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Conference Paper: Decorin regulates cell activation and fibrotic processes in human peritoneal mesothelial cells through inhibition of ERK phosphorylation

TitleDecorin regulates cell activation and fibrotic processes in human peritoneal mesothelial cells through inhibition of ERK phosphorylation
Authors
Issue Date2012
PublisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org
Citation
The 2012 Annual Meeting and Scientific Exposition of the American Society of Nephrology (Kidney Week 2012), San Diego, CA., 30 October-4 November 2012. In Journal of the American Society of Nephrology, 2012, v. 23 abstract suppl., p. 550A, abstract FR-PO793 How to Cite?
AbstractBACKGROUND: Mesothelial cell dysfunction contributes to peritoneal fibrosis during long-term peritoneal dialysis (PD). We previously demonstrated that mesothelial cells synthesize abundant decorin, a dermatan sulfate proteoglycan with anti-fibrotic properties. In this study, we investigated the mechanisms through which decorin regulates fibrogenesis and epithelial-to-mesenchymal transition (EMT) in human peritoneal mesothelial cells (HPMC) in the setting of PD. METHODS: Growth arrested HPMC were stimulated with spent non-infected or infected PD fluid in the presence or absence of exogenous decorin (0-1000 ng/ml) for 24h. Cell morphology, expression of fibronectin and fibroblast specific protein-1, and ERK activation were assessed. The effect of decorin gene silencing on cell activation and fibrotic processes in HPMC was also investigated. RESULTS: Non-infected PDF induced EMT and cell detachment in HPMC. These changes were more marked in cells exposed to infected PD fluid. Pre-incubation of HPMC with decorin preserved HPMC morphology and inhibited PD fluid-induced EMT, accompanied by decreased fibronectin synthesis and increased E-cadherin expression. The effect of decorin was mediated through the suppression of ERK phosphorylation. Knockdown of decorin expression in HPMC resulted in increased expression of fibroblast specific protein-1, fibronectin and TGF-β1 in both control and PD fluid stimulated HPMC (P<0.01, for all), accompanied by decreased E-cadherin expression (P<0.01, for all). CONCLUSIONS: The data demonstrate that decorin regulates cell activation and fibrotic processes in HPMC during PD through modulation of the ERK signaling pathway.
DescriptionMeeting Theme: Curing Kidney Disease
Session - Peritoneal Dialysis - I: From Membrane to Clinical Outcome: abstract FR-PO793
Persistent Identifierhttp://hdl.handle.net/10722/186843
ISSN
2015 Impact Factor: 8.491
2015 SCImago Journal Rankings: 4.699

 

DC FieldValueLanguage
dc.contributor.authorYung, Sen_US
dc.contributor.authorChau, Men_US
dc.contributor.authorZhang, Qen_US
dc.contributor.authorYim, Aen_US
dc.contributor.authorJiang, Nen_US
dc.contributor.authorChan, DTMen_US
dc.date.accessioned2013-08-20T12:21:13Z-
dc.date.available2013-08-20T12:21:13Z-
dc.date.issued2012en_US
dc.identifier.citationThe 2012 Annual Meeting and Scientific Exposition of the American Society of Nephrology (Kidney Week 2012), San Diego, CA., 30 October-4 November 2012. In Journal of the American Society of Nephrology, 2012, v. 23 abstract suppl., p. 550A, abstract FR-PO793en_US
dc.identifier.issn1046-6673-
dc.identifier.urihttp://hdl.handle.net/10722/186843-
dc.descriptionMeeting Theme: Curing Kidney Disease-
dc.descriptionSession - Peritoneal Dialysis - I: From Membrane to Clinical Outcome: abstract FR-PO793-
dc.description.abstractBACKGROUND: Mesothelial cell dysfunction contributes to peritoneal fibrosis during long-term peritoneal dialysis (PD). We previously demonstrated that mesothelial cells synthesize abundant decorin, a dermatan sulfate proteoglycan with anti-fibrotic properties. In this study, we investigated the mechanisms through which decorin regulates fibrogenesis and epithelial-to-mesenchymal transition (EMT) in human peritoneal mesothelial cells (HPMC) in the setting of PD. METHODS: Growth arrested HPMC were stimulated with spent non-infected or infected PD fluid in the presence or absence of exogenous decorin (0-1000 ng/ml) for 24h. Cell morphology, expression of fibronectin and fibroblast specific protein-1, and ERK activation were assessed. The effect of decorin gene silencing on cell activation and fibrotic processes in HPMC was also investigated. RESULTS: Non-infected PDF induced EMT and cell detachment in HPMC. These changes were more marked in cells exposed to infected PD fluid. Pre-incubation of HPMC with decorin preserved HPMC morphology and inhibited PD fluid-induced EMT, accompanied by decreased fibronectin synthesis and increased E-cadherin expression. The effect of decorin was mediated through the suppression of ERK phosphorylation. Knockdown of decorin expression in HPMC resulted in increased expression of fibroblast specific protein-1, fibronectin and TGF-β1 in both control and PD fluid stimulated HPMC (P<0.01, for all), accompanied by decreased E-cadherin expression (P<0.01, for all). CONCLUSIONS: The data demonstrate that decorin regulates cell activation and fibrotic processes in HPMC during PD through modulation of the ERK signaling pathway.-
dc.languageengen_US
dc.publisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org-
dc.relation.ispartofJournal of the American Society of Nephrologyen_US
dc.titleDecorin regulates cell activation and fibrotic processes in human peritoneal mesothelial cells through inhibition of ERK phosphorylationen_US
dc.typeConference_Paperen_US
dc.identifier.emailYung, S: ssyyung@hku.hken_US
dc.identifier.emailChau, M: melchau@hkucc.hku.hken_US
dc.identifier.emailZhang, Q: zhjhr@hkucc.hku.hken_US
dc.identifier.emailYim, A: anndyim@hku.hken_US
dc.identifier.emailChan, DTM: dtmchan@hku.hken_US
dc.identifier.authorityYung, S=rp00455en_US
dc.identifier.authorityChan, DTM=rp00394en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros220420en_US
dc.identifier.volume23en_US
dc.identifier.issueabstract suppl.-
dc.identifier.spage550Aen_US
dc.identifier.epage550Aen_US
dc.publisher.placeUnited States-

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