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Conference Paper: Viability of intra-tubular bacteria following chemomechanical caries removal methods

TitleViability of intra-tubular bacteria following chemomechanical caries removal methods
Authors
KeywordsBacterial
Caries
Chemomechanical and Enzymes
Issue Date2013
PublisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925
Citation
The 91st General Session General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92, Special Issue A, abstract no. 3278 How to Cite?
AbstractObjectives: To assess the bacterial viability inside dentinal tubules following the application of sodium hypochlorite-based (Carisolv gel) and enzymatic-based (Papacarie gel) chemomechanical caries removal agents. Methods: Twenty-five caries-free dentine discs (2 mm thickness) prepared from 25 sound maxillary premolars were used. The discs were ultrasonically cleaned in 10% citric acid and 5% sodium hypochlorite (NaOCl) solution respectively for 4 minutes each to remove the smear layer. The discs were then infected with Streptococcus mutans suspension [3 x 106colony-forming unit (CFU)/mL] using the centrifugation method. The dentine discs were randomly divided into 5 groups according to the dentine treatments. In Group A, the discs were left untreated to act as negative control. Group B was treated with 5% NaOCl solution; Group C: 2% Chlorhexidine (CHX) solution; Group D: Carisolv gel and Group E: Papacarie gel. All agents were applied for 5 minutes. The dentine discs were fractured into two sections and stained with fluorescent LIVE/DEAD Baclight Bacterial Viability Stain (Molecular Probes, Eugene, OR). Each specimen was observed using confocal laser scanning microscopy (CLSM) (Fluoview 1000, Olympus, PA, USA) at five different randomly selected sites. Representative fractured specimens were observed under SEM for evaluation of intra-tubular bacterial penetration. Results: One-way ANOVA and Tukey post hoc multiple comparison tests revealed that 5% NaOCl solution achieved the highest intra-tubular antibacterial effect; while Carisolv gel had the lowest antibacterial effect (P<0.05). No significant difference in antibacterial effect was observed between Papacarie gel and 2% CHX solution (P>0.05). Conclusions: Papacarie enzymatic-based chemomechanical caries removal gel has an antibacterial effect similar to 2% chlorhexidine solution. Papacarie gel is effective in the reduction of residual cariogenic bacteria in the dentinal tubules.
DescriptionPoster Session: Cariogenic Biofilms Composition and Properties
Persistent Identifierhttp://hdl.handle.net/10722/186508
ISSN
2015 Impact Factor: 4.602
2015 SCImago Journal Rankings: 1.714

 

DC FieldValueLanguage
dc.contributor.authorHamama, HHHEen_US
dc.contributor.authorYiu, CKYen_US
dc.contributor.authorBurrow, MFen_US
dc.date.accessioned2013-08-20T12:12:09Z-
dc.date.available2013-08-20T12:12:09Z-
dc.date.issued2013en_US
dc.identifier.citationThe 91st General Session General Session & Exhibition of the International Association for Dental Research (IADR), Seattle, Washington, USA, 20-23 March 2013. In Journal of Dental Research, 2013, v. 92, Special Issue A, abstract no. 3278en_US
dc.identifier.issn0022-0345-
dc.identifier.urihttp://hdl.handle.net/10722/186508-
dc.descriptionPoster Session: Cariogenic Biofilms Composition and Properties-
dc.description.abstractObjectives: To assess the bacterial viability inside dentinal tubules following the application of sodium hypochlorite-based (Carisolv gel) and enzymatic-based (Papacarie gel) chemomechanical caries removal agents. Methods: Twenty-five caries-free dentine discs (2 mm thickness) prepared from 25 sound maxillary premolars were used. The discs were ultrasonically cleaned in 10% citric acid and 5% sodium hypochlorite (NaOCl) solution respectively for 4 minutes each to remove the smear layer. The discs were then infected with Streptococcus mutans suspension [3 x 106colony-forming unit (CFU)/mL] using the centrifugation method. The dentine discs were randomly divided into 5 groups according to the dentine treatments. In Group A, the discs were left untreated to act as negative control. Group B was treated with 5% NaOCl solution; Group C: 2% Chlorhexidine (CHX) solution; Group D: Carisolv gel and Group E: Papacarie gel. All agents were applied for 5 minutes. The dentine discs were fractured into two sections and stained with fluorescent LIVE/DEAD Baclight Bacterial Viability Stain (Molecular Probes, Eugene, OR). Each specimen was observed using confocal laser scanning microscopy (CLSM) (Fluoview 1000, Olympus, PA, USA) at five different randomly selected sites. Representative fractured specimens were observed under SEM for evaluation of intra-tubular bacterial penetration. Results: One-way ANOVA and Tukey post hoc multiple comparison tests revealed that 5% NaOCl solution achieved the highest intra-tubular antibacterial effect; while Carisolv gel had the lowest antibacterial effect (P<0.05). No significant difference in antibacterial effect was observed between Papacarie gel and 2% CHX solution (P>0.05). Conclusions: Papacarie enzymatic-based chemomechanical caries removal gel has an antibacterial effect similar to 2% chlorhexidine solution. Papacarie gel is effective in the reduction of residual cariogenic bacteria in the dentinal tubules.-
dc.languageengen_US
dc.publisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925-
dc.relation.ispartofJournal of Dental Researchen_US
dc.rightsJournal of Dental Research. Copyright © Sage Publications, Inc..-
dc.subjectBacterial-
dc.subjectCaries-
dc.subjectChemomechanical and Enzymes-
dc.titleViability of intra-tubular bacteria following chemomechanical caries removal methodsen_US
dc.typeConference_Paperen_US
dc.identifier.emailYiu, CKY: ckyyiu@hkucc.hku.hken_US
dc.identifier.emailBurrow, MF: mfburr58@hku.hken_US
dc.identifier.authorityYiu, CKY=rp00018en_US
dc.identifier.authorityBurrow, MF=rp01306en_US
dc.identifier.hkuros216886en_US
dc.identifier.volume92-
dc.identifier.issueSpecial Issue A-
dc.publisher.placeUnited States-

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