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Conference Paper: Cost-effective approaches for high-resolution bioimaging by time-stretched confocal microscopy at 1um

TitleCost-effective approaches for high-resolution bioimaging by time-stretched confocal microscopy at 1um
Authors
Issue Date2012
PublisherS P I E - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xml?WT.svl=mddp2
Citation
Conference 8553 - Optics in Health Care and Biomedical Optics V, Beijing, China, 5 November 2012. In Proceedings of SPIE, 2012, v. 8553, p. article no. 85531P How to Cite?
AbstractOptical imaging based on time-stretch process has recently been proven as a powerful tool for delivering ultra-high frame rate (< 1MHz) which is not achievable by the conventional image sensors. Together with the capability of optical image amplification for overcoming the trade-off between detection sensitivity and speed, this new imaging modality is particularly valuable in high-throughput biomedical diagnostic practice, e.g. imaging flow cytometry. The ultra-high frame rate in time-stretch imaging is attained by two key enabling elements: dispersive fiber providing the time-stretch process via group-velocity-dispersion (GVD), and electronic digitizer. It is well-known that many biophotonic applications favor the spectral window of 1μm. However, reasonably high GVD (< 0.1 ns/nm) in this range can only be achieved by using specialty single-mode fiber (SMF) at 1μm. Moreover, the ultrafast detection has to rely on the state-of- the-art digitizer with significantly wide-bandwidth and high sampling rate (e.g. <10 GHz, <40 GS/s). These stringent requirements imply the prohibitively high-cost of the system and hinder its practical use in biomedical diagnostics. We here demonstrate two cost-effective approaches for realizing time-stretch confocal microscopy at 1μm: (i) using the standard telecommunication SMF (e.g. SMF28) to act as a few-mode fiber (FMF) at 1μm for the time-stretch process, and (ii) implementing the pixel super-resolution (SR) algorithm to restore the high-resolution (HR) image when using a lower-bandwidth digitizer. By using a FMF (with a GVD of 0.15ns/nm) and a modified pixel-SR algorithm, we can achieve time-stretch confocal microscopy at 1μm with cellular resolution ( 3μm) at a frame rate 1 MHz.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
DescriptionSession: Optics Imaging Algorithms and Analysis II
Persistent Identifierhttp://hdl.handle.net/10722/189812
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, TWen_US
dc.contributor.authorQiu, Yen_US
dc.contributor.authorLau, KSen_US
dc.contributor.authorXu, Jen_US
dc.contributor.authorChan, CSen_US
dc.contributor.authorWong, KKYen_US
dc.contributor.authorTsia, KKMen_US
dc.date.accessioned2013-09-17T15:00:39Z-
dc.date.available2013-09-17T15:00:39Z-
dc.date.issued2012en_US
dc.identifier.citationConference 8553 - Optics in Health Care and Biomedical Optics V, Beijing, China, 5 November 2012. In Proceedings of SPIE, 2012, v. 8553, p. article no. 85531Pen_US
dc.identifier.issn0277-786X-
dc.identifier.urihttp://hdl.handle.net/10722/189812-
dc.descriptionSession: Optics Imaging Algorithms and Analysis II-
dc.description.abstractOptical imaging based on time-stretch process has recently been proven as a powerful tool for delivering ultra-high frame rate (< 1MHz) which is not achievable by the conventional image sensors. Together with the capability of optical image amplification for overcoming the trade-off between detection sensitivity and speed, this new imaging modality is particularly valuable in high-throughput biomedical diagnostic practice, e.g. imaging flow cytometry. The ultra-high frame rate in time-stretch imaging is attained by two key enabling elements: dispersive fiber providing the time-stretch process via group-velocity-dispersion (GVD), and electronic digitizer. It is well-known that many biophotonic applications favor the spectral window of 1μm. However, reasonably high GVD (< 0.1 ns/nm) in this range can only be achieved by using specialty single-mode fiber (SMF) at 1μm. Moreover, the ultrafast detection has to rely on the state-of- the-art digitizer with significantly wide-bandwidth and high sampling rate (e.g. <10 GHz, <40 GS/s). These stringent requirements imply the prohibitively high-cost of the system and hinder its practical use in biomedical diagnostics. We here demonstrate two cost-effective approaches for realizing time-stretch confocal microscopy at 1μm: (i) using the standard telecommunication SMF (e.g. SMF28) to act as a few-mode fiber (FMF) at 1μm for the time-stretch process, and (ii) implementing the pixel super-resolution (SR) algorithm to restore the high-resolution (HR) image when using a lower-bandwidth digitizer. By using a FMF (with a GVD of 0.15ns/nm) and a modified pixel-SR algorithm, we can achieve time-stretch confocal microscopy at 1μm with cellular resolution ( 3μm) at a frame rate 1 MHz.© (2012) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.-
dc.languageengen_US
dc.publisherS P I E - International Society for Optical Engineering. The Journal's web site is located at http://spie.org/x1848.xml?WT.svl=mddp2-
dc.relation.ispartofProceedings of SPIE - International Society for Optical Engineeringen_US
dc.rightsCopyright 2012 Society of Photo‑Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited. This article is available online at https://doi.org/10.1117/12.999833-
dc.titleCost-effective approaches for high-resolution bioimaging by time-stretched confocal microscopy at 1umen_US
dc.typeConference_Paperen_US
dc.identifier.emailWong, KKY: kywong04@hkucc.hku.hken_US
dc.identifier.emailTsia, KKM: tsia@hku.hken_US
dc.identifier.authorityWong, KKY=rp00189en_US
dc.identifier.authorityTsia, KKM=rp01389en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1117/12.999833-
dc.identifier.hkuros221027en_US
dc.identifier.volume8553-
dc.identifier.spagearticle no. 85531P-
dc.identifier.epagearticle no. 85531P-
dc.identifier.isiWOS:000322824000036-
dc.publisher.placeUnited States-

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