File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Anti-hyperalgesic and anti-inflammatory effects of the modified Chinese herbal formula Huo Luo Xiao Ling Dan (HLXL) in rats

TitleAnti-hyperalgesic and anti-inflammatory effects of the modified Chinese herbal formula Huo Luo Xiao Ling Dan (HLXL) in rats
Authors
Issue Date2006
PublisherWorld Scientific Publishing Co Pte Ltd. The Journal's web site is located at http://www.worldscinet.com/ajcm/ajcm.shtml
Citation
American Journal Of Chinese Medicine, 2006, v. 34 n. 5, p. 833-844 How to Cite?
AbstractChinese herbal medicine has been used for thousands of years in China and other Asian countries to treat a variety of inflammatory diseases. The classic Chinese herbal formula, Huo Luo Xiao Ling Dan (HLXL) is commonly used in traditional Chinese herbal medicine for the treatment of joint pain and other symptoms of arthritis. The present study is an investigation of the effects of a modified HLXL extract on persistent hyperalgesia and edema in rats with peripheral inflammation. Inflammation was induced by injecting complete Freund's adjuvant (CFA) into one hind paw. Four dosages of the extract were compared to a vehicle control. Each was administered intragastrally (i.g.) daily for seven days beginning one day before CFA. Hyperalgesia was assessed using a paw withdrawal latency (PWL) test and edema was determined by measuring paw thickness at pre-CFA and 2 hours, 24 hours, and 5 days post-CFA. Immunohistochemistry was performed 2 hours post-CFA to determine spinal Fos protein expression. Adverse effects of the extract were monitored by observing the animals closely for unusual behavioral changes. Compared to the control, HLXL at the two lower dosages (0.575 g/kg and 1.15 g/kg) were effective in the later stage (day 5) of inflammatory hyperalgesia and edema, while the two higher dosages (2.3 g/kg and 4.6 g/kg) alleviated early stage hind paw inflammation and hyperalgesia and facilitated recovery from paw edema and hyperalgesia during the late stage. HLXL at 2.30 g/kg significantly suppressed Fos expression in laminae I-II, III-IV and V-VI ipsilaterally and in III-IV contralaterally. No significant signs of toxicity or adverse effects were observed. The data suggest that HLXL dosage-dependently attenuates CFA-induced inflammation and hyperalgesia, at least in part by inhibiting noxious transmission at the dorsal horn of the spinal cord. © 2006 World Scientific Publishing Company Institute for Advanced Research in Asian Science and Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/188585
ISSN
2015 Impact Factor: 2.959
2015 SCImago Journal Rankings: 0.797
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLao, Len_US
dc.contributor.authorFan, AYen_US
dc.contributor.authorZhang, RXen_US
dc.contributor.authorZhou, Aen_US
dc.contributor.authorMa, ZZen_US
dc.contributor.authorLee, DYWen_US
dc.contributor.authorRen, Ken_US
dc.contributor.authorBerman, Ben_US
dc.date.accessioned2013-09-03T04:10:27Z-
dc.date.available2013-09-03T04:10:27Z-
dc.date.issued2006en_US
dc.identifier.citationAmerican Journal Of Chinese Medicine, 2006, v. 34 n. 5, p. 833-844en_US
dc.identifier.issn0192-415Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/188585-
dc.description.abstractChinese herbal medicine has been used for thousands of years in China and other Asian countries to treat a variety of inflammatory diseases. The classic Chinese herbal formula, Huo Luo Xiao Ling Dan (HLXL) is commonly used in traditional Chinese herbal medicine for the treatment of joint pain and other symptoms of arthritis. The present study is an investigation of the effects of a modified HLXL extract on persistent hyperalgesia and edema in rats with peripheral inflammation. Inflammation was induced by injecting complete Freund's adjuvant (CFA) into one hind paw. Four dosages of the extract were compared to a vehicle control. Each was administered intragastrally (i.g.) daily for seven days beginning one day before CFA. Hyperalgesia was assessed using a paw withdrawal latency (PWL) test and edema was determined by measuring paw thickness at pre-CFA and 2 hours, 24 hours, and 5 days post-CFA. Immunohistochemistry was performed 2 hours post-CFA to determine spinal Fos protein expression. Adverse effects of the extract were monitored by observing the animals closely for unusual behavioral changes. Compared to the control, HLXL at the two lower dosages (0.575 g/kg and 1.15 g/kg) were effective in the later stage (day 5) of inflammatory hyperalgesia and edema, while the two higher dosages (2.3 g/kg and 4.6 g/kg) alleviated early stage hind paw inflammation and hyperalgesia and facilitated recovery from paw edema and hyperalgesia during the late stage. HLXL at 2.30 g/kg significantly suppressed Fos expression in laminae I-II, III-IV and V-VI ipsilaterally and in III-IV contralaterally. No significant signs of toxicity or adverse effects were observed. The data suggest that HLXL dosage-dependently attenuates CFA-induced inflammation and hyperalgesia, at least in part by inhibiting noxious transmission at the dorsal horn of the spinal cord. © 2006 World Scientific Publishing Company Institute for Advanced Research in Asian Science and Medicine.en_US
dc.languageengen_US
dc.publisherWorld Scientific Publishing Co Pte Ltd. The Journal's web site is located at http://www.worldscinet.com/ajcm/ajcm.shtmlen_US
dc.relation.ispartofAmerican Journal of Chinese Medicineen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnti-Inflammatory Agents - Pharmacologyen_US
dc.subject.meshArthritis, Experimental - Chemically Induced - Metabolism - Prevention & Controlen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshDrugs, Chinese Herbal - Pharmacologyen_US
dc.subject.meshEdema - Chemically Induced - Prevention & Controlen_US
dc.subject.meshFreund's Adjuvanten_US
dc.subject.meshHindlimb - Drug Effects - Pathologyen_US
dc.subject.meshHot Temperatureen_US
dc.subject.meshHyperalgesia - Metabolism - Prevention & Controlen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshNeurons - Chemistry - Drug Effects - Pathologyen_US
dc.subject.meshProto-Oncogene Proteins C-Fos - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSpinal Cord - Chemistry - Pathologyen_US
dc.titleAnti-hyperalgesic and anti-inflammatory effects of the modified Chinese herbal formula Huo Luo Xiao Ling Dan (HLXL) in ratsen_US
dc.typeArticleen_US
dc.identifier.emailLao, L: lxlao1@hku.hken_US
dc.identifier.authorityLao, L=rp01784en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1142/S0192415X06004326en_US
dc.identifier.pmid17080548-
dc.identifier.scopuseid_2-s2.0-33750437307en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33750437307&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume34en_US
dc.identifier.issue5en_US
dc.identifier.spage833en_US
dc.identifier.epage844en_US
dc.identifier.isiWOS:000241972000010-
dc.publisher.placeSingaporeen_US
dc.identifier.scopusauthoridLao, L=7005681883en_US
dc.identifier.scopusauthoridFan, AY=7005672886en_US
dc.identifier.scopusauthoridZhang, RX=7404864527en_US
dc.identifier.scopusauthoridZhou, A=55161019400en_US
dc.identifier.scopusauthoridMa, ZZ=8709903000en_US
dc.identifier.scopusauthoridLee, DYW=55808069906en_US
dc.identifier.scopusauthoridRen, K=7102272533en_US
dc.identifier.scopusauthoridBerman, B=35458606800en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats