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Article: Evidence for two aspartate transport systems in prostate epithelial cells

TitleEvidence for two aspartate transport systems in prostate epithelial cells
Authors
Issue Date1990
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304
Citation
Prostate, 1990, v. 16 n. 2, p. 137-145 How to Cite?
AbstractThe transport of aspartate was measured in isolated prostate cells. Two kinetically distinct uptake systems of high (K(m) = 10.0 μM) and low (K(m) = 0.8 mM) affinity were observed. Transport by both systems was Na± dependent and saturable. The tissue distribution of aspartate was also determined by measuring the washout kinetics of endogenous and radio-labeled aspartate from prostate fragments. The distribution of aspartate resulted in 47% of aspartate in the intracellular compartment and 52% in the extracellular (presumably) lumenal compartment. The distribution of aspartate resulted in a calculated intracellular to plasma concentration gradient of approximately 43. We concluded that prostate cells maintain a considerable intracellular to plasma gradient for aspartate and that these cells contain a transport mechanism capable of uptake of aspartate against this considerable gradient.
Persistent Identifierhttp://hdl.handle.net/10722/188523
ISSN
2015 Impact Factor: 3.778
2015 SCImago Journal Rankings: 1.477
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFranklin, RBen_US
dc.contributor.authorLao, Len_US
dc.contributor.authorCostello, LCen_US
dc.date.accessioned2013-09-03T04:10:04Z-
dc.date.available2013-09-03T04:10:04Z-
dc.date.issued1990en_US
dc.identifier.citationProstate, 1990, v. 16 n. 2, p. 137-145en_US
dc.identifier.issn0270-4137en_US
dc.identifier.urihttp://hdl.handle.net/10722/188523-
dc.description.abstractThe transport of aspartate was measured in isolated prostate cells. Two kinetically distinct uptake systems of high (K(m) = 10.0 μM) and low (K(m) = 0.8 mM) affinity were observed. Transport by both systems was Na± dependent and saturable. The tissue distribution of aspartate was also determined by measuring the washout kinetics of endogenous and radio-labeled aspartate from prostate fragments. The distribution of aspartate resulted in 47% of aspartate in the intracellular compartment and 52% in the extracellular (presumably) lumenal compartment. The distribution of aspartate resulted in a calculated intracellular to plasma concentration gradient of approximately 43. We concluded that prostate cells maintain a considerable intracellular to plasma gradient for aspartate and that these cells contain a transport mechanism capable of uptake of aspartate against this considerable gradient.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304en_US
dc.relation.ispartofProstateen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAspartic Acid - Blood - Metabolismen_US
dc.subject.meshBiological Transporten_US
dc.subject.meshCarrier Proteins - Metabolismen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEpithelial Cellsen_US
dc.subject.meshEpithelium - Metabolismen_US
dc.subject.meshIntracellular Membranes - Metabolismen_US
dc.subject.meshKineticsen_US
dc.subject.meshMaleen_US
dc.subject.meshOsmolar Concentrationen_US
dc.subject.meshProstate - Cytology - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshSodium - Pharmacologyen_US
dc.subject.meshTissue Distributionen_US
dc.titleEvidence for two aspartate transport systems in prostate epithelial cellsen_US
dc.typeArticleen_US
dc.identifier.emailLao, L: lxlao1@hku.hken_US
dc.identifier.authorityLao, L=rp01784en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/pros.2990160205en_US
dc.identifier.pmid2320504-
dc.identifier.scopuseid_2-s2.0-0025170710en_US
dc.identifier.volume16en_US
dc.identifier.issue2en_US
dc.identifier.spage137en_US
dc.identifier.epage145en_US
dc.identifier.isiWOS:A1990CW63100004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridFranklin, RB=7201428474en_US
dc.identifier.scopusauthoridLao, L=7005681883en_US
dc.identifier.scopusauthoridCostello, LC=24592383900en_US

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