm to restore the high-resolution (HR) image when using a lower-bandwidth digitizer. By using a FMF (with a GVD of 0.15ns/nm) and a modified pixel-SR algorithm, we can achieve time-stretch confocal microscopy at 1μm with cellular resolution ( 3μm) at a frame rate 1 MHz.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
DescriptionSession: Optics Imaging Algorithms and Analysis II
Persistent Identifierhttp://hdl.handle.net/10722/189812
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, TWen_US
dc.contributor.authorQiu, Yen_US
dc.contributor.authorLau, KSen_US
dc.contributor.authorXu, Jen_US
dc.contributor.authorChan, CSen_US
dc.contributor.authorWong, KKYen_US
dc.contributor.authorTsia, KKMen_US
dc.date.accessioned2013-09-17T15:00:39Z-
dc.date.available2013-09-17T15:00:39Z-
dc.date.issued2012en_US
dc.identifier.citationConference 8553 - Optics in Health Care and Biomedical Optics V, Beijing, China, 5 November 2012. In Proceedings of SPIE, 2012, v. 8553, p. article no. 85531Pen_US
dc.identifier.issn0277-786X-
dc.identifier.urihttp://hdl.handle.net/10722/189812-
dc.descriptionSession: Optics Imaging Algorithms and Analysis II-
dc.description.abstractOptical imaging based on time-stretch process has recently been proven as a powerful tool for delivering ultra-high frame rate (< 1MHz) which is not achievable by the conventional image sensors. Together with the capability of optical image amplification for overcoming the trade-off between detection sensitivity and speed, this new imaging modality is particularly valuable in high-throughput biomedical diagnostic practice, e.g. imaging flow cytometry. The ultra-high frame rate in time-stretch imaging is attained by two key enabling elements: dispersive fiber providing the time-stretch process via group-velocity-dispersion (GVD), and electronic digitizer. It is well-known that many biophotonic applications favor the spectral window of 1μm. However, reasonably high GVD (< 0.1 ns/nm) in this range can only be achieved by using specialty single-mode fiber (SMF) at 1μm. Moreover, the ultrafast detection has to rely on the state-of- the-art digitizer with significantly wide-bandwidth and high sampling rate (e.g. <10 GHz, <40 GS/s). These stringent requirements imply the prohibitively high-cost of the system and hinder its practical use in biomedical diagnostics. We here demonstrate two cost-effective approaches for realizing time-stretch confocal microscopy at 1μm: (i) using the standard telecommunication SMF (e.g. SMF28) to act as a few-mode fiber (FMF) at 1μm for the time-stretch process, and (ii) implementing the pixel super-resolution (SR) algorithm to restore the high-resolution (HR) image when using a lower-bandwidth digitizer. By using a FMF (with a GVD of 0.15ns/nm) and a modified pixel-SR algorithm, we can achieve time-stretch confocal microscopy at 1μm with cellular resolution ( 3μm) at a frame rate 1 MHz.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.-
dc.languageengen_US
dc.publisherS P I E - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xml?WT.svl=mddp2-
dc.relation.ispartofProceedings of SPIE - International Society for Optical Engineeringen_US
dc.rightsProceedings of SPIE - International Society for Optical Engineering. Copyright © S P I E - International Society for Optical Engineering.-
dc.rightsCopyright notice format: Copyright 2012 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleCost-effective approaches for high-resolution bioimaging by time-stretched confocal microscopy at 1umen_US
dc.typeConference_Paperen_US
dc.identifier.emailWong, KKY: kywong04@hkucc.hku.hken_US
dc.identifier.emailTsia, KKM: tsia@hku.hken_US
dc.identifier.authorityWong, KKY=rp00189en_US
dc.identifier.authorityTsia, KKM=rp01389en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1117/12.999833-
dc.identifier.hkuros221027en_US
dc.identifier.volume8553-
dc.identifier.spagearticle no. 85531P-
dc.identifier.epagearticle no. 85531P-
dc.identifier.isiWOS:000322824000036-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats