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Conference Paper: Soluble Receptor for Advanced Glycation End-Products in Chinese Type 1 Diabetic Patients

TitleSoluble Receptor for Advanced Glycation End-Products in Chinese Type 1 Diabetic Patients
Authors
Issue Date2012
PublisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=2040-1116&site=1
Citation
The 9th International Diabetes Federation Western Pacific Region Congress (IDF-WPR), and 4th Scientific Meeting of the Asian Association for the Study of Diabetes (AASD), Kyoto, Japan, 24-27 November 2012. In Journal of Diabetes Investigation, 2012, v. 3 n. Suppl.1, p. 212, abstract no. PCS-24-5 How to Cite?
AbstractOBJECTIVES: The receptor for advanced glycation end-products (RAGE) plays an important role in the pathogenesis of diabetic complications. Interfering with the activation of RAGE by using a soluble form of the receptor (sRAGE) ameliorates the vascular complications of diabetes in animal models. sRAGE in the human circulation is partly derived from ectodomain shedding of the membrane-associated receptor. We have investigated the effect of insulin on the generation of sRAGE in vitro and evaluated serum levels of sRAGE in a group of Chinese type 1 diabetic subjects. METHODS: THP-1 cells were incubated with insulin in vitro and sRAGE in the medium measured by Western immunoblot. Cell-surface biotinylation and immunoprecipitation was performed to investigate ectodomain shedding. Serum level of sRAGE in diabetic patients was measured by ELISA. RESULTS: Insulin increased sRAGE in cell conditioned-media in a time- and dose-dependent manner. Pretreatment of THP-1 cells with GM6001, a general metalloproteinase inhibitor which blocked cellular shedding events, significantly reduced the production of sRAGE. This would suggest that the increase in sRAGE upon incubation with insulin was partly due to the shedding of sRAGE by cleavage of full-length cell surface RAGE. In type 1 diabetic patients (n = 120), serum sRAGE was higher compared to a group of age-matched non-diabetic healthy subjects (1021.6 pg/mL [724.8–1212.4.0] vs 812.9 pg/mL [548.0–1202.2.5] respectively, P = 0.004). CONCLUSIONS: Chinese type 1 diabetic patients have higher serum levels of sRAGE and we have shown that insulin can stimulate the ectodomain shedding of cell surface RAGE.
DescriptionPoster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/186857
ISSN
2015 Impact Factor: 2.294
2015 SCImago Journal Rankings: 0.964

 

DC FieldValueLanguage
dc.contributor.authorLam, KYJen_US
dc.contributor.authorWang, Yen_US
dc.contributor.authorShiu, SWMen_US
dc.contributor.authorWong, Yen_US
dc.contributor.authorTan, KCBen_US
dc.date.accessioned2013-08-20T12:21:15Z-
dc.date.available2013-08-20T12:21:15Z-
dc.date.issued2012en_US
dc.identifier.citationThe 9th International Diabetes Federation Western Pacific Region Congress (IDF-WPR), and 4th Scientific Meeting of the Asian Association for the Study of Diabetes (AASD), Kyoto, Japan, 24-27 November 2012. In Journal of Diabetes Investigation, 2012, v. 3 n. Suppl.1, p. 212, abstract no. PCS-24-5en_US
dc.identifier.issn2040-1116-
dc.identifier.urihttp://hdl.handle.net/10722/186857-
dc.descriptionPoster Presentation-
dc.description.abstractOBJECTIVES: The receptor for advanced glycation end-products (RAGE) plays an important role in the pathogenesis of diabetic complications. Interfering with the activation of RAGE by using a soluble form of the receptor (sRAGE) ameliorates the vascular complications of diabetes in animal models. sRAGE in the human circulation is partly derived from ectodomain shedding of the membrane-associated receptor. We have investigated the effect of insulin on the generation of sRAGE in vitro and evaluated serum levels of sRAGE in a group of Chinese type 1 diabetic subjects. METHODS: THP-1 cells were incubated with insulin in vitro and sRAGE in the medium measured by Western immunoblot. Cell-surface biotinylation and immunoprecipitation was performed to investigate ectodomain shedding. Serum level of sRAGE in diabetic patients was measured by ELISA. RESULTS: Insulin increased sRAGE in cell conditioned-media in a time- and dose-dependent manner. Pretreatment of THP-1 cells with GM6001, a general metalloproteinase inhibitor which blocked cellular shedding events, significantly reduced the production of sRAGE. This would suggest that the increase in sRAGE upon incubation with insulin was partly due to the shedding of sRAGE by cleavage of full-length cell surface RAGE. In type 1 diabetic patients (n = 120), serum sRAGE was higher compared to a group of age-matched non-diabetic healthy subjects (1021.6 pg/mL [724.8–1212.4.0] vs 812.9 pg/mL [548.0–1202.2.5] respectively, P = 0.004). CONCLUSIONS: Chinese type 1 diabetic patients have higher serum levels of sRAGE and we have shown that insulin can stimulate the ectodomain shedding of cell surface RAGE.-
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=2040-1116&site=1-
dc.relation.ispartofJournal of Diabetes Investigationen_US
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.titleSoluble Receptor for Advanced Glycation End-Products in Chinese Type 1 Diabetic Patientsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLam, KYJ: lamkyj@hku.hken_US
dc.identifier.emailWang, Y: yuwanghk@hku.hken_US
dc.identifier.emailShiu, SWM: swmshiu@hku.hken_US
dc.identifier.emailWong, Y: ywong@hku.hken_US
dc.identifier.emailTan, KCB: kcbtan@hku.hken_US
dc.identifier.authorityWang, Y=rp00239en_US
dc.identifier.authorityTan, KCB=rp00402en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/jdi.12013-
dc.identifier.hkuros220908en_US
dc.identifier.volume3-
dc.identifier.issueSuppl.1-
dc.identifier.spage212, abstract no. PCS-24-5-
dc.identifier.epage212, abstract no. PCS-24-5-
dc.publisher.placeAustralia-

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