File Download

Conference Paper: Adenoviral delivery of RNA decoys restores cellular proapoptotic protein PUMA expression by silencing Epstein-Barr virus-encoded miR-BART5 in nasopharyngeal carcinoma cells

TitleAdenoviral delivery of RNA decoys restores cellular proapoptotic protein PUMA expression by silencing Epstein-Barr virus-encoded miR-BART5 in nasopharyngeal carcinoma cells
Authors
Issue Date2012
PublisherICOHAD 2012.
Citation
The 2012 International Congress on Oncogenic Herpesviruses and Associated Diseases (ICOHAD 2012), Philadelphia, PA., 1-4 August 2012. In Conference Program, 2012, p. 38, abstract no. 3.17 How to Cite?
AbstractEpstein-Barr virus (EBV) encodes 48 mature microRNAs that play important roles in viral maintenance and promote host cell survival by regulating viral transcripts expression, inhibiting apoptosis or facilitating to evade cell immune surveillance. We have previously shown that EBV-encoded miR-BART5 targets and downregulates cellular pro-apoptotic protein p53-upregulated modulator of apoptosis (PUMA) to promote cellular survival of EBV-infected nasopharyngeal carcinoma (NPC) cells. Since compromising miR-BART5 might induce apoptosis of EBV-infected NPC cell, in this study we have established an adenoviral expression system to deliver anti-miR-BART5 decoys to NPC cells. The anti-miR-BART5 decoys comprised 6 tandem repeats of miR-BART5 binding sites and their expression was driven by EBVEBER2 promoter. They were designed to serve as a competitive inhibitor of miR-BART5 to reverse miR-BART5's inhibitory effects on PUMA in EBV-infected NPC cells. The RNA polymerase III-dependent EBER2 promoter is particularly strong in ...
DescriptionPoster Session 1 - Vaccines and Anti-Viral Therapeutics: no. 3.17
Persistent Identifierhttp://hdl.handle.net/10722/189763

 

DC FieldValueLanguage
dc.contributor.authorYuen, KSen_US
dc.contributor.authorTang, VHMen_US
dc.contributor.authorKok, KHen_US
dc.contributor.authorJin, Den_US
dc.date.accessioned2013-09-17T14:56:08Z-
dc.date.available2013-09-17T14:56:08Z-
dc.date.issued2012en_US
dc.identifier.citationThe 2012 International Congress on Oncogenic Herpesviruses and Associated Diseases (ICOHAD 2012), Philadelphia, PA., 1-4 August 2012. In Conference Program, 2012, p. 38, abstract no. 3.17en_US
dc.identifier.urihttp://hdl.handle.net/10722/189763-
dc.descriptionPoster Session 1 - Vaccines and Anti-Viral Therapeutics: no. 3.17-
dc.description.abstractEpstein-Barr virus (EBV) encodes 48 mature microRNAs that play important roles in viral maintenance and promote host cell survival by regulating viral transcripts expression, inhibiting apoptosis or facilitating to evade cell immune surveillance. We have previously shown that EBV-encoded miR-BART5 targets and downregulates cellular pro-apoptotic protein p53-upregulated modulator of apoptosis (PUMA) to promote cellular survival of EBV-infected nasopharyngeal carcinoma (NPC) cells. Since compromising miR-BART5 might induce apoptosis of EBV-infected NPC cell, in this study we have established an adenoviral expression system to deliver anti-miR-BART5 decoys to NPC cells. The anti-miR-BART5 decoys comprised 6 tandem repeats of miR-BART5 binding sites and their expression was driven by EBVEBER2 promoter. They were designed to serve as a competitive inhibitor of miR-BART5 to reverse miR-BART5's inhibitory effects on PUMA in EBV-infected NPC cells. The RNA polymerase III-dependent EBER2 promoter is particularly strong in ...-
dc.languageengen_US
dc.publisherICOHAD 2012.-
dc.relation.ispartofInternational Congress on Oncogenic Herpesviruses and Associated Diseases, ICOHAD 2012en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleAdenoviral delivery of RNA decoys restores cellular proapoptotic protein PUMA expression by silencing Epstein-Barr virus-encoded miR-BART5 in nasopharyngeal carcinoma cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailTang, VHM: tanghmv@hku.hken_US
dc.identifier.emailKok, KH: khkok@hku.hken_US
dc.identifier.emailJin, D: dyjin@hku.hken_US
dc.identifier.authorityKok, KH=rp01455en_US
dc.identifier.authorityJin, D=rp00452en_US
dc.description.naturepostprint-
dc.identifier.hkuros224777en_US
dc.identifier.spage38, abstract no. 3.17-
dc.identifier.epage38, abstract no. 3.17-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats