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Article: Evaluation of the correlation between focal adhesion kinase phosphorylation and cell adhesion force using ‘DEP’ technology

TitleEvaluation of the correlation between focal adhesion kinase phosphorylation and cell adhesion force using ‘DEP’ technology
Authors
Issue Date2012
PublisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.net/sensors
Citation
Sensors, 2012, v. 12 n. 5, p. 5951-5965 How to Cite?
AbstractDielectrophoresis (DEP) is the phenomenon in which a particle, such as a living cell, is polarized and moved by electrical gravity in a non-uniform electric field. In the present study, the DEP force is utilized to act on the cells to induce spatial movement for investigating the correlation between the cell adhesion force and activation level of focal adhesion kinase (FAK). The DEP force produced by the non-uniform electric field was used to measure the cell adhesion force of ECV304 cells, on type 1 collagen (COL1)- and fibronectin (FN)-coated polydimethylsiloxane (PDMS) membranes. For COL1-coating, ECV304 cells revealed weak and variable adhesion force (0.343-0.760 nN) in the first eight hours of incubation. Interestingly, the cell adhesion force of ECV304 at two and five hours of cultivation was significantly high and matched their FAK activation level. In comparison, ECV304 on FN-coated membrane had higher and more stable cell adhesion force (0.577-2.053 nN). FN coating intensified the cell adhesion force of ECV304 with culture time and similar outcome was present on the activation level of FAK. Therefore, this study demonstrated a relationship between cell adhesion force and FAK activation level that was dependent on the choice of the extracellular matrix (ECM) component. Subsequently, two tyrosine kinase inhibitors (AG18 and genistein) and one PI3K inhibitor (LY294002) were applied to study the influence of protein phosphorylation on the cell adhesion force. FAK plays an important role on cell attachment and DEP force measurement is a useful technique for studying cell adhesion.
Persistent Identifierhttp://hdl.handle.net/10722/189316
ISSN
2015 Impact Factor: 2.033
2015 SCImago Journal Rankings: 0.546
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorAy, Cen_US
dc.contributor.authorYeh, C-Cen_US
dc.contributor.authorHsu, M-Cen_US
dc.contributor.authorHurng, H-Yen_US
dc.contributor.authorKwok, PCLen_US
dc.contributor.authorChang, H-Ien_US
dc.date.accessioned2013-09-17T14:35:07Z-
dc.date.available2013-09-17T14:35:07Z-
dc.date.issued2012en_US
dc.identifier.citationSensors, 2012, v. 12 n. 5, p. 5951-5965en_US
dc.identifier.issn1424-8220en_US
dc.identifier.urihttp://hdl.handle.net/10722/189316-
dc.description.abstractDielectrophoresis (DEP) is the phenomenon in which a particle, such as a living cell, is polarized and moved by electrical gravity in a non-uniform electric field. In the present study, the DEP force is utilized to act on the cells to induce spatial movement for investigating the correlation between the cell adhesion force and activation level of focal adhesion kinase (FAK). The DEP force produced by the non-uniform electric field was used to measure the cell adhesion force of ECV304 cells, on type 1 collagen (COL1)- and fibronectin (FN)-coated polydimethylsiloxane (PDMS) membranes. For COL1-coating, ECV304 cells revealed weak and variable adhesion force (0.343-0.760 nN) in the first eight hours of incubation. Interestingly, the cell adhesion force of ECV304 at two and five hours of cultivation was significantly high and matched their FAK activation level. In comparison, ECV304 on FN-coated membrane had higher and more stable cell adhesion force (0.577-2.053 nN). FN coating intensified the cell adhesion force of ECV304 with culture time and similar outcome was present on the activation level of FAK. Therefore, this study demonstrated a relationship between cell adhesion force and FAK activation level that was dependent on the choice of the extracellular matrix (ECM) component. Subsequently, two tyrosine kinase inhibitors (AG18 and genistein) and one PI3K inhibitor (LY294002) were applied to study the influence of protein phosphorylation on the cell adhesion force. FAK plays an important role on cell attachment and DEP force measurement is a useful technique for studying cell adhesion.en_US
dc.languageengen_US
dc.publisherMolecular Diversity Preservation International. The Journal's web site is located at http://www.mdpi.net/sensorsen_US
dc.relation.ispartofSensorsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCell Adhesion - drug effectsen_US
dc.subject.meshElectrophoresisen_US
dc.subject.meshFocal Adhesion Protein-Tyrosine Kinases - antagonists and inhibitors - metabolismen_US
dc.subject.meshUrinary Bladder - cytology - enzymologyen_US
dc.titleEvaluation of the correlation between focal adhesion kinase phosphorylation and cell adhesion force using ‘DEP’ technologyen_US
dc.typeArticleen_US
dc.identifier.emailKwok, PCL: pclkwok@hku.hken_US
dc.identifier.authorityKwok, PCL=rp01540en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.3390/s120505951en_US
dc.identifier.pmid22778624en_US
dc.identifier.pmcidPMC3386723en_US
dc.identifier.hkuros222411en_US
dc.identifier.volume12en_US
dc.identifier.issue5en_US
dc.identifier.spage5951en_US
dc.identifier.epage5965en_US
dc.identifier.isiWOS:000304539200045-
dc.publisher.placeSwitzerlanden_US

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